Escherichia coli growth and hydrogen production in batch culture upon formate alone and with glycerol co-fermentation at different pHs

Optimizing strategy for Escherichia coli growth and hydrogen production during glycerol fermentation in batch culture: Effects of some heavy metal ions and their mixtures

“…The similar value for H 2 yield by E. coli during glycerol fermentation has been reported previously [35]. In spite of glycerol being very cheap substrate for fermentation, the value of H 2 yield by E. coli with glycerol fermentation reported by different groups [3,4,35] is not high enough to be employed in H 2 production biotechnology [2]; that is why the significant increase in H 2 yield by optimization of the technology conditions should be required. H 2 yield was markedly stimulated (1.9-3-fold) in the presence of some heavy metal ion mixtures of 0.02-0.1 mM Ni 2+ + Fe 3+ , Ni 2+ + Fe 3+ + Mo 6+ and Fe 3+ + Mo 6+ Mo 6 (see Table 1).…”

Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives

“…Thus, glucose and glycerol concentrations might have significant role in H 2 production rate changing. À Formate added into the medium with glycerol stimulated E. coli growth and H 2 production at different pHs [75,76]. Interestingly, formate (10 mM) recovered H 2 production of Hyd-2 (hybC) or Hyd-4 (hyfG) mutants depending on pH [75,76].…”

“…À Formate added into the medium with glycerol stimulated E. coli growth and H 2 production at different pHs [75,76]. Interestingly, formate (10 mM) recovered H 2 production of Hyd-2 (hybC) or Hyd-4 (hyfG) mutants depending on pH [75,76]. These results highlight the key role of Hyd-3 at both pH 6.5 and pH 7.5, as well as the role of Hyd-2 and Hyd-4 at pH 7.5 for H 2 production by E. coli during glycerol and formate co-fermentation.…”

Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium,Vibrio tritonius

“…To date, the majority of studies on fermentative H 2 production have been on strict anaerobes of Clostridium (Ferchichi et al, 2005; Jayasinghearachchi et al, 2010; Jo, Lee & Park, 2008b; Junghare, Subudhi & Lal, 2012), and facultative anaerobes such as Escherichia coli (Maeda, Sanchez-Torres & Wood, 2012; Trchounian & Trchounian, 2014; Trchounian et al, 2015) and Enterobacter aerogenes (Jo et al, 2008a; Zhao et al, 2009; Zhao et al, 2001). These terrestrial bacteria were not suitable biocatalysts in fermentative H 2 production under saline conditions due to sensitivity to salts in H 2 production (Alshiyab et al, 2008).…”