ESCRT machinery and virus infection

Dai, Jun; Feng, Yiyi; Liao, Ying; Tan, Lei; Sun, Yingjie; Song, Cuiping; Qiu, Xusheng; Ding, Chan

doi:10.1016/j.antiviral.2023.105786

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2024

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Cited by 3 publications

References 201 publications

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Ubiquitination in viral entry and replication: Mechanisms and implications

Velez-Brochero,

Behera,

Afreen

et al. 2024

Advances in Virus Research

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Ubiquitination in viral entry and replication: Mechanisms and implications

Velez-Brochero,

Behera,

Afreen

et al. 2024

Advances in Virus Research

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Ultrasound Genomics Reveals a Signature for Predicting Breast Cancer Prognosis and Therapy Response

Li,

Chen,

Roche

et al. 2024

Cancer Biotherapy and Radiopharmaceuticals

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HRS Facilitates Newcastle Disease Virus Replication in Tumor Cells by Promoting Viral Budding

Chen,

Wang,

et al. 2024

IJMS

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Newcastle disease virus (NDV) is a highly pathogenic avian infectious disease agent and also a promising oncolytic virus with broad application prospects. The Endosomal Sorting Complex Required for Transport (ESCRT) machinery has been increasingly recognized for its crucial role in the life cycles of enveloped viruses, influencing processes such as viral entry, replication, and budding. In this study, we employed an RNA interference screening approach to identify key ESCRT components that regulate NDV replication in tumor cells. qPCR, immunofluorescence, and Western blot assays demonstrated that knockdown of HRS, CHMP4A, CHMP4B, and CHMP4C significantly impaired NDV replication in HeLa cells, with HRS exhibiting the most pronounced inhibitory effect. Additionally, HRS knockout significantly inhibited viral budding and suppressed NDV-induced cell death in HeLa cells. Notably, NDV infection was shown to significantly upregulate HRS gene and protein expression in a time-dependent manner. In conclusion, this study systematically identifies critical ESCRT components involved in NDV replication within tumor cells, with a particular focus on the role of HRS in promoting NDV’s replication by promoting viral budding, offering new insights for the development of NDV-based oncolytic therapies.

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ESCRT machinery and virus infection

Cited by 3 publications

References 201 publications

Ubiquitination in viral entry and replication: Mechanisms and implications

Ubiquitination in viral entry and replication: Mechanisms and implications

Ultrasound Genomics Reveals a Signature for Predicting Breast Cancer Prognosis and Therapy Response

HRS Facilitates Newcastle Disease Virus Replication in Tumor Cells by Promoting Viral Budding

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