Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens

Pattanasiri, Thanapat; Taparhudee, Wara; Suppakul, Panuwat

doi:10.1007/s10499-016-0020-2

Cited by 19 publications

(11 citation statements)

References 28 publications

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“…Thus, a high level of variability of MNT, ranging from 0 to the maximum of 20 N, was found for all time points after injection, and variability was larger after injection than before, especially at time points 1 and 24 h post-injections. The insensitivity of calves at some locations, i.e., the complete absence of a withdrawal response even following the application of the maximum possible mechanical pressure, seems to confirm previous observations on the anaesthetic effect of (iso)eugenol (e.g., [9,11,13,14]). The anaesthetic effect/insensitivity was most pronounced one hour after injection, although it was also observed 24 h later across several locations and animals.…”

Section: Calvessupporting

confidence: 88%

“…Isoeugenol as a pure substance (98% cis-and trans eugenol) is derived from eugenol by a heating process [10]. Clove oil and its main component eugenol, as well as isoeugenol, have anaesthetic and analgesic properties; they can be used locally such as in (human) dental medicine or systemically as is common in fish medicine (for review: [9]; in fish: [11][12][13]; in rodents: [14,15]). Clove oil and (iso)eugenol also exhibit cytotoxic effects: for instance, eugenol was shown to cause apoptosis in cancer cells in vitro and in animal models after different forms of systemic application [16].…”

Section: Introductionmentioning

confidence: 99%

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Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding

Frahm

Giminiani

Stanitznig

et al. 2020

Animals

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In this preliminary study, we compared changes in mechanical nociceptive thresholds (MNT) of calves and goat kids injected with clove oil or isoeugenol under the horn bud as a potential, more welfare-friendly alternative to hot-iron disbudding. Twenty male calves and goat kids were randomly allocated to clove oil (n = 10 per species) or isoeugenol (n = 10 per species) injection under the horn buds. MNT was measured via a pressure algometer in calves and kids at several locations around the horn buds at several time points before and up to 24 h after injection. In kids, von Frey filaments were used additionally at the same time points. In calves, linear mixed models revealed an effect on MNT of time point (p = 0.010) and side (p = 0.007), but not of injection (p = 0.298), nor of the interaction ‘injection*time point’, MNT waslowest 9 h post-injection. In goats, there was an effect of injection depending on time point (interaction injection*time point, p = 0.03) with MNT being lowest 24 h post-injection for clove oil, while MNT was similar to pre-injection in isoeugenol. In both species, variation in the individual response post-injection was very high. Our results suggest that clove oil and isoeugenol induced hypersensitivity, which was higher for clove oil, in goat kids, but they also suggest a transient anaesthetic effect in some animals and locations.

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Section: Calvessupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding

Frahm

Giminiani

Stanitznig

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…Data are presented as mean ± standard deviation (n ¼ 12 anaesthetic for the following electronarcosis experiment, due to two main reasons: it presents reliably safety both for fish and for the operator in industry in the dose used and its sedation is lighter than that produced by the synthetic anaesthetics, which is often preferable in fish aquaculture (Pattanasiri et al 2017). Moreover, eugenol is more economically affordable when compared to benzocaine.…”

Section: Discussionmentioning

confidence: 99%

Effects of different stunning methods on blood markers and enzymatic activity of stress responses of tilapia (Oreochromis niloticus)

Baldi

Parisi

Costa

et al. 2018

Italian Journal of Animal Science

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“…Although eugenol use is not allowed in the United Kingdom, Canada, and the United States, its use as a fish anaesthetic has been extensively reported (Bolasina et al, ; Cooke et al, ; Pattanasiri, Taparhudee, & Suppakul, ). Eugenol has been used legally as a food fish anaesthetic in many countries to assist fish handling and transport, such as in Japan (Ministry of Agriculture, Forestry, & Fisheries of Japan, ), New Zealand (Ministry for Primary Industries of New Zealand, ), Australia (Australian Government, ), and the European Union (European Medicines Agency, ).…”

Section: Discussionmentioning

confidence: 99%

The efficacy of eugenol and tricaine methanesulphonate as anaesthetics for juvenile Chinese sea bass (Lateolabrax maculatus) during simulated transport

et al. 2018

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Summary The purpose of this study was to evaluate the anaesthesia effects of eugenol and MS‐222 sedatives applied on juvenile Lateolabrax maculatus during simulated transport. In experiment 1, the juveniles were divided into two groups, with seven concentrations tested on each group (eugenol [4, 6, 8, 10, 12, 14 and 16 mg/L] and MS‐222 [20, 30, 40, 50, 60, 70 and 80 mg/L]). Induction and recovery times were recorded. The time for anaesthesia was shortened, and the time for complete recovery was prolonged as the anaesthetic concentration increased. The optimal transport concentration for each anaesthetic tested was 6 mg/L of eugenol and 30 mg/L for MS‐222. In experiment 2, the 5‐hr simulated transport test showed that the survival rate of L. maculatus juveniles with anaesthesia was 100%, and without anaesthesia, survival was 60%. After 24 hr of recovery following transport, the fish showed 100% survival for the group with added anaesthetic and 40% for the group without added anaesthetic. Compared to the non‐anaesthetized groups, the anaesthetized transport groups showed significant increases in the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (p < 0.05). The levels of AST, ALT and alkaline phosphatase (AKP) were significantly higher in the MS‐222 transport group than in the eugenol transport group (p < 0.05). The levels of AKP were significantly higher in the non‐anaesthetized transport group than in the anaesthetized group (p < 0.05). According to the present experiment results, eugenol was an efficient anaesthetic in L. maculatus, and we recommend eugenol instead of MS‐222 as an anaesthetic for the short‐time transport of L. maculatus.

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Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens

Cited by 19 publications

References 28 publications

Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding

Nociceptive Threshold of Calves and Goat Kids Undergoing Injection of Clove Oil or Isoeugenol for Disbudding

Effects of different stunning methods on blood markers and enzymatic activity of stress responses of tilapia (Oreochromis niloticus)

The efficacy of eugenol and tricaine methanesulphonate as anaesthetics for juvenile Chinese sea bass (Lateolabrax maculatus) during simulated transport

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