Essential Oil of Flowers from Eugenia klotzschiana (Myrtaceae): Chemical Composition and In Vitro Trypanocidal and Cytotoxic Activities

Abstract

Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated by nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine the chemical composition of the essential oil from fresh flowers of Eugenia klotzchiana and to evaluate for the first time its trypanocidal and cytotoxic potential in vitro. The essential oil from the fresh flowers was obtained by hydrodistillation carried out by a Clevenger apparatus and its chemical composition was determined by GC-MS. The main compounds found in the essential oil were ses uite pe oids: β-caryophyllene (21.1%), bicyclogermacrene (10.2%) and spathulenol (20.9%). Results showed that the essential oil had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC 50 =. μg/ L a d ode ate toto i it agai st fi o lasts i the o e t atio a ge CC 50 =. μg/ L. I sho t, the essential oil from fresh flowers of Eugenia klotzchiana can be considered a new source of bioactive compounds for the development of antiparasitic drugs.

Chemical Composition and In Vitro Antimicrobial Activity of the Essential Oil Obtained from Eugenia pyriformis Cambess. (Myrtaceae)

“…Previous studies have shown that β-caryophyllene and bicyclogermacrene are present in the essential oil obtained from species other than E. pyriformis. β-Caryophyllene is a major component of essential oil of E. klotzschiana [18], E. moraviana [19], E. argentea [5] and E. involucrata [20]; it is abundantly present in the essential oil of E. stipitata [21] and E. umbeliflora [19].…”

“…β-Caryophyllene and bicyclogermacrene are present in moderate amounts in the essential oil obtained from E. burkartiana [19], E. ramboi [19], E. repanda [22], E. cuprea, E. pitanga [23], E. beaurepaireana [23], E. hyemalis [23], E. mattosii [24] and E. klotzschiana [18,25].…”

“…Sesquiterpene compounds are widely found in the essential oils of medicinal plants and fruits and are used for the treatment of various conditions [26]. β-Caryophyllene has several properties such as anti-edema, phagorepullic, anti-inflammatory, antitumor, insecticidal, spasmolytic, bactericidal properties [18], and bicyclogermacrene has antifungal activity [27].…”

Analysis of the chemical profile of cerrado pear fixed compounds by mass spectrometry with paper spray and volatile ionization by SPME-HS CG-MS

“…It is a fruit consumed by the regional population in the form of jams, sweets, jellies, and natural juices. In the past, its consumption was more frequent, however, currently, there is a shortage of its trees, as a result of the deforestation that has occurred in the cerrado regions for raising beef cattle or wood plantations, reducing access and consumption (Carneiro, Alves, Alves, Esperandim, & Miranda, 2017).…”

Chemical composition, in vitro larvicidal and antileishmanial activities of the essential oil from Citrus reticulata Blanco fruit peel

“…Filtration was then carried out. EO was stored in an amber bottle and kept in a refrigerator at 4 °C until analysis (Carneiro et al, 2017).…”