Escherichia fergusonii, an Underrated Repository for Antimicrobial Resistance in Food Animals

Tang, Biao; Chang, Jiang; Chen, Yifei; Lin, Jiahui; Xiao, Xingning; Xia, Xiaodong; Lin, Jun; Yang, Hua; Zhao, Guoping

doi:10.1128/spectrum.01617-21

Cited by 36 publications

(26 citation statements)

References 37 publications

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“…These critical AMR genes in E. coli isolates from food animals present a tremendous public health concern. It’s important to mention that the acquired AMR genes in bacterial genomes do not inevitably confer phenotypic resistance and vice versa ( Boolchandani et al, 2019 ; Tang et al, 2022a ). Other mechanisms such as SNPs and MDRtransporter also significantly contribute to the phenotypic resistance ( Boolchandani et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The recent emergence and rapid increase of multi-drug resistant (MDR, resistance to more than three kinds of antibiotics) bacteria have caused public concern, represented by Escherichia coli resistant to carbapenem, colistin and tigecycline which were recognized as the last line of resort ( Li et al, 2017 ; He et al, 2019 ; Ma et al, 2022 ). Due to incorrect use and misuse of antibiotics, the spread of antimicrobial resistance (AMR) is accelerating ( Wang et al, 2022 ; Tang et al, 2022a , b ). The continuous spread of AMR not only increases the difficulty of preventing and controlling livestock and poultry diseases but also seriously threatens livestock products’ safety and endangers consumers’ health ( Xu et al, 2022 ; Li et al, 2022a ).…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

et al. 2022

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Escherichia coli is considered an opportunistic pathogen and an indicator for antimicrobial resistance (AMR) monitoring. Despite many reports on its AMR monitoring, studies based on genome-based analysis of AMR genes are still insufficient. Here, 181 E. coli strains were isolated from anal swab samples collected from pigs and chickens of animal farms located in Eastern China and sequenced through the Illumina platform. The results showed that 87.85% (159/181) of the E. coli isolates were multidrug-resistant (MDR). Ampicillin (AMP)- spectinomycin (SPT)- tetracycline (TET)- florfenicol (FFC)- sulfisoxazole (SF)- trimethoprim/sulfamethoxazole (SXT) was the predominant AMR pattern. By whole-genome sequencing, we found that ST10 (10.49%, 19/181) and ST48 (7.18%, 13/181) were major sequence types. IncFIB and IncX1 were the most prevalent plasmid replicons. The AMR genes blaNDM-5 (1.10%, 2/181), mcr-1 (1.10%, 2/181), tet(X4) (1.10%, 2/181), and cfr (6.08%, 2/181) were also found in these isolates. In addition, among the 169 virulence genes detected, we identified astA (37.02%, 67/181), hlyA (1.66%, 3/181), hlyB (1.66%, 3/181) and hlyD (1.66%, 3/181), which were closely related to heat-stable enterotoxin 1 and α-hemolysin. In addition, there were 33 virulence genes associated with the iron uptake system, and 46 were adhesion-related genes. Our study highlighted the need for routine surveillance of AMR with advanced genomic approaches, providing up-to-date data on the prevalence of AMR for the development and execution of antimicrobial stewardship policy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

et al. 2022

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“…Among these last-resort antibiotics, colistin was heavily used in food-producing animal industries in China for a long period of time until it was banned as a growth promoter in 2017 ( Walsh and Wu, 2016 ). However, colistin-resistant Gram-negative pathogens, especially Enterobacteriaceae pathogens, still have high presence in poultry/livestock farm environments ( Liu et al, 2016 ; Tang et al, 2022 ), and the plasmid-borne resistance gene mcr-1 is the main factor contributing to widespread colistin resistance ( Arcilla et al, 2016 ). In addition, although the use of carbapenems, broad-spectrum β-lactam class antibiotics, is authorized only in clinics ( Ahmad et al, 2019 ; Farhat and Khan, 2020 ; Nasser et al, 2020 ), their main resistance gene, bla NDM-1 , is still frequently identified in Enterobacteriaceae pathogens in poultry/livestock farm environments.…”

Section: Discussionmentioning

confidence: 99%

Rapid detection of multiple resistance genes to last-resort antibiotics in Enterobacteriaceae pathogens by recombinase polymerase amplification combined with lateral flow dipstick

Wang

Pan³

et al. 2023

Front. Microbiol.

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The worrying emergence of multiple resistance genes to last-resort antibiotics in food animals and human populations throughout the food chain and relevant environments has been increasingly reported worldwide. Enterobacteriaceae pathogens are considered the most common reservoirs of such antibiotic resistance genes (ARGs). Thus, a rapid, efficient and accurate detection method to simultaneously screen and monitor such ARGs in Enterobacteriaceae pathogens has become an urgent need. Our study developed a recombinase polymerase amplification (RPA) assay combined with a lateral flow dipstick (LFD) for simultaneously detecting predominant resistance genes to last-resort antibiotics of Enterobacteriaceae pathogens, including mcr-1, blaNDM-1 and tet(X4). It is allowed to complete the entire process, including crude DNA extraction, amplification as well as reading, within 40 min at 37°C, and the detection limit is 101 copies/μl for mcr-1, blaNDM-1 and tet(X4). Sensitivity analysis showed obvious association of color signals with the template concentrations of mcr-1, blaNDM-1 and tet(X4) genes in Enterobacteriaceae pathogens using a test strip reader (R2 = 0.9881, R2 = 0.9745, and R2 = 0.9807, respectively), allowing for quantitative detection using multiplex RPA-LFD assays. Therefore, the RPA-LFD assay can suitably help to detect multiple resistance genes to last-resort antibiotics in foodborne pathogens and has potential applications in the field.

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“…As described in a previous study, the 11 E. coli strains were subjected to S1-PFGE to identify the number and size of plasmids [ 28 , 29 ]. The overnight cultures were washed with PBS buffer and then embedded in agarose plugs.…”

Section: Methodsmentioning

confidence: 99%

Genome Assessment of Carbapenem- and Colistin-Resistant Escherichia coli from Patients in a Sentinel Hospital in China

Tang

Lin

et al. 2022

Cells

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Antimicrobial-resistant (AMR) pathogens are a significant threat to public health worldwide. However, the primary carrier of AMR genes, particularly against last-resort antibiotics, is still only partially studied in Chinese hospitals. In a sentinel hospital in China, we collected 157 E. coli strains from patients between January and July 2021. One blaNDM-1-, nine blaNDM-5-, and one mcr-1-positive E. coli recovered from inpatients were identified as resistant to meropenem and colistin. There are 37 virulence genes discovered in the 11 strains, including astA in strain EC21Z-147 (O128: H4), which belongs to the enteroaggregative E. coli (EAEC). The blaNDM gene is distributed into distinct ST types, including ST48, ST616, ST410, ST711, and ST2003, while the mcr-1 gene was identified in ST117. The conjugative plasmids IncX3, IncI1-I, and IncI2 mediated the blaNDM-5 and mcr-1 genes detected among inpatients. Notably, the youngest age at which mcr-1-positive E. coli has been reported was at one day old, in a child in which the strain is closely related to strains with animal origins. Hospitals are major environments for the spread and dissemination of critical virulence and AMR genes, which requires active monitoring systems at the genome level to surveil the spread of virulence and AMR.

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Escherichia fergusonii, an Underrated Repository for Antimicrobial Resistance in Food Animals

Abstract: E. fergusonii , a member of the genus Escherichia , has been reported to transmit via the food chain and cause diseases in humans. However, the prevalence of multidrug-resistant E. fergusonii , especially mcr-1 -positive E. fergusonii isolates, has rarely been reported.

Cited by 36 publications

References 37 publications

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China

Rapid detection of multiple resistance genes to last-resort antibiotics in Enterobacteriaceae pathogens by recombinase polymerase amplification combined with lateral flow dipstick

Genome Assessment of Carbapenem- and Colistin-Resistant Escherichia coli from Patients in a Sentinel Hospital in China

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