Essential hyaluronan structure for binding with hyaluronan-binding protein (HABP) determined by glycotechnological approach

Programmable one cycle–one disaccharide unit modular synthesis of hyaluronan and chondroitin hybrid glycans

“…Previous studies have indicated that aggrecan could not recognize oligosaccharides with GlcAβ1-3GalNAc at the non-reducing terminal, 30 though in this work, the introduction of an amino group in the middle enhanced the binding of those glycans with aggrecan. For HA polysaccharides, the introduction of an amino group would weaken the interaction between the HA chain and its binding proteins.…”

“…These results indicated that the combination of the internal amino group and the C4-OH group of N-acetylhexosamine could generate a special glycan structure for the glycanprotein interaction, which is consistent with previous reports. 30,32 Previous studies have indicated that aggrecan could not recognize oligosaccharides with GlcAβ1-3GalNAc at the nonreducing terminal, 30 though in this work, the introduction of an amino group in the middle enhanced the binding of those glycans with aggrecan. For HA polysaccharides, the introduction of an amino group would weaken the interaction between the HA chain and its binding proteins.…”

“…These results indicated that the combination of the internal amino group and the C4–OH group of N -acetylhexosamine could generate a special 3D glycan structure for the glycan–protein interaction, which is consistent with previous reports. 30,32…”

“…According to the size of the HA-binding domain, hyaladherins are classified into types A, B, or C, which have different functions. 8,[28][29][30] For example, the HA receptor of endocytosis stabilin-2 (type A) is involved in sustaining proliferative signaling, the cell surface HA receptor CD44 (type B) is involved in the evasion of apoptosis, and the cartilage proteoglycan aggrecan (type C) is involved in the activation of invasion metastases. 31 Understanding the binding details of hyaladherins and HA may facilitate the elucidation of these biological processes.…”

Role of Hyaluronic Acid in Selected Malignant Neoplasms in Women

“…The biological activity of HA depends on attachment to hyaluronan binding proteins (HABPs) (Figure 2B). This group includes the proteins bound to the cell membrane (e.g., CD44, TLR2/4, HARE), as well as the proteins that are part of the extracellular matrix [5][6][7]. The main HA receptor is CD44 protein (cluster of differentiation 44), a single-chain trans-membrane glycoprotein (Figure 2B), which is also one of the most relevant markers of cancer stem cells (CSCs) in many types of cancer [8][9][10].…”