ESBL producing Pseudomonas aeruginosa in clinical specimens: Is it a scary nightmare or paper tiger?

Nithyalakshmi, J; Vidhyarani, R; Mohanakrishnan, K.; Sumathi, G

doi:10.5958/2394-5478.2016.00062.5

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…This result was in agreement with those observed by Hakemi and associates [16] with the percentage of resistance 66% and 67% for both of ceftazidime and cefepime respectively. Nithyalakshmi et al [19] agreed with our study in finding low resistance to imipenem (3.4%). The resistance tool of problematic infections affiliated from P. aeruginosa to imipenem and meropenem may be due to diminished expression of an OprD-like protein which works as a carbapenem-specific channel and expressed by the OprD gene.…”

Section: Discussionsupporting

confidence: 92%

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Al-Ouqaili¹

2018

IJGP

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Background and Objective:The increase in extended-spectrum β-lactamases (ESBLs) producing microbes in recent years has led to the great challenge for the clinician in the treatment. The study aims to investigate the molecular basis of ESBL encoding-resistant gene, SHV to modern β-lactams. Also, to detect gene sequencing and to compare their genetic relatedness utilizing phylogenetic analysis. Patients and Methods: A total of 100 clinical isolates are submitting after identification to phenotypic confirmatory double disk synergy technique. Polymerase chain reaction was performed for the determination of SHV gene, and sequencing analysis for the amplified gene is also achieved. Results: A total of 27 isolates, 3 (11.1%), 15 (55.55%), and 9 (33.0%) of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa were positive for a phenotypic and confirmatory test for ESBLs, respectively. SHV gene was detected only in 5 (31.25%) isolates of P. aeruginosa, 2 (12.5 %) E. coli, and 9 (56.25%) in K. pneumoniae. Conclusions: SHV gene plays an essential role in the resistance of ESBL producer isolates to new β-lactams. The sequencing of this gene revealed 98-99% compatibility range with the global standard gene in National Center of Biotechnology Information.

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Section: Discussionsupporting

confidence: 92%

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Al-Ouqaili¹

2018

IJGP

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“…These variations in ESBL-positive rates may be related to antibiotic use patterns in different geographic locations as well as differences in infection control procedures for hospital personnel in those regions. Moreover, the spread of ESBLproducing P. aeruginosa varies from country to country [22]. In the present study, Pseudomonas isolates showed resistance to ceftriaxone (72.4%), ceftazidime (65.5%), aztreonam (24.1%), and one was resistant to imipenem (3.4%), similar to the results by other investigators [22,25].…”

Section: Discussionsupporting

confidence: 91%

“…Similar to the results obtained by Hakemi et al [21], 34.2% of our P. aeruginosa isolates were ESBL-positive. Other authors [22,23]reported lower rates of ESBL-positive P. aeruginosa isolates and one [24] reported a higher rate. These variations in ESBL-positive rates may be related to antibiotic use patterns in different geographic locations as well as differences in infection control procedures for hospital personnel in those regions.…”

Section: Discussionmentioning

confidence: 99%

DNA Sequence Analysis of BlaVEB Gene Encoding Multi-drug Resistant and Extended-spectrum β-lactamases Producer Isolates of Enterobacteriaceae and Pseudomonas aeruginosa

Al-Ouqaili¹,

Khalaf²,

Al-Kubaisy³

2020

TOMICROJ

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Objective: Multi-drug resistance Gram-negative bacteria possessing Extended-Spectrum β-Lactamase (ESBL) genes are of concern because of their resistance to third-generation cephalosporins. This study aims to investigate the molecular basis of resistance to modern β-lactams by ESBLs encoded by the blaVEB gene and the gene’s role in resistance. Also, gene sequencing was used to compare genetic similarities with global isolates using phylogenetic and cluster analyses. Methods: Between March and July 2018, a total of 100 Iraqi clinical isolates were examined, in this cross-sectional study, to determine their ESBL status using the double-disc synergy technique. Polymerase Chain Reactions (PCRs) were performed on extracted blaVEB genes and sequencing of the target PCR products was performed. All blaVEB sequences were compared with the available sequence data, using BLAST searches against the GenBank database. Results: A total of 35 isolates, comprising 5 Escherichia coli, 18 Klebsiella pneumoniae, and 12 Pseudomonas aeruginosa isolates were confirmed to possess ESBLs; the blaVEB gene was detected in one isolate of each species. The sequencing of these genes revealed 99% similarity with the global standard genes deposited in GenBank. Conclusion: The blaVEB gene plays an essential role in the resistance of ESBL-producing isolates to new β-lactams. Further, the sequencing and phylogenetic analyses of the genes from the P. aeruginosa, K. pneumonia, and E. coli isolates revealed 99% similarity with the GenBank global standard genes.

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“…The highest incidence of MBL production was observed among MDR P. aeruginosa samples isolated from wound infections (19%) followed by those isolated from ear infections (14.3%). Nithyalakshmi et al29 reported that the frequency of occurrence of ESBL among P. aeruginosa isolates was 21.96%, and most ESBL producers were obtained from urine samples (27.7%), followed by respiratory infection (23.68%), and wound infection (22.95%).…”

Section: Resultsmentioning

confidence: 99%

<p>Antimicrobial resistance pattern and molecular genetic distribution of metallo-β-lactamases producing <em>Pseudomonas aeruginosa</em> isolated from hospitals in Minia, Egypt</p>

Farhan¹,

Ibrahim²,

Mahran³

et al. 2019

IDR

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Background: Pseudomonas aeruginosa ( P. aeruginosa ) represents a great threat to public health worldwide, due to its high ability to acquire resistance to different antibiotic classes. Carbapenems are effective against multidrug resistant (MDR) P. aeruginosa, but their widespread use has resulted in the emergence of carbapenem-resistant strains, which is considered a major global concern. This study aimed to determine the prevalence of carbapenem resistance among P. aeruginosa strains isolated from different sites of infection. Methods: Between October 2016 and February 2018, a total of 530 clinical specimens were collected from patients suffering from different infections, then processed and cultured. Isolates were tested for extended spectrum β-lactamase (ESBL) and metallo-β-lactamase (MBL) production using double-disk synergy test, modified Hodge tests, and disc potentiation test. PCR was used for the detection of selected OXA carbapenemases encoding genes. Results: Of 530 samples, 150 (28.3%) P. aeruginosa isolates were obtained. MDR strains were found in 66.6% (100 of 150) of isolates. Of 100 MDR P. aeruginosa isolates, 54 (54%) were ESBL producers and 21 (21%) carbapenem resistant P. aeruginosa . MBL production was found in 52.3% (eleven) carbapenem-resistant isolates. CTX-M15 was found among 55.5% of ESBL- producing P. aeruginosa . Carbapenemase genes detected were bla IMP (42.8%, nine of 21), bla VIM (52.3%, eleven of 21), bla GIM (52.3%, eleven of 21), bla SPM (38%, 8/21). In addition, isolates that were positive for the tested genes showed high resistance to other antimicrobials, such as colistin sulfate and tigecycline. Conclusion: Our study indicates that P. aeruginosa harboring ESBL and MBL with limited sensitivity to antibiotics are common among the isolated strains, which indicates the great problem facing the treatment of serious infectious diseases. As such, there is a need to study the resistance patterns of isolates and carry out screening for the presence of ESBL and MBL enzymes, in order to choose the proper antibiotic.

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ESBL producing Pseudomonas aeruginosa in clinical specimens: Is it a scary nightmare or paper tiger?

Cited by 5 publications

References 11 publications

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

Molecular Detection and Sequencing of SHV Gene Encoding for Extended-Spectrum Β-Lactamases Produced by Multidrug Resistance Some of the Gram-Negative Bacteria

DNA Sequence Analysis of BlaVEB Gene Encoding Multi-drug Resistant and Extended-spectrum β-lactamases Producer Isolates of Enterobacteriaceae and Pseudomonas aeruginosa

<p>Antimicrobial resistance pattern and molecular genetic distribution of metallo-β-lactamases producing <em>Pseudomonas aeruginosa</em> isolated from hospitals in Minia, Egypt</p>

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