Essential amino acid supplementation is associated with reduced serum C-reactive protein levels and improved circulating lymphocytes in post-acute inflamed elderly patients

Aquilani, Roberto; Zuccarelli, Ginetto Carlo; Maestri, Roberto; Boselli, Mirella; Dossena, Maurizia; Baldissarro, Eleonora; Boschi, Federica; Buonocore, Daniela; Verri, Manuela

doi:10.1177/20587384211036823

Cited by 8 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Others have also reported that the supplementation of EAAs reduced the inflammation rate in elderly patients. [ 61 ] In addition, dietary supplements of EAAs or whey proteins may also help in better functioning of the gut microflora which might improve health span and quality of life for older individuals.…”

Section: Discussionmentioning

confidence: 99%

Short‐Term Ingestion of Essential Amino Acid Based Nutritional Supplements or Whey Protein Improves the Physical Function of Older Adults Independently of Gut Microbiome

Azhar,

Verma,

Robeson

et al. 2024

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Scope: Dietary proteins and essential amino acids (EAAs) are the major nutritional supplements that support the growth and activity of gut microbes contributing to the wellbeing of their host. This study hypothesizes that daily supplementation of the diet with either EAAs or whey protein for 12 weeks would improve the gut microbiome of older adults.Methods and results: The stool samples are processed and subjected to Illumina‐based 16S ribosomal ribonucleic acid (rRNA) gene amplicon sequencing. In both groups, the most abundant families are found in order of relative abundance included: Bacteroidaceae, Lachnospiraceae, Ruminococcaceae, Prevotellaceae, Rikenellaceae, Enterobacteriaceae, Oscillospiraceae, Tannerellaceae, and Akkermansiaceae, which indicate that these subjects are able to maintain a same healthy microbial diversity in their guts. A significant finding is a reduction of proinflammatory cytokine, interleukin‐18 (IL‐18) in the EAAs group. It also uses the standard 6‐min walking test (6MWT) as a measure of cardiopulmonary fitness. At the end of the study, the subjects in the EAAs group perform significantly better in the 6MWT as compared to the whey group.Conclusion: It seems plausible that the improved physical performance and reduced proinflammatory cytokine, IL‐18 seen in the EAAs group, are independent of changes in gut microbiota.

show abstract

Section: Discussionmentioning

confidence: 99%

Short‐Term Ingestion of Essential Amino Acid Based Nutritional Supplements or Whey Protein Improves the Physical Function of Older Adults Independently of Gut Microbiome

Azhar,

Verma,

Robeson

et al. 2024

Molecular Nutrition Food Res

View full text Add to dashboard Cite

show abstract

“…In addition to their effects on TJ structures, EAAs may reduce intestinal inflammation, as AAs may exert anti-inflammatory effects [ 33 , 35 , 56 ]. In elderly diabetic patients, supplemented EAAs decrease serum TNF α levels [ 34 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Since TJ proteins have a fast turnover [ 31 , 32 ], they require adequate AA intake (in particular EAAs) in the intestinal lumen. Third, EAAs may exert an anti-inflammatory activity [ 33 , 34 , 35 ]. Fourth, in an experimental model of CKD (in mice), supplemented essential Branched-Chain AAs (BCAAs) have shown their capacity to re-equilibrate altered intestinal microbiota towards a development of healthier strains [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of a Metabolic Mixture on Gut Inflammation and Permeability in Elderly Patients with Chronic Kidney Disease: A Proof-of-Concept Study

et al. 2022

View full text Add to dashboard Cite

Intestinal barrier dysfunction is a risk factor for the progression of Chronic Kidney Disease (CKD). In this proof-of-concept study, we tested the effects of a mixture of Essential Amino Acids (EAAs) and mitochondrial substrates on intestinal inflammation and permeability of CKD patients. Eight patients with stage 3b-4 CKD and 11 healthy controls after overnight fasting underwent fecal measures of calprotectin and zonulin levels (indicators of gut inflammation and permeability, respectively) and determinations of plasma amino acids. Only CKD patients were supplemented with the mixture (8 g/d diluted in water). Compared to controls, baseline fecal calprotectin, zonulin and plasma levels of some AA in CKD patients were significantly higher (p = 0.005; p = 0.001 and p = 0.02 to 0.003, respectively). After six months of supplementation, CKD baseline fecal levels of calprotectin and zonulin significantly (borderline for zonulin) decreased (p = 0.008 and p = 0.05, respectively). Plasma AA concentrations, including glutamine and alanine, were higher than at the baseline (p: 0.05 to 0.008). The supplementation of this mixture was associated with improved intestinal barrier dysfunction. Increased plasma AA levels might contribute to the improvement of gut barrier dysfunction.

show abstract

“…Therefore, this fact explains the reduction of CRP levels when lysine and leucine were supplemented (79) and albumin regained its initial transport role.…”

Section: Albumin and Inflammatory Markersmentioning

confidence: 95%

Low-quality protein modulates inflammatory markers and the response to lipopolysaccharide insult: the case of lysine

et al. 2023

View full text Add to dashboard Cite

The relationship between non-communicable diseases (NCDs) and eating behaviour has long been attributed to a surplus of food and energy. However, the increase in the prevalence of NCDs and their underlying low-grade inflammatory milieu among people of low socioeconomic status (SES) has highlighted the existence of a confounding factor. In this work, we aim to study the effect of lysine deficiency on some inflammatory markers in the absence or presence of an inflammatory insult [lipopolysaccharide (LPS)]. For this purpose, thirty-two five-week-old male Sprague Dawley rats were randomly distributed into four groups: 1) control diet, 2) control diet+LPS, 3) lysine-deficient diet, and 4) lysine-deficient diet+LPS. Groups were only allowed their experimental diets for four weeks, during which LPS (50 µg/kg) or saline injections were administered intraperitoneally three times per week. The study showed that lysine deficiency blunted growth and body compartments development, decreased albumin production and elevated liver c-reactive protein (CRP) expression, independently of interleukins 6 and 1β, the main precursors of CRP. Also, the insufficient levels of lysine in the diet increased hyperactivity and triggered an anxiety-like behaviour, exacerbated with LPS. This work presents evidence that various physiological changes are associated with the absence of a sufficient amount of lysine in the diet and can potentially increase the risk factor for diseases. Thus, the increment in NCDs among the low SES populations, who heavily rely on cereals as a main source of protein, can be, at least partially, blamed on low lysine availability in diets.

show abstract

Essential amino acid supplementation is associated with reduced serum C-reactive protein levels and improved circulating lymphocytes in post-acute inflamed elderly patients

Cited by 8 publications

References 52 publications

Short‐Term Ingestion of Essential Amino Acid Based Nutritional Supplements or Whey Protein Improves the Physical Function of Older Adults Independently of Gut Microbiome

Short‐Term Ingestion of Essential Amino Acid Based Nutritional Supplements or Whey Protein Improves the Physical Function of Older Adults Independently of Gut Microbiome

Effects of a Metabolic Mixture on Gut Inflammation and Permeability in Elderly Patients with Chronic Kidney Disease: A Proof-of-Concept Study

Low-quality protein modulates inflammatory markers and the response to lipopolysaccharide insult: the case of lysine

Contact Info

Product

Resources

About