Essential oil of Syringa oblata Lindl. as a potential biocontrol agent against tobacco brown spot caused by Alternaria alternata

Jing, Changliang; Zhao, Jianlong; Han, Xiaobin; Huang, Rui-Huan; Cai, Desheng; Zhang, Chengsheng

doi:10.1016/j.cropro.2017.10.002

Cited by 39 publications

(26 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In subsequent experiments, the fluorescent microscopy observation also confirmed that carvacrol and thymol destroyed mycelial membranes of B. cinerea in this study. Previous studies have shown that EO could lead to the rupture and damage of fungal cell membrane (Yu et al, 2015) and abnormal transport function, nucleic acid and intracellular protein leakage, and abnormal metabolism, which is the possible mechanism of antifungal action of EOs (Chen et al, 2014;Jing et al, 2018). The relative leakage test showed that 200 mg/mL carvacrol and thymol could significantly increase the relative permeability of cells and 10 mg/L carvacrol and 70 mg/L thymol at the treatment time points of (0-400 min) were equivalent to that of vinclozolin.…”

Section: Discussionmentioning

confidence: 99%

“…B. cinerea isolates were cultured on PDA plates at 20 °C for 14 d. Then, the plates were injected with five ml sterile water and gently scraped with a sterile swab and filtered through four layers of sterile cheesecloth to obtain spore suspension ( Jing et al, 2018 ). The spore suspension concentration was adjusted to 10 5 ~10 6 spores mL −1 by using a hemocytometer and continuously adding sterile water.…”

Section: Methodsmentioning

confidence: 99%

“…Antifungal activity of OVEO, carvacrol and thymol on spore germination of B. cinerea B. cinerea isolates were cultured on PDA plates at 20 C for 14 d. Then, the plates were injected with five ml sterile water and gently scraped with a sterile swab and filtered through four layers of sterile cheesecloth to obtain spore suspension (Jing et al, 2018).…”

Section: Gc-ms Analysismentioning

confidence: 99%

See 2 more Smart Citations

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Hou

Zhang

Zhao

et al. 2020

PeerJ

View full text Add to dashboard Cite

Background Botrytis cinerea causes serious gray mold disease in many plants. This pathogen has developed resistance to many fungicides. Thus, it has become necessary to look for new safe yet effective compounds against B. cinerea. Methods Essential oils (EOs) from 17 plant species were assayed against B. cinerea, of which Origanum vulgare essential oil (OVEO) showed strong antifungal activity, and accordingly its main components were detected by GC/MS. Further study was conducted on the effects of OVEO, carvacrol and thymol in vitro on mycelium growth and spore germination, mycelium morphology, leakages of cytoplasmic contents, mitochondrial injury and accumulation of reactive oxygen species (ROS) of B. cinerea. The control efficacies of OVEO, carvacrol and thymol on tomato gray mold were evaluated in vivo. Results Of all the 17 plant EOs tested, Cinnamomum cassia, Litsea cubeba var. formosana and O. vulgare EOs had the best inhibitory effect on B. cinerea, with 0.5 mg/mL completely inhibiting the mycelium growth of B. cinerea. Twenty-one different compounds of OVEO were identified by gas chromatography–mass spectrometry, and the main chemical components were carvacrol (89.98%), β-caryophyllene (3.34%), thymol (2.39%), α-humulene (1.38%) and 1-methyl-2-propan-2-ylbenzene isopropyl benzene (1.36%). In vitro experiment showed EC50 values of OVEO, carvacrol and thymol were 140.04, 9.09 and 21.32 μg/mL, respectively. Carvacrol and thymol completely inhibited the spore germination of B. cinerea at the concentration of 300 μg/mL while the inhibition rate of OVEO was 80.03%. EC50 of carvacrol and thymol have significantly (P < 0.05) reduced the fresh and dry weight of mycelia. The collapse and damage on B. cinerea mycelia treated with 40 μg/mL of carvacrol and thymol was examined by scanning electron microscope (SEM). Through extracellular conductivity test and fluorescence microscope observation, it was found that carvacrol and thymol led to increase the permeability of target cells, the destruction of mitochondrial membrane and ROS accumulation. In vivo conditions, 1000 μg/mL carvacrol had the best protective and therapeutic effects on tomato gray mold (77.98% and 28.04%, respectively), and the protective effect was significantly higher than that of 400 μg/mL pyrimethanil (43.15%). While the therapeutic and protective effects of 1,000 μg/mL OVEO and thymol were comparable to chemical control. Conclusions OVEO showed moderate antifungal activity, whereas its main components carvacrol and thymol have great application potential as natural fungicides or lead compounds for commercial fungicides in preventing and controlling plant diseases caused by B. cinerea.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Gc-ms Analysismentioning

confidence: 99%

See 1 more Smart Citation

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Hou

Zhang

Zhao

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…All EOs and fungicides significantly p ≤ 0.05 decreased the Table 6 . Application of EOs against soil born pathogen 15,43 , foliar fungal disease 8,38 , and postharvest disease 7,10,11,20 was reported by other researchers. The high rate of phenolic compounds including thymol and carvacrol in plant essential oils prevents the growth of a fungal colony of Alternaria citri causal agent of orange black rot and reduces the severity of decay 7 .…”

Section: Scanning Electron Microscopy Analysismentioning

confidence: 81%

Management of Strawberry Anthracnose Using Plant Essential Oils as Bio-fungicides, and Evaluation of Their Effects on Quality of Strawberry Fruit

et al. 2020

View full text Add to dashboard Cite

Introduction Kurdistan Provence west of Iran is the most important habitat of strawberry Fragaria ananassa Duch. in Iran. Strawberry anthracnose disease, caused by Colletotrichum nymphaeae, is one of the main pre-harvest and post-harvest diseases that affect strawberry production in Iran. Several species of Colletotrichum such as C. acutatum J.H Simmonds, C. fragariae Brooks, C. gloeosporioides Penz , and C. nymphaeae Pass. cause strawberry anthracnose disease 1. C. nymphaeae is one of the important pathogens causing strawberry anthracnose diseases in Kurdistan province, Iran. This widespread, aggressive species 2 is a limiting factor in strawberry production. The symptoms appear on the aerial parts of the strawberry plant i.e., stolon, petiole, flower, fruit, leaf, and crown , especially fruit decay such as sunken lesions and conidial ooze on fruits 1. Resistance cultivar to this fungus has not been found and reported in Iran yet. Application of chemical fungicides on fruit against the disease is limited and

show abstract

“…Each plot was sampled at 5 points, the disease index of 10 tobacco plants were recorded at each point, and the total number of investigated tobacco plants and the number of diseased tobacco plants at each grade was recorded. The disease index was computed and shown below as previously described (10, 35, 48). …”

Section: Methodsmentioning

confidence: 99%

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Guo

Yuan

Luo

et al. 2019

Preprint

View full text Add to dashboard Cite

Abstract： 9Tobacco black shank (TBS) caused by Phytophthora nicotianae is destructive to almost all 10 kinds of tobacco cultivars and is widespread in many tobacco-planted countries. Here, an isolated 11 plant growth-promoting rhizobacteria (PGPR) strain Ba168 is promise in biocontrol of TBS. In 12 vitro assays disclosed a strong P. nicotianae suppression activity and the field utilization potential 13 (FUP) by characterized the crude extract of culture filtrates of Ba168. P. nicotianae's growth was 14 inhibited by the crude extract at minimum inhibitory concentration (MIC) of 5μl/mL. Extracellular 15 conductivity, pH and the wet, dry weight of P. nicotianae's mycelia, were significantly different 16 after treated with different concentrations of the crude extract and the deformity and perforation of 17 treated P. nicotianae's hyphae can be observed in scanning electron microscope (SEM) analysis. 18Proteome characterizations of the crude extract were used as supplementary proofs that further 19 evaluated FUP of Ba168. We then identified strain Ba168 as B. amyloliquefaciens by its genetic 20 and phenotypic characteristics. Field assays comparatively evaluated TBS control efficacy of these 21PGPRs and agrochemicals. Pooling analysis of the results showed that the biocontrol efficacy of 22Ba168 preparation is only lower than Mixture of Propamocarb hydrochloride and Azoxystrobin 23 (MPA) but better than other tested subjects. Although the existence of differences in biocontrol 24 efficacy, PGPR preparations effectively reduced the disease index of tobacco. 25

show abstract

Essential oil of Syringa oblata Lindl. as a potential biocontrol agent against tobacco brown spot caused by Alternaria alternata

Cited by 39 publications

References 24 publications

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Effects of Origanum vulgare essential oil and its two main components, carvacrol and thymol, on the plant pathogen Botrytis cinerea

Management of Strawberry Anthracnose Using Plant Essential Oils as Bio-fungicides, and Evaluation of Their Effects on Quality of Strawberry Fruit

Biocontrol of PGPR strain Bacillus amyloliquefaciens Ba168 against Phytophthora nicotianae on tobacco

Contact Info

Product

Resources

About