Escherichia coli 0157: outbreak in central Scotland

Liddell, K. G.

doi:10.1016/s0140-6736(05)61214-7

Cited by 10 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Outbreaks of STEC O157 associated with contamination at a butcher’s premises have been described previously [ 11, 12 ]. However, during this investigation, the source of the contaminated food was traced back to the farm supplying the cattle to the implicated butchers for slaughter.…”

Section: Discussionmentioning

confidence: 99%

Farm-to-fork investigation of an outbreak of Shiga toxin-producing Escherichia coli O157

et al. 2018

View full text Add to dashboard Cite

Fifteen cases of Shiga toxin-producing Escherichia coli (STEC) O157 infection were associated with the consumption of contaminated food from two related butchers’ premises in the north-east of England. Ten cases were admitted to hospital and seven cases developed haemolytic uraemic syndrome. A case control study found a statistically significant association with the purchase of raw and/or ready-to-eat (RTE) food supplied by the implicated butchers’ shops. Isolates of STEC O157 were detected in two raw lamb burgers taken from one of the butchers’ premises. Subsequent environmental sampling identified STEC O157 in bovine faecal samples on the farm supplying cattle to the implicated butchers for slaughter. Whole genome sequencing (WGS) was performed on the Illumina HiSeq 2500 platform on all cultures isolated from humans, food and cattle during the investigation. Quality trimmed Illumina reads were mapped to the STEC O157 reference genome Sakai using bwa-mem, and single nucleotide polymorphisms (SNPs) were identified using gatk2. Analysis of the core genome SNP positions (>90 % consensus, minimum depth 10×, mapping quality (MQ)≥30) revealed that all isolates from humans, food and cattle differed by two SNPs. WGS analysis provided forensic-level microbiological evidence to support the epidemiological links between the farm, the butchers’ premises and the clinical cases. Cross-contamination from raw meat to RTE foods at the butchers’ premises was the most plausible transmission route. The evidence presented here highlights the importance of taking measures to mitigate the risks of cross-contamination in this setting.

show abstract

Section: Discussionmentioning

confidence: 99%

Farm-to-fork investigation of an outbreak of Shiga toxin-producing Escherichia coli O157

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Liquid cultures of Stx2 producing EHEC strain 86-24 16 and Stx1 expressing isolate EDL 973 17 were grown in 5 liters of Luria-Bertani medium. After 4 hours of incubation, 0.4 mg/L mitomycin C (Sigma-Aldrich, Taufkirchen, Germany) was added to enhance Stx release from the bacteria, followed by 20 hours of incubation.…”

Section: Stx Purificationmentioning

confidence: 99%

Molecular and functional analysis of Shiga toxin–induced response patterns in human vascular endothelial cells

Matussek

Lauber

Bergau

et al. 2003

Blood

101

View full text Add to dashboard Cite

Enterohemorrhagic Escherichia coli (EHEC) is the major cause of hemolyticuremic syndrome (HUS) characterized by microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. EHEC produces one or more Shiga toxins (Stx1 and Stx2), and it was assumed that Stx's only relevant biologic activity was cell destruction through inhibition of protein synthesis. However, recent data indicate that in vivo the cytokine milieu may determine whether endothelial cells survive or undergo apoptosis/necrosis when exposed to Stxs. In this study, we analyzed the genome-wide expression patterns of human endothelial cells stimulated with subinhibitory concentrations of Stxs in order to characterize the genomic expression program involved in the vascular pathology of HUS. We found that Stxs elicited few, but reproducible, changes in gene expression. The majority of genes reported in this study encodes for chemokines and cytokines, which might contribute to the multifaceted inflammatory response of host endothelial cells observed in patients suffering from EHEC disease. In addition, our data provide for the first time molecular insights into the epidemiologically well-established higher pathogenicity of Stx2 over Stx1. (Blood. 2003;102: 1323-1332

show abstract

“…An E . coli outbreak in Scotland infected 260 people and caused 17 fatalities , . The largest recall of food product contaminated with E .…”

Section: Introductionmentioning

confidence: 99%

Boron Doped Diamond Biosensor for Detection of Escherichia coli

Majid

Male

Luong

2008

J. Agric. Food Chem.

View full text Add to dashboard Cite

o-Nitrophenol, released from o-nitrophenyl--D-galactopyranose as catalyzed by -galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl--D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50°C. A biphasic calibration plot was observed: 4 × 10 4 to 2 × 10 5 cells/mL and 2 × 10 5 to 6 × 10 6 cells/mL for the first and second region, respectively. The detection limit was 4 × 10 4 cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by ∼40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.

show abstract

Escherichia coli 0157: outbreak in central Scotland

Cited by 10 publications

References 0 publications

Farm-to-fork investigation of an outbreak of Shiga toxin-producing Escherichia coli O157

Farm-to-fork investigation of an outbreak of Shiga toxin-producing Escherichia coli O157

Molecular and functional analysis of Shiga toxin–induced response patterns in human vascular endothelial cells

Boron Doped Diamond Biosensor for Detection of Escherichia coli

Contact Info

Product

Resources

About