Essential Oil Composition, Antimicrobial and Cytotoxic Activities of Two Endemic <i>Stachys Cretica</i> Subspecies (Lamiaceae) from Turkey

Şerbetçi, Tuba; Demırcı, Betül; Güzel, Çağrla Bozkurt; Kültür, Şükran; Ergüven, Mıne; Başer, Kemal Hüsnü Can

doi:10.1177/1934578x1000500907

Cited by 12 publications

(8 citation statements)

References 31 publications

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“…Cytotoxicity of different extracts of Stachys species in human cancer cell lines reported in the literature are given in Table 2 for the sake of comparison with the toxicity of S. pilifera . It can be concluded from Table 2 that the alkaloid and terpenoid fractions of S. pilifera have a lower IC 50 and a higher efficiency than those observed with the extracts of Stachys species in human cancer cell lines( 33 34 35 ).…”

Section: Discussionmentioning

confidence: 92%

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

et al. 2018

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Stachys pilifera (S. pilifera) Benth (Lamiaceae) is used in traditional medicine to treat a variety of diseases. Despite some reports on the antitumor effects of some species of this genus, anticancer activity of S. pilifera has not been yet reported. Here, we examined the cytotoxic effect and cell death mechanisms of methanolic extract of S. pilifera and its alkaloid and terpenoid fractions on the HT-29 colorectal cell line. HT-29 cells were cultivated and then incubated in the methanolic extract of S. pilifera and its fractions at various concentrations for 24 h. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphology of cells was evaluated by contrast microscopy. Furthermore, effects of the tested extract and fractions were tested on some regulators of cell death and proliferation such as caspase-8, caspase-9, nuclear factor-κB (NF-κB), and nitric oxide (NO). Cisplatin was used as positive control. The estimated IC50 values of the methanolic extract, alkaloid and terpenoid fractions, and cisplatin against HT29 cell after 24 h were determined to be 612, 48.12, 46.44, and 4.02 μg/mL, respectively. Morphological changes such as plasma membrane blebbing, cell size reduction, and apoptotic bodies were observed in cells faced with the extract and fractions. S. pilifera extract and its fractions induced apoptosis through inhibition of NF-κB, NO, and activation of caspase-8 and caspase-9. Data showed considerable cytotoxic and antiproliferative effects of S. plifera on colorectal cell line through induction of apoptosis. These findings provide a basis for the therapeutic potential of S. pilfera in the treatment of colon cancer.

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Section: Discussionmentioning

confidence: 92%

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

et al. 2018

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“…Moreover, some species are reported in ethnomedicine as anti-fever, antidiarroheal, and for treatment of weaknesses of the heart and liver, and have shown a variety of activities (anti-allergic, anti-anoxic, antibacterial, anxiolytic, anti-inflammatory, antinephritic, antioxidant, antihepatitis and choleretic), confirming a wide variety of ethnomedicinal tradition [17]. In literature, the cytotoxic activity of different Stachys species and their essential oils against several cancer cell lines has been reported [18][19][20].…”

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confidence: 87%

Chemical Composition and In vitro Cytotoxic Activity of the Essential Oils of Stachys rupestris and Salvia heldreichiana, Two Endemic Plants of Turkey

Erdoğan

Everest

Martino

et al. 2013

Natural Product Communications

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The chemical composition of the essential oils of two endemic plants of Turkey, Stachys rupestris Montbret et Aucher ex Benth. and Salvia heldreichiana Boiss. ex Benth., were obtained by hydrodistillation and studied by GC and GC–MS. In all, 46 compounds were identified, 22 for S. rupestris accounting for 94.6 % of the total oil and 30 for S. heldreichiana, accounting for 91.9 % of the total oil. The presence of diterpenoids (50.7%) characterized the oil from S. rupestris, while S. heldreichiana oil was rich in oxygenated sesquiterpenes (78.9%). The essential oils were evaluated for their in vitro potential cytotoxic activity on three human cancer cell lines. The oil of S. rupestris showed the higher antiproliferative activity against PC-3 and MCF-7 cancer cell lines.

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“…The GC/MS and GC-FID analyses were carried out under experimental conditions similar to those reported earlier [5]. Identification of the EO components was carried out by comparison of their relative retention times with those of authentic samples, computer matching against commercial (Wiley GC/MS Library, MassFinder 3 Library) [14,15] and inhouse "Başer Library of Essential Oil Constituents", as well as MS literature data [16,17].…”

Section: Gas Chromatography-mass Spectrometry (Gc/ms) and Gas Chromatmentioning

confidence: 99%

Antimicrobial and Antioxidant Activities of Stachys lavandulifolia subsp. lavandulifolia Essential Oil and its Infusion

İşcan

Demırcı

Demirci

et al. 2012

Natural Product Communications

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Stachys lavandulifolia Vahl. subsp. lavandulifolia (Lamiaceae) is widely used in south Anatolia as a herbal tea. It is used for the treatment of gastrointestinal and respiratory disorders. Constituents of the essential oil obtained by hydrodistillation were analyzed both by GC-FID and GC/MS, simultaneously. Thirtyseven compounds representing 98.3% of the oil were characterized. β-Phellandrene (27%), α-pinene (18.5%) and germacrene-D (13%) were found as major components of the oil. Anticandidal, antibacterial and antioxidant properties of the oil and the prepared infusion were evaluated using several methods. The oil exhibited good inhibitory activity on Candida tropicalis (MIC 0.094 mg/mL). Staphylococcus aureus and Salmonella typhimurium growths were also inhibited by the oil at a concentration of 0.375 mg/mL. The prepared infusion of the flowering aerial parts showed weak antimicrobial effects against all the tested microorganisms, but demonstrated a remarkably radical scavenging activity (IC 50 : 3.9 µg/mL).

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Essential Oil Composition, Antimicrobial and Cytotoxic Activities of Two Endemic Stachys Cretica Subspecies (Lamiaceae) from Turkey

Cited by 12 publications

References 31 publications

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

Cytotoxic effect of methanolic extract, alkaloid and terpenoid fractions of Stachys pilifera against HT-29 cell line

Chemical Composition and In vitro Cytotoxic Activity of the Essential Oils of Stachys rupestris and Salvia heldreichiana, Two Endemic Plants of Turkey

Antimicrobial and Antioxidant Activities of Stachys lavandulifolia subsp. lavandulifolia Essential Oil and its Infusion

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