Escherichia coli variants for gas and indole production at elevated incubation temperatures

Bueschkens, Donna H.; Stiles, Michael E.

doi:10.1128/aem.48.3.601-605.1984

Cited by 27 publications

(19 citation statements)

References 27 publications

(28 reference statements)

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“…All E. coli strains were gas producing. These results are similar to the characteristics exhibited by strains by other researchers [29]. The difference may be result of E. coli isolates from different origin or genetic descendent from Shigella.…”

Section: Biochemical Tests Of E Colisupporting

confidence: 90%

Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets

Alam¹,

Khalil²,

Ayub³

et al. 2013

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Nutritional value of vegetables and high prices of meat and meat originated food compel common people to consume plant originated food particularly salad vegetables. Microbial population of vegetable surfaces contains a large number of pathogenic bacteria including members of Enterobactereace like Escherichia coli (E. coli). A survey was conducted in three major markets of Rawalpindi, Pakistan. Tomato, lettuce, cabbage and cucumber samples were collected from three shops of each market. Each vegetable was analysed as unwashed and washed for total coliforms, faecal coliforms and E. coli by FAO (Food Quality Manual). About two hundred and fifty E. coli isolates were preserved, serotyped for presence of O157 serotype. Total coliforms, faecal coliforms and E. coli count exceeded the permissible limits in most samples. The highest Total coliforms were associated with cabbage (3.78 log 10 cfu/g). Cucumber was the least contaminated by Total coliforms (2.15 log 10 cfu/g). E. coli was detected in tomato, lettuce, cucumber and cabbage. Washed samples showed reduced bacterial population. Seventy six isolates of E. coli were biochemically characterized and serotyped for O157 antigen. A majority of strains could not be identified by serotyping. These findings conclude with high potentially pathogenic microbial load on salad vegetables and urge for preventive action on priority basis.

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Section: Biochemical Tests Of E Colisupporting

confidence: 90%

Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets

Alam¹,

Khalil²,

Ayub³

et al. 2013

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“…However, the relationship between lipid profiles and CVD risks in CKD patients remains uncertain. In dialysis patients, serum LDL-c level has a negative association with all-cause mortality [21,22], the phenomenon of which is called 'reverse epidemiology'. Low serum HDL-c levels are common among patients with CKD and ESRD [23][24][25].…”

Section: Discussionmentioning

confidence: 99%

“…Indoxyl sulfate (IS), a protein-bound uremic toxin, is one of the organic anions synthesized in the liver from indole [2]. CYP2E1 is the major isoform of P450 enzymes responsible for microsomal oxidation of indole to indoxyl [3].…”

Section: Introductionmentioning

confidence: 99%

Indoxyl Sulfate and High-density Lipoprotein Cholesterol in Early Stages of Chronic Kidney Disease

Wang

Fei

et al. 2019

Preprint

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Background: High indoxyl sulfate (IS) levels and low high-density lipoprotein cholesterol (HDL-c) levels are both risk factors of cardiovascular diseases (CVD) in chronic kidney disease (CKD) patients, the connection between which has not been clearly clarified. This study aimed to explore the relationship between IS and HDL-c levels in early stages of CKD population. Methods: Patients of CKD stage 1-3 were enrolled in this cross-sectional study. Correlations between HDL-c and IS were investigated among various clinicopathological variables.Results: A total of 205 CKD patients (96 men) with a mean age of 43.3 years old were included in this research. There were 96 patients (46 men) in CKD stage1 and 109 (50 men) in CKD stage 2 or stage 3. IS levels were significantly higher in CKD 2+3 group (1.50±1.74μg/ml vs 0.94±0.66μg/ml, p=0.007), while HCL-c levels were lower (1.19±0.39mmol/L vs 1.33±0.45 mmol/L, p=0.017) compared to CKD 1 group. Among all the patients, a negative correlation was observed between IS and HDL-c levels (r=-0.244, p=0.001). IS level was an independent risk factor for low HDL-c (<1.04mmol/L) incidence even after controlling for potential confounders (OR=1.63, 95% CI: 1.11-2.39, p=0.013). IS and HDL-c were both risk factors for predicting CKD stage 3. Conclusions: Metabolic disorder of HDL-c occurs in early CKD stages, probably attributed by increased IS level. Early management of dyslipidemia and uremic toxin retention is important for delaying disease progression and preventing cardiovascular events. Keywords: Indoxyl sulfate, High-density lipoprotein cholesterol, Chronic kidney disease, Cardiovascular disease, Lipids

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“…The high frequency of gas and indole positive strains among unidentified strains requires further taxonomic studies. It is possible that prolonged immersion of fecal bacteria in natural waters alters their biochemical properties (Bueschkens and Stiles, 1984).…”

Section: Discussionmentioning

confidence: 99%

Annual variation and reliability of fecal indicators in a polluted river

Niemi

1988

Environ. Toxicol. Water Qual.

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Fecal indicators were enumerated over a period of one year in the river Vantaanjoki discharging to the Gulf of Finland. The river was divided into three zones, one affected by nonpoint loading, another receiving treated domestic sewage, and the lower reach with no substantial further loading. The reliability of routine enumeration methods for thermotolerant coliforms and fecal streptococci was tested by isolating strains for confirmation. Fecal indicators showed wide spatial and temporal variation: the concentrations and numbers were low in summer, higher in winter under the ice cover, and highest during the periods of high discharge in spring and autumn. Of the 931 thermotolerant coliforms isolated, 74% were identified as Escherichia coli by the API 20 E identification system. A total of 3441 strains were tested for gas and indole production at 44.5°C. Of these, 73% were gas positive and 89% indole positive. Of the 3567 fecal streptococci, 67% were confirmed as catalase negative and esculin positive on bile esculin azide agar at 44.5°C. The proportion of confirmed fecal indicators varied both in different reaches of the river and seasonally. The enumeration of fecal streptococci was in some cases unreliable. Correlations between different fecal indicators and between fecal indicators and chemical water quality variables were calculated within seasons. Correlation coefficients varied with season but showed good overall compatibility.

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Escherichia coli variants for gas and indole production at elevated incubation temperatures

Abstract: Departments of Mi(crobiology ancd Foods anid Nitr ition, Tl/e University ojA/bAlbert Edmontoo,. Alber-tti, Canadai T6G 2M8

Cited by 27 publications

References 27 publications

Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets

Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets

Indoxyl Sulfate and High-density Lipoprotein Cholesterol in Early Stages of Chronic Kidney Disease

Annual variation and reliability of fecal indicators in a polluted river

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Escherichia coli variants for gas and indole production at elevated incubation temperatures

Abstract: Departments of Mi(crobiology ancd Foods anid Nitr ition, Tl/e University ojA/bAlbert Edmontoo,. Alber-tti, Canadai T6G 2M8

Cited by 27 publications

References 27 publications

Prevelance of total coliforms, faecal coliforms and &lt;i&gt;E. coli&lt;/i&gt; in Rawalpindi vegetable markets

Prevelance of total coliforms, faecal coliforms and &lt;i&gt;E. coli&lt;/i&gt; in Rawalpindi vegetable markets

Indoxyl Sulfate and High-density Lipoprotein Cholesterol in Early Stages of Chronic Kidney Disease

Annual variation and reliability of fecal indicators in a polluted river

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Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets

Prevelance of total coliforms, faecal coliforms and <i>E. coli</i> in Rawalpindi vegetable markets