African locus beans (Parkia biglobosa) tree is a perennial legume tree that grows naturally in Africa and one of the trees the Forest Rangers enforced that farmers must preserve in their farmland because of its usefulness. Parkia biglobosa when fermented can be used to produce Iru and other useful products. This work employed the Particle Induced X-ray Emission (PIXE) in the determination of major, minor and trace element constituents of leaves, seeds, stem in Parkia biglobosa from Igbope, using a 2.5 MeV proton ion beam analysis (IBA) facility. The samples contain nineteen (19) elements namely; Na, Mg, Al, Si, P, S, Cl, K, Ca, Ti, Cr, Mn, Fe, Cu, Zn, Br, Sr, Y and Ba. Calcium has the highest average concentration 17183.2±25.8 ppm in the stem, followed by Sulphur 15361.7±20.0 ppm in the seed. The seed that is locally fermented to produce Iru has a higher concentration of Fe 1266.8±12.3 ppm. The results showed that Parkia biglobosa analysed in this work consist of several micro, major and trace elements of importance to human health. The plant is also free of harmful elements that can be hazardous to human health when consumed or used as herbal medicine.