Esansiyel Yağların Eklenmesinin, Sığırlardan İzole Edilen Rumen Funguslarının Yaşama Oranları ve Lignoselülolitik Enzim Aktivitelerine Etkileri

Abstract

Due to the ban on antibiotics and ionophores, which were used as feed supplements, scientists have become more interested in evaluating other alternatives to control the rumen fermentation. Plant essential oils might be alternative to that kind of supplements for farm animals. However, our knowledge about the possible effects of plant essential oils on survival rate and enzyme activity of anaerobic fungi (besides other rumen microbes) is still limited. Current study, therefore, aimed to determine the effects of plant essential oils on this unique group of rumen microorganisms. Effects of various dosages of myrtle (Myrtus communis), juniper (Juniperus communis), melissa (Melissa officinalis) and thyme (Thymus vulgaris) plant essential oil, which have antimicrobial properties, were investigated on survival rate and enzyme activity of Neocallimastix sp. GMLF2 and Orpinomyces sp. GMLF18. Accordingly, plant essential oils were dissolved in ethyl alcohol and added into medium. Fungal growth (Neocallimastix sp. GMLF2 and Orpinomyces sp. GMLF18) was completely inhibited in the 1 mg/ml essential oil concentration of culture medium. Furthermore carboxymethylcellulase and xylanase activities were decreased remarkably when 0.5 mg/ml concentration of essential oil was added into culture media regardless of type of the oil.