Essential oil of Ocimum gratissimum L.: Anesthetic effects, mechanism of action and tolerance in silver catfish, Rhamdia quelen

Silva, Lenise de Lima; Parodi, Thaylise Vey; Reckziegel, Patrícia; Garcia, Viviane de Oliveira; Bürger, Marilise Escobar; Baldisserotto, Bernardo; Malmann, Carlos Augusto; Pereira, Ana Maria Soares; Heinzmann, Berta Maria

doi:10.1016/j.aquaculture.2012.04.012

Cited by 108 publications

(87 citation statements)

References 33 publications

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“…Higher induction times were observed for both EOO concentrations when compared to the previous report (Silva et al, 2012). The different induction times to anesthesia observed in both studies may have been influenced by biological factors such as age, sex, life stage, body weight, and physiological condition, as well as environmental factors such as water temperature, pH, and dissolved oxygen level (Zahl et al, 2012).…”

Section: Discussioncontrasting

confidence: 40%

“…It was stored at -4 ºC in amber glass bottles until the experiments were initiated. The major compounds of EOO were eugenol (76.3 %) and β-bisabolene (18.3 %) (Silva et al, 2012).…”

Section: Essential Oil Extractionmentioning

confidence: 99%

“…It has been used as a condiment and, in traditional medicine, as a sedative and in the treatment of stress, headache, among others (Albuquerque & Andrade, 1998;Di Stasi et al, 2002;Albuquerque et al, 2007). Recently, the essential oil of O. gratissimum (EOO) was also proposed as a new anesthetic for silver catfish juveniles (Silva et al, 2012;Benovit et al, 2012). Thus, the purpose of the present study was to evaluate the effects of such oil on the stress response induced by the handling of silver catfish.…”

Section: Introductionmentioning

confidence: 99%

“…Juveniles stayed in the aquarium until reaching stage 4 of anesthesia induction (Schoettger & Julin, 1967) with EOO or for 10 minutes in the case of the controls. This time was chosen for the controls because it corresponds to the highest induction time observed for EOO concentrations used in this study (Silva et al, 2012). Anesthesia was determined by loss of reflex activity and lack of reaction to strong external stimuli.…”

Section: General Proceduresmentioning

confidence: 99%

“…These EOO concentrations were chosen because they induced the anesthesia of silver catfish within 1 -6 minutes (Silva et al, 2012). Before dissolution in water, the maioria dos tratamentos, e estes eventos não diferiram entre os tratamentos.…”

Section: General Proceduresmentioning

confidence: 99%

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Effects of anesthesia with the essential oil of Ocimum gratissimum L. in parameters of fish stress

Silva

Garlet

Koakoski

et al. 2015

Rev. bras. plantas med.

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ABSTRACT:The effects of anesthesia with the essential oil of Ocimum gratissimum (EOO) in parameters of stress after handling were investigated in silver catfish (Rhamdia quelen). EOO was obtained from the aerial parts by hydrodistillation. Juveniles were anesthetized with 70 or 300 mg L -1 EOO and submitted to air exposure for 1 minute. The fishes were sampled immediately or transferred to anesthetic-free aquaria until sampling. In the first experiment, juveniles had their blood collected at 0, 1, 4, and 8 h after handling to assay plasma cortisol and blood glucose levels. The unanesthetized animals were restrained manually for blood collection. In the second experiment, water samples of the recovery aquaria were collected to evaluate net ion fluxes at 0 -4 h and 4 -8 h. Water and ethanol controls were also performed under the same conditions. The results showed that the cortisol levels did not differ among the treatments. Hyperglycemia was verified in fish exposed to 70 and 300 mg L -1 EOO at 1 h and 4 h after handling. After 8 h, cortisol and glucose concentrations were lower or similar than those from immediately after handling for all treatments. EOO anesthesia prevented Na + efflux observed in the control groups in both flux periods. There were net Cl -and K + effluxes at 0 -4 h and influxes at 4 -8 h after handling in most treatments, and these fluxes did not differ among the treatments. The results suggest that EOO did not impair stress recovery and did not act as an additional handling stressor in silver catfish.Key words: clove basil, cortisol, glucose, net ion flux, silver catfish. RESUMO: Efeitos da anestesia com o óleo essencial de Ocimum gratissimum L. em parâmetros de estresse de peixes. Os efeitos da anestesia com o óleo essencial deOcimum gratissimum (EOO) em parâmetros de estresse após manuseio foram investigados em jundiás (Rhamdia quelen). EOO foi obtido a partir das partes aéreas por hidrodestilação. Os juvenis foram anestesiados com 70 ou 300 mg L -1 de EOO e expostos ao ar por 1 minuto. Os peixes foram amostrados imediatamente ou transferidos para aquários sem anestésico até amostragem. No primeiro experimento, os juvenis tiveram seu sangue coletado em 0, 1, 4, e 8 h após manuseio para avaliar os níveis de cortisol e glicemia. Os animais não anestesiados foram contidos manualmente para coleta sanguínea. No segundo experimento, amostras de água foram coletadas do aquário de recuperação dos animais para avaliação do fluxo iônico entre 0 -4 h e 4 -8 h. Grupos controles em água e etanol também foram realizados sobre as mesmas condições. Os resultados demonstraram que os níveis de cortisol não diferiram entre os tratamentos. Hiperglicemia foi detectada em peixes expostos a 70 e 300 mg L -1 de EOO em 1 h e 4 h após o manuseio. Após 8 h, os teores de cortisol e glicose foram menores ou similares aqueles imediatamente após o manuseio para todos os tratamentos. A anestesia com EOO preveniu o efluxo de Na + observado para os grupos controle em ambos os períodos avaliados. Ocorreram efluxos de Cl-and ...

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Section: Discussioncontrasting

confidence: 40%

“…It was stored at -4 ºC in amber glass bottles until the experiments were initiated. The major compounds of EOO were eugenol (76.3 %) and β-bisabolene (18.3 %) (Silva et al, 2012).…”

Section: Essential Oil Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: General Proceduresmentioning

confidence: 99%

Section: General Proceduresmentioning

confidence: 99%

See 3 more Smart Citations

Effects of anesthesia with the essential oil of Ocimum gratissimum L. in parameters of fish stress

Silva

Garlet

Koakoski

et al. 2015

Rev. bras. plantas med.

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Anesthetic Efficiency of Three Medicinal Plant Oils for Aquatic Species: Coriander Coriandrum sativum, Linaloe Tree Bursera delpechiana, and Lavender Lavandula hybrida

et al. 2019

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This study evaluated the potential of three essential oils (EOs) that were obtained from coriander Coriandrum sativum (CEO), linaloe tree Bursera delpechiana (BEO), and lavender Lavandula hybrida (LEO) as anesthetic agents. Convict Cichlids Amatitlania nigrofasciata (Günther 1867) were exposed to eight concentrations of anesthetic (50, 75, 100, 125, 150, 200, 250, and 300 μL/L). After exposure to the anesthetic, the fish were transferred to clean water to recover. All of the EOs produced an anesthetic effect after exposure to the compounds for 30 min at the minimal effective concentration (MEC), which was identified according to deep anesthesia (A 5 < 3 min) and full recovery (R 3 < 5 min) times. At 50 and 75 μL/L, the total loss of equilibrium was not observed for all tested EOs. The total loss of reflex was induced at a faster rate with higher concentrations of anesthetic in all groups. The recovery time generally increased as the concentration of the anesthetic increased. These findings suggest that CEO, BEO, and LEO are all novel potential anesthetics for aquaculture, and the optimal concentrations were identified as 150 μL/L (A 5 ; 156 ± 1.7 s and R 3 ; 165 ± 2.9 s), 125 μL/L (A 5 ; 176 ± 3.5 s; R 3 ; 125 ± 2.0 s), and 200 μL/L (A 5 ; 20.1 ± 2.4 s and R 3 ; 162 ± 3.4 s), respectively. When considering the active ingredients of EOs, this study also demonstrated that future studies should be focused on the major components such as linalyl acetate, 1.8-cineole, α-pinene, geraniol, and linalool. Their synergistic effects should be examined in herbal anesthetic treatments, since new commercial anesthetics will likely contain them.

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Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens

2016

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Essential oil of Ocimum gratissimum L.: Anesthetic effects, mechanism of action and tolerance in silver catfish, Rhamdia quelen

Cited by 108 publications

References 33 publications

Effects of anesthesia with the essential oil of Ocimum gratissimum L. in parameters of fish stress

Effects of anesthesia with the essential oil of Ocimum gratissimum L. in parameters of fish stress

Anesthetic Efficiency of Three Medicinal Plant Oils for Aquatic Species: Coriander Coriandrum sativum, Linaloe Tree Bursera delpechiana, and Lavender Lavandula hybrida

Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens

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