Genome Assessment of Carbapenem- and Colistin-Resistant Escherichia coli from Patients in a Sentinel Hospital in China

Ma, Jian-Gang; Tang, Biao; Lin, Jiahui; Ed-Dra, Abdelaziz; Lin, Hui; Wu, Jing; Dong, Yuzhi; Yang, Hua; Yue, Min

doi:10.3390/cells11213480

Cited by 8 publications

(8 citation statements)

References 53 publications

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“…It has been reported that IncX3 plasmids carrying bla NDM-5 are highly similar in various countries and host sources [29]. This suggests that these plasmids have the potential to e ciently disseminate bla NDM-5 among humans, animals, food, and the environment, thereby contributing to the rapid spread of bla NDM-5harboring isolates [30]. Various studies have reported different prevalence rates of the mcr-1 gene in E. coli isolates, ranging from 0.25% (10/3902) in Italy [31] to 1% in China [32].…”

Section: Discussionmentioning

confidence: 99%

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Xie

Han

et al. 2023

Preprint

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Background: Genomic characterization of carbapenem-resistant Escherichia coli (CREC) isolates is necessary to understand its transmission and implement infection control measures, given its potential threat to public health. Method：A total of 100 CREC isolates collected from 2013–2021 at a Nanjing tertiary hospital were analyzed using whole-genome sequencing (WGS). The distribution of resistant determinants (RTs), virulence factors (VFs), multi-locus sequence type (MLST), and FimH types was determined. Furthermore, a phylogenetic tree was constructed to investigate the genetic relationships. Result:All CREC strains (n=100) carried at least one carbapenem hydrolyzing ß-lactamase (CHßL)-encoding gene, with blaNDM (n=83, 83.0%) being the most common. Multiple RTs were detected with blaCTX (n=83, 83.0%) and blaTEM (n=78, 78.0%) being the most dominant ones. MLST identified 37 distinct STs, with ST167 (n=18, 18.0%) and ST410 (n=13, 13.0%) being the most predominant clones. Multiple plasmid replicons were found. The strains displayed 64 different virulence factors, with terC (n=98, 98.0%) and traT (n=76, 76.0%) being the most frequent. Genetic diversity was observed between the 100 CREC isolates, with clonal dissemination of ST167, ST361, ST2179, ST410, and ST131 being observed. Conclusion: The prevalence of blaNDM-5, a major blaCHßLs, is concerning due to its distribution of various co-carried resistance genes and their potential to spread through multiple plasmid replicons. The isolates exhibit genetic diversity based on STs, Fim typing, serotyping, and phylogenetic tree analysis. ST167, ST361, ST2179, ST410, and ST131 are linked to clonal dissemination, highlighting the need for effective infection control measures to prevent their spread.

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Section: Discussionmentioning

confidence: 99%

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Xie

Han

et al. 2023

Preprint

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“…This result indicated that bacteria coharboring resistance genes and virulence factors are widespread, limiting antibiotic treatment. The astA gene has been identified in various DEC strains with outbreaks of diarrhea [ 66 , 67 , 68 , 69 ]. This study indicated that the astA gene might increase the strain’s virulence.…”

Section: Discussionmentioning

confidence: 99%

Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet

et al. 2023

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tet(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying tet(X4) are a severe threat to human health. However, information describing Escherichia coli coharboring tet(X4) with virulence genes is limited. Here, we isolated an E. coli strain coharboring tet(X4) and the heat-stable toxin gene astA from a dead piglet. The strain named 812A1-131 belongs to ST10. The genome was sequenced using the Nanopore and Illumina platforms. The virulence genes astA and tet(X4) are located on the chromosome and in the IncHI1-type plasmid p812A1-tetX4-193K, respectively. The plasmid could be conjugatively transferred to recipient E. coli J53 with high frequency. In vivo experiments showed that strain 812A1-131 is pathogenic to Galleria mellonella and could colonize the intestines of mice. In summary, pathogenic E. coli could receive a plasmid harboring the tet(X4) gene, which can increase the difficulty of treatment. The prevalence and transmission mechanisms of pathogenic bacteria coharboring the tet(X4) gene need more attention.

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“…S1-PFGE was used to detect plasmid size in strains performed as previously described ( Ma et al, 2022a , b ; Tang et al, 2022b ). In brief, the plugs were made from the fresh cultures embedded in 2% gold agarose and lysed with cell lysis buffer.…”

Section: Methodsmentioning

confidence: 99%

IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine

Wang

Yang

et al. 2023

Front. Microbiol.

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The tigecycline resistance gene tet(X4) was widespread in various bacteria. However, limited information about the plasmid harboring the tet(X4) gene spread among the different species is available. Here, we investigated the transmission mechanisms of the tet(X4) gene spread among bacteria in a pig farm. The tet(X4) positive Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Enterobacter hormaeche were identified in the same farm. The whole genome sequencing (WGS) analysis showed that the K. pneumoniae belonged to ST727 (n = 11) and ST3830 (n = 1), E. cloacae and E. hormaeche belonged to ST524 (n = 1) and ST1862 (n = 1). All tet(X4) genes were located on the IncHI1 plasmids that could be conjugatively transferred into the recipient E. coli C600 at 30°C. Moreover, a fusion plasmid was identified that the IncHI1 plasmid recombined with the IncN plasmid mediated by ISCR2 during the conjugation from strains B12L to C600 (pB12L-EC-1). The fusion plasmid also has been discovered in a K. pneumoniae (K1L) that could provide more opportunities to spread antimicrobial resistance genes. The tet(X4) plasmids in these bacteria are derived from the same plasmid with a similar structure. Moreover, all the IncHI1 plasmids harboring the tet(X4) gene in GenBank belonged to the pST17, the newly defined pMLST. The antimicrobial susceptibility testing was performed by broth microdilution method showing the transconjugants acquired the most antimicrobial resistance from the donor strains. Taken together, this report provides evidence that IncHI1/pST17 is an important carrier for the tet(X4) spread in Enterobacteriaceae species, and these transmission mechanisms may perform in the environment.

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Genome Assessment of Carbapenem- and Colistin-Resistant Escherichia coli from Patients in a Sentinel Hospital in China

Cited by 8 publications

References 53 publications

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Genomic characteristics of clinical carbapenem-resistant Escherichia coli isolates in a tertiary hospital, 2013–2021

Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet

IncHI1 plasmids mediated the tet(X4) gene spread in Enterobacteriaceae in porcine

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