Escherichia coli producing VIM-1 carbapenemase isolated on a pig farm

Fischer, Jennie; Rodríguez, Irene; Schmoger, Silvia; Friese, Anika; Roesler, Uwe; Helmuth, Reiner; Guerra, Beatriz

doi:10.1093/jac/dks108

Cited by 138 publications

(114 citation statements)

References 9 publications

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“…Reports around the world show that P. aeruginosa is notorious for its resistance to antibiotics [16][17][18]. Our result on the antimicrobial susceptibility patterns of P. aeruginosa isolates to antibiotics is similar to the work of Franco et al [19], in which high-level resistance of P. aeruginosa isolates to the carbapenems was reported in Brazil. Our result is also similar to the work of Aibinu et al [20] & Olutayo and Abimbola [21] -who reported similar resistance patterns of P. aeruginosa isolates in southwest Nigeria.…”

Section: Discussionsupporting

confidence: 91%

“…Nevertheless, the timely and accurate detection of organisms harbouring genes responsible for the production of AmpC β-lactamases is critical for total patient care. The occurrence of AmpC-producing P. aeruginosa from abattoir calls for effective monitoring and possibly a ban on the use of antibiotics in the production and/or rearing of food producing animals in order to assuage the emergence and spread of drug resistant bacteria in the community [7,19,21]. Thus, this study presumptively detected the occurrence of AmpC-producing P. aeruginosa isolates from feacal matter of cow in a local abattoir.…”

Section: Introductionmentioning

confidence: 97%

“…Thus, molecular characterization of the genes that encode AmpC β-lactamase production in this organism is crucial for a reliable epidemiological investigation into the possible emergence and dissemination of AmpC positive bacteria in the community. monitoring and possibly a ban on the use of antibiotics in the production and/or rearing of food producing animals in order to assuage the emergence and spread of drug resistant bacteria in the community [7,19,21]. Thus, this study presumptively detected the occurrence of AmpC-producing P. aeruginosa isolates from feacal matter of cow in a local abattoir.…”

mentioning

confidence: 99%

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Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Chika¹

2017

JMEN

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The possible contamination of food and/or food-producing animals with multidrug resistant bacteria including AmpC-producing Pseudomonas aeruginosa is considered a potential source for the wide dissemination of AmpC β-lactamase in the community. This portends public health risks for the populace -owing to the multidrug resistant nature of these organisms. There is paucity of data on the prevalence of AmpCproducing bacteria in Abakaliki, Nigeria -which was why this study was carried out. A total of 40 feacal swab samples from cow dung in a local abattoir were bacteriologically examined for the isolation and antimicrobial susceptibility testing of P. aeruginosa isolates using standard microbiological procedures on cetrimide selective agar and Kirby-Bauer disk diffusion method respectively. AmpC β-lactamase was phenotypically confirmed using the ceftazidime-imipenem antagonism test (CIAT). A total of 12 P. aeruginosa isolates were recovered from the samples; and they showed varied levels of resistance to the tested antibiotics especially to cefoxitin, ertapenem, oxacillin, amikacin and cefotaxime. Among the 12 P. aeruginosa isolates, AmpC β-lactamase was phenotypically detected in 3 (25%) isolates by the CIAT method. This study has presumptively shown that AmpC β-lactamase-producing P. aeruginosa isolates occur in abattoir. Thus, molecular characterization of the genes that encode AmpC β-lactamase production in this organism is crucial for a reliable epidemiological investigation into the possible emergence and dissemination of AmpC positive bacteria in the community. monitoring and possibly a ban on the use of antibiotics in the production and/or rearing of food producing animals in order to assuage the emergence and spread of drug resistant bacteria in the community [7,19,21]. Thus, this study presumptively detected the occurrence of AmpC-producing P. aeruginosa isolates from feacal matter of cow in a local abattoir. Keywords: Materials and Methods Sample collection and processingA total of forty (40) swab samples were collected from feacal matter of cow in a local abattoir using sterile swab sticks. After collection, each of the swab sticks was returned into their respective containers, labeled and transported to the Microbiology Laboratory Unit of Ebonyi State University, Abakaliki for bacteriological analysis. Isolation and identification of Pseudomonas aeruginosaCetrimide selective agar (Oxoid, UK) containing 10 ml of glycerol was used for the selective isolation of P. aeruginosa from the test samples. Each of the feacal swab samples was inoculated in 5 ml double strength of freshly prepared nutrient broth (Oxoid, UK) and incubated for 18-24 hours at 30 o C. A loopful of the broth culture was aseptically streaked on cetrimide selective agar plates; and the plates were incubated at 30 o C 18-24 hours. Suspected colonies were inoculated onto freshly prepared cetrimide selective agar for the isolation of pure cultures of P. aeruginosa. P. aeruginosa produces greenish/bluish pigmentation on cetrimide selec...

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 97%

mentioning

confidence: 99%

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Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Chika¹

2017

JMEN

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“…Additionally, plasmid-mediated AmpC beta-lactamases and, even more alarming, carbapenemases are leading to a worsening of the situation in both human and veterinary medicine (13). Although the resistant bacteria were initially observed in human samples, an increase in the detection of ESBL/AmpC-producing E. coli in animals, such as pigs (9,14), horses (15), cattle, dogs, cats (4,15), fish (16), and, particularly, broiler chickens, has been reported worldwide (3,8,12,14).…”

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confidence: 99%

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Laube¹,

Friese²,

Salviati³

et al. 2013

Appl Environ Microbiol

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133

106

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Antimicrobial resistance of Escherichia coli to modern beta-lactam antibiotics due to the production of extended-spectrum betalactamases (ESBL) and/or plasmid-mediated AmpC beta-lactamases (AmpC) represents an emerging and increasing resistance problem that dramatically limits therapeutic options in both human and veterinary medicine. The presence of ESBL/AmpC genes in commensal E. coli from food-producing animals like broilers may pose a human health hazard. However, there are no data available concerning the prevalence of ESBL/AmpC-producing E. coli in German broiler flocks using selective methods. In this longitudinal study, samples were taken from seven conventional broiler fattening farms at three different times within one fattening period. Various samples originating from the animals as well as from their direct environment in the barn were investigated for the occurrence of ESBL/AmpC-producing E. coli. Average detection levels of 51, 75, and 76% in animal samples collected during the three samplings in the course of the fattening period demonstrate a colonization of even 1-day-old chicks, as well as a continuous significant (P < 0.001) increase in prevalence thereafter. The detection frequencies in housing environmental samples were relatively high, with an increase over time, and ranged between 54.2 and 100%. A total of 359 E. coli isolates were characterized by PCR and partly via the disc diffusion method. This study shows that prevalence of ESBL/AmpC-producing E. coli increases during the fattening period of the broiler flocks examined. Both colonized day-old chicks and contaminated farm environments could represent significant sources of ESBL/AmpC-producing E. coli in German broiler fattening farms.

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“…Apart from the occurrence of ESBL producers, some recent studies identified carbapenemases in Gram-negative bacteria from animals, i.e., OXA-23-producing Acinetobacter genomospecies 15TU from dairy cattle in France (9), OXA-23-producing Acinetobacter spp. from horses in Belgium (10), and VIM-1-producing E. coli isolates from pigs and poultry in Germany (11,12). Our study aimed to evaluate the occurrence of ESBL-producing or carbapenemase-producing Enterobacteriaceae as commensals (rectal isolates) or as pathogens (urinary tract infections) among companion animals living in France.…”

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confidence: 99%

Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

Poirel

Nordmann

Ducroz

et al. 2013

Antimicrob Agents Chemother

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Screening of extended-spectrum ␤-lactamase (ESBL)-producing Gram-negative bacteria in companion animals living in the Paris area in France identified a high rate of CTX-M-15-producing Klebsiella pneumoniae. Those isolates were recovered during the 2010-2011 period from both infections and asymptomatic colonizations. Sequence typing revealed that most of these isolates belonged to sequence type ST274. Interestingly, the bla CTX-M-15 gene was located on a specific and novel plasmid scaffold. These findings highlight that companion animals may be reservoirs for CTX-M-15-producing K. pneumoniae evolving separately from the human reservoir of CTX-M-15 producers.

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Escherichia coli producing VIM-1 carbapenemase isolated on a pig farm

Cited by 138 publications

References 9 publications

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Prevalence of AmpC β -Lactamase-Producing Pseudomonas aeruginosa Isolates From Feacal Matter of Cow

Longitudinal Monitoring of Extended-Spectrum-Beta-Lactamase/AmpC-Producing Escherichia coli at German Broiler Chicken Fattening Farms

Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

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