Escherichia coli bacteria reduce graphene oxide to bactericidal graphene in a self-limiting manner

Akhavan, Omid; Ghaderi, Elham

doi:10.1016/j.carbon.2011.12.035

Cited by 526 publications

(327 citation statements)

References 54 publications

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“…The intensity ratio of the two peaks (I D /I G ) is a measure of disorder degree and is inversely proportional to the average size of the sp 2 clusters. 35,76 Here, we found that the I D /I G ratio for RES-rGO increased compared to that for GO (2.1 vs 1.70, respectively), suggesting the formation of new graphitic domains and the increase in the number of sp 2 clusters, 74 confirming that GO was successfully reduced by resveratrol.…”

supporting

confidence: 52%

“…32 Recently, biological molecules have been used to reduce GO; thus, Salas et al 33 have reported "green" reduction of GO via bacterial respiration. 33 Several microorganisms have been shown to reduce GO, including Shewanella spp., 34 Escherichia coli, 35,36 Pseudomonas aeruginosa, 13 Bacillus marisflavi, 36 and Ganoderma spp. 9 A number of studies have also reported GO reduction using various biomolecules such as ascorbic acid, 37 amino acids, 38 glucose, 39,40 bovine serum albumin, 41 melatonin, 42 and humanin.…”

mentioning

confidence: 99%

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Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Gurunathan¹,

Han²,

Kim³

et al. 2015

IJN

158

129

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Objective Graphene represents a monolayer or a few layers of sp 2 -bonded carbon atoms with a honeycomb lattice structure. Unique physical, chemical, and biological properties of graphene have attracted great interest in various fields including electronics, energy, material industry, and medicine, where it is used for tissue engineering and scaffolding, drug delivery, and as an antibacterial and anticancer agent. However, graphene cytotoxicity for ovarian cancer cells is still not fully investigated. The objective of this study was to synthesize graphene using a natural polyphenol compound resveratrol and to investigate its toxicity for ovarian cancer cells. Methods The successful reduction of graphene oxide (GO) to graphene was confirmed by UV-vis and Fourier transform infrared spectroscopy. Dynamic light scattering and scanning electron microscopy were employed to evaluate particle size and surface morphology of GO and resveratrol-reduced GO (RES-rGO). Raman spectroscopy was used to determine the removal of oxygen-containing functional groups from GO surface and to ensure the formation of graphene. We also performed a comprehensive analysis of GO and RES-rGO cytotoxicity by examining the morphology, viability, membrane integrity, activation of caspase-3, apoptosis, and alkaline phosphatase activity of ovarian cancer cells. Results The results also show that resveratrol effectively reduced GO to graphene and the properties of RES-rGO nanosheets were comparable to those of chemically reduced graphene. Biological experiments showed that GO and RES-rGO caused a dose-dependent membrane leakage and oxidative stress in cancer cells, and reduced their viability via apoptosis confirmed by the upregulation of apoptosis executioner caspase-3. Conclusion Our data demonstrate a single, simple green approach for the synthesis of highly water-dispersible functionalized graphene nanosheets, suggesting a possibility of replacing toxic hydrazine by a natural and safe phenolic compound resveratrol, which has similar efficacy in the reduction of GO to rGO. Resveratrol-based GO reduction would facilitate large-scale production of graphene-based materials for the emerging graphene-based technologies and biomedical applications.

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supporting

confidence: 52%

mentioning

confidence: 99%

Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Gurunathan¹,

Han²,

Kim³

et al. 2015

IJN

158

129

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“…GO was prepared from natural graphite flakes by a modified Hummers method (Akhavan and Ghaderi 2012;Shahriary and Athawale 2014;Zheng et al, 2013). Briefly, graphite (3.0 g) was added to concentrated H 2 SO 4 (70 mL) under stirring at room temperature.…”

Section: Preparation Of Graphene Oxide (Go)mentioning

confidence: 99%

Graphene Oxide as Antimicrobial Against Two Gram-Positive and Two Gram-Negative Bacteria in Addition to One Fungus

Al‐Thani¹,

Patan²,

Al-Maadeed³

2014

OnLine Journal of Biological Sciences

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Graphene based materials have wide potential applications in biology, biomedical, agriculture environmental and biotechnology. Graphene Oxide (GO) is one of those materials and has a promising substance as antimicrobial agents. GO in this study was prepared by a modified Hummers method and was characterized by different techniques for confirmation of formation of GO. To study the antimicrobial activities of GO, it was tested against these microorganisms, one eukaryotic fungus (Candida albicans, C. albicans) two Gram negative bacteria (Escherichia coli (E. coli) ATCC 41570 and Pseudomonas aeruginosa (P. aeruginosa) ATCC 25619) and two Gram positive bacteria (Streptococcus faecalis (S. faecalis) ATCC 19433 and Staphylococcus aureus (S. aureus) ATCC 11632). Anti-microbial activity of GO was detected by spectrophotometer as indirect method to measure the growth and viable cell count as direct method. Readings were taken at successive incubated times. Results revealed that GO has antibacterial and anti-fungal activity against microorganisms used in this study. In conculosion the developed GO exhibit excellent antimicrobial property and GO affects more on Gram positive bacteria than Gram negative bacteria and fungi.

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“…These studies have shown the use of graphene in microbial fuel cells, 249 graphene as a scaffold for bacterial growth, 228 as well as the use of bacteria as a graphene oxide reducing agent. 250,251 Various in vitro toxicity studies have been conducted to determine the toxicity of graphene towards cancer [234][235][236][237][238] and human cell lines. 229,239 The cytotoxicity towards the cancer cell lines A549 was shown to depend largely on the cell culture medium components.…”

Section: Environmental and Biological Toxicity Of Graphenementioning

confidence: 99%

“…Li et al showed that mice hippocampal neurons could be grown on CVD graphene, with the scaffold even promoting neutrite growth. 230 Additional studies have shown that graphene can also be used as a scaffold for human colorectal adenocarcinoma HT-29 cells 251 and human stem cells. 229,231 Mammalian in vivo studies of the toxicity of graphene compounds have been conducted in mice.…”

Section: Environmental and Biological Toxicity Of Graphenementioning

confidence: 99%

Environmental applications using graphene composites: water remediation and gas adsorption

et al. 2013

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This review deals with wide-ranging environmental studies of graphene-based materials on the adsorption of hazardous materials and photocatalytic degradation of pollutants for water remediation and the physisorption, chemisorption, reactive adsorption, and separation for gas storage. The environmental and biological toxicity of graphene, which is an important issue if graphene composites are to be applied in environmental remediation, is also addressed.

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Escherichia coli bacteria reduce graphene oxide to bactericidal graphene in a self-limiting manner

Cited by 526 publications

References 54 publications

Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Reduction of graphene oxide by resveratrol: a novel and simple biological method for the synthesis of an effective anticancer nanotherapeutic molecule

Graphene Oxide as Antimicrobial Against Two Gram-Positive and Two Gram-Negative Bacteria in Addition to One Fungus

Environmental applications using graphene composites: water remediation and gas adsorption

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