ESBL- and Carbapenemase-Producing <i>Enterobacteriaceae</i> in Patients with Bacteremia, Yangon, Myanmar, 2014

Myat, Tin Ohn; Hannaway, Rachel F.; Zin, Khwar Nyo; Htike, Wah Win; Win, Kyu Kyu; Crump, John A.; Murdoch, David R.; Ussher, James E.

doi:10.3201/eid2305.161100

Cited by 27 publications

(24 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phenotypic testing of ESBL and carbapenemase production by the isolated E. coli showed that out of the 88 isolates; 49 patients (55.68%) were ESBL producers and 30 (34.09%) were carbapenemase producers. This finding is consistent with the high proportion of ESBL production reported in a study conducted in Egypt also, showing more than half (54.5%) of isolated E. coli positive for ESBL [20] and in New Zealand as about half of E. coli isolates produced ESBL [21]. Similar results in the USA [22] and India [23] have been reported as prevalence rates of ESBL were from 35.0 to 42% for E. coli.…”

Section: Discussionsupporting

confidence: 89%

Genetic Study of Extended Spectrum Beta-Lactamase and Carbapenemase Producing Escherichia Coli Causing Sepsis among Egyptian Children

Zaki¹,

El-Halaby²,

Elmansoury³

et al. 2019

TOMICROJ

View full text Add to dashboard Cite

Background: Treatment failure of sepsis caused by Escherichia coli (E. Coli) is a leading cause of death of infants and children in intensive care units. Objective: To detect the prevalence of Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase-genes between E. coli isolates from infants and children with septicemia and to identify their antibiotic sensitivity pattern. Methods: This is a cross-sectional study performed on 88 patients with sepsis. The isolated E. coli were identified by Gram stain and biochemically by the Microscan automated system. ESBL and carbapenemase producing E. coli were isolated on double disk diffusion and EDTA double disk, respectively. Polymerase chain reaction for ESBL and carbapenemase producing E. coli genes were performed. Bacterial susceptibility to antibiotics was tested. The initial results were measured through the 30-days of hospital admission. IRB approved the study. Results: Of 88 patients with sepsis, 49 and 30 strains were ESBL producing and carbapenemase producing E. coli; respectively. Neither risk factors for infection nor clinical picture can differentiate between ESBL and carbapenemase producing E. coli. The most frequently detected gene of ESBL producing E. coli was SHV, it was more sensitive to Piperacillin/Tazobactam (90%) and cefepime (86.7%) while for carbapenemase-producing E. coli; IMP was the most frequent, its sensitivity was high to Piperacillin/Tazobactam and Ciprofloxacin (52.6% each). Conclusion: The commonest gene of ESBL producing E. coli is SHV whereas for carbapenemase-producing E. coli is IMP. Piperacillin/Tazobactam is the candidate drug to start in children with septicemia and suspected ESBL or carbapenemase-producing E. coli infection.

show abstract

Section: Discussionsupporting

confidence: 89%

Genetic Study of Extended Spectrum Beta-Lactamase and Carbapenemase Producing Escherichia Coli Causing Sepsis among Egyptian Children

Zaki¹,

El-Halaby²,

Elmansoury³

et al. 2019

TOMICROJ

View full text Add to dashboard Cite

show abstract

“…In a survey conducted in a tertiary-care hospital and two private hospitals in Yangon, Myanmar, Escherichia coli and K. pneumoniae isolates carrying bla NDM genes were isolated (17). E. coli isolates carrying the bla NDM genes were also found in another hospital in Yangon (18), suggesting that organisms carrying these genes are spreading in the region.…”

mentioning

confidence: 99%

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Sugawara

Akeda

Hagiya

et al. 2019

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The spread of carbapenemase-producing Enterobacteriaceae (CPE), contributing to widespread carbapenem resistance, has become a global concern. However, the specific dissemination patterns of carbapenemase genes have not been intensively investigated in developing countries, including Myanmar, where NDM-type carbapenemases are spreading in clinical settings. In the present study, we phenotypically and genetically characterized 91 CPE isolates obtained from clinical (n = 77) and environmental (n = 14) samples in Yangon, Myanmar. We determined the dissemination of plasmids harboring genes encoding NDM-1 and its variants using whole-genome sequencing and plasmid analysis. IncFII plasmids harboring blaNDM-5 and IncX3 plasmids harboring blaNDM-4 or blaNDM-7 were the most prevalent plasmid types identified among the isolates. The IncFII plasmids were predominantly carried by clinical isolates of Escherichia coli, and their clonal expansion was observed within the same ward of a hospital. In contrast, the IncX3 plasmids were found in phylogenetically divergent isolates from clinical and environmental samples classified into nine species, suggesting widespread dissemination of plasmids via horizontal transfer. Half of the environmental isolates were found to possess IncX3 plasmids, and this type of plasmid was confirmed to transfer more effectively to recipient organisms at a relatively low temperature (25°C) compared to the IncFII plasmid. Moreover, various other plasmid types were identified harboring blaNDM-1, including IncFIB, IncFII, IncL/M, and IncA/C2, among clinical isolates of Klebsiella pneumoniae or Enterobacter cloacae complex. Overall, our results highlight three distinct patterns of the dissemination of blaNDM-harboring plasmids among CPE isolates in Myanmar, contributing to a better understanding of their molecular epidemiology and dissemination in a setting of endemicity.

show abstract

“…1. Antibiotic resistance is a burning issue in the field of medicine which complicate treatment of infected patients (19) . One of the mechanisms by which Gram-negative bacteria acquire resistance to beta-lactam antibiotics is through the production of betalactamases.…”

Section: Resultsmentioning

confidence: 99%

Beta‐lactamase‐producing Escherichia coli in Bangladesh: Their phenotypic and molecular characteristics

Ahsan

Islam

2019

Dhaka Univ. J. Biol. Sci

View full text Add to dashboard Cite

The emergence and rapid dissemination of beta-lactamase-producing E. coli is now a worldwide problem. A total of 45 E. coli obtained from clinical specimens from a medical service centre in Dhaka were selected for this study. Test E. coli exhibited variable resistance to 3rd (71.7 - 97.8%, n = 48) and 4th (78%, n = 48) generation beta-lactam antibiotics, with 72% sensitivity to Carbapenem. Analysis of co-resistance indicated that 33.3% of E. coli (n = 48) were co-resistant to beta-lactams and ciprofloxacin. ESBL producers were predominant comprising of 84.7% E. coli. Among them, 22.7% contained blaTEM, 24.2% contained blaCTX-M, 4.3% contained blaSHV and 9.1% contained blaOXA-1 genes. A total of 25.75% isolates were metallo beta-lactamase producers. Of these, 1.5% of E. coli strains contained New Delhi metallo beta-lactamase gene and 6% contained AmpC gene. Multiple beta-lactamase genes were detected in some test isolates; 6.7% isolates contained 4, 20% contained 3 and 73.3% contained 2 beta lactamase genes. Fifty per cent of the E. coli contained plasmids of variable sizes. In addition, a total of 39% of the E. coli contained Class 1 integron. The increasing trend in beta-lactam resistance is of public health concern as it limits treatment regime and indicates to the need of continuous monitoring of resistance pattern. Dhaka Univ. J. Biol. Sci. 28(1): 71-81, 2019 (January)

show abstract

ESBL- and Carbapenemase-Producing Enterobacteriaceae in Patients with Bacteremia, Yangon, Myanmar, 2014

Cited by 27 publications

References 8 publications

Genetic Study of Extended Spectrum Beta-Lactamase and Carbapenemase Producing Escherichia Coli Causing Sepsis among Egyptian Children

Genetic Study of Extended Spectrum Beta-Lactamase and Carbapenemase Producing Escherichia Coli Causing Sepsis among Egyptian Children

Spreading Patterns of NDM-Producing Enterobacteriaceae in Clinical and Environmental Settings in Yangon, Myanmar

Beta‐lactamase‐producing Escherichia coli in Bangladesh: Their phenotypic and molecular characteristics

Contact Info

Product

Resources

About