Essential Role of the Chaperonin CCT in Rod Outer Segment Biogenesis

Sinha, Satyabrata; Belcastro, Marycharmain; Datta, Poppy; Seo, Seongjin; Sokolov, Maxim

doi:10.1167/iovs.14-13889

Cited by 13 publications

(13 citation statements)

References 40 publications

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“…To date, the only data reported on the amphibian homologue of TCP1 concern the expression of tcp1a (CCTa) in the axolotl (Ambystoma mexicanum), in which it is spatially and temporally regulated during neural and somitic development (Sun et al, 1995), and the expression in Xenopus laevis embryos of tcp1a and tcp1g, which appear to be developmentally regulated in neural-derived and myogenic lineages (Dunn and Mercola, 1996). Recently, we selected pfdn6a and tcp1a in the course of a screen aimed at identifying genes coherently controlled by the retinal key regulator Rx1 (Giannaccini et al, 2013;Giudetti et al, 2014), consistently with previous data indicating a role of PFDN and TCP1 in retinal development (Matsuda and Mishina 2004;Lee et al, 2011Sinha et al, 2014. The coregulation of these two genes by Rx1 suggested that they could display a general coordinated expression during embryogenesis.…”

supporting

confidence: 82%

“…In mouse, tcp1a is observed at the morula and blastocyst stages and its expression is higher in post implantation embryos than in any adult organs except testis, indicating that tcp1a expression is regulated spatially and temporally during embryogenesis (Kubota et al, 1992). Finally, suppression of TCP1 activity in mouse photoreceptors leads to malformation of the outer segments and triggers retinal degeneration, indicating an essential role of TCP1 in the differentiation and survival of this photosensitive cell type (Sinha et al, 2014).…”

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confidence: 99%

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Comparative expression analysis of pfdn6a and tcp1? during Xenopus development

Marracci¹,

Martini²,

Giannaccini³

et al. 2015

Int. J. Dev. Biol.

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We recently identified pfdn6a and tcp1a (also known as cct-a) as genes coregulated by the transcription factor Rx1. The proteins encoded by these genes belong to two interacting complexes (Prefoldin and "chaperonin containing t-complex polypeptide 1"), which promote the folding of actin and tubulin and have more recently been reported to be involved in a variety of additional functions including cell cycle control and transcription regulation. However, little is known about the expression and function of these two genes during vertebrate development. To assess whether pfdn6a and tcp1a display a general coordinated expression during Xenopus development, we determined, by RT-PCR and in situ hybridization, the spatio-temporal expression pattern of pfnd6a, which was not previously described, and compared it to that of tcp1a, extending the analysis to stages not previously investigated for this gene. We detected maternal transcripts of pfnd6a in the animal hemisphere at early blastula stage. During gastrulation, pfdn6a was expressed in the involuting mesoderm and subsequently in the anterior and dorsal neural plate. At tailbud and tadpole stages, pfdn6a RNA was mainly detected in the forebrain, midbrain, eye vesicle, otic vesicle, branchial arches, and developing pronephros. The pfnd6a expression pattern largely overlaps with that of tcp1a indicating a spatio-temporal transcriptional coregulation of these genes in the majority of their expression sites, which is suggestive of a possible involvement in the same developmental events.

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supporting

confidence: 82%

mentioning

confidence: 99%

Comparative expression analysis of pfdn6a and tcp1? during Xenopus development

Marracci¹,

Martini²,

Giannaccini³

et al. 2015

Int. J. Dev. Biol.

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“…Chaperone T-complex protein 1 has been extensively studied in humans and animals (Sinha et al, 2014; Wu et al, 2015), but the function of chaperone T-complex protein 1 remains poorly understood in plants. In this study, drought significantly elevated the expression of T-complex protein 1 subunits zeta (B6U118) and delta (C0HGT5) in an ABA-dependent way.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Proteomic Analyses Identify ABA-Related Proteins and Signal Pathways in Maize Leaves under Drought Conditions

et al. 2016

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Drought stress is one of major factors resulting in maize yield loss. The roles of abscisic acid (ABA) have been widely studied in crops in response to drought stress. However, more attention is needed to identify key ABA-related proteins and also gain deeper molecular insights about drought stress in maize. Based on this need, the physiology and proteomics of the ABA-deficient maize mutant vp5 and its wild-type Vp5 under drought stress were examined and analyzed. Malondialdehyde content increased and quantum efficiency of photosystem II decreased under drought stress in both genotypes. However, the magnitude of the increase or decrease was significantly higher in vp5 than in Vp5. A total of 7051 proteins with overlapping expression patterns among three replicates in the two genotypes were identified by Multiplex run iTRAQ-based quantitative proteomic and liquid chromatography-tandem mass spectrometry methods, of which the expression of only 150 proteins (130 in Vp5, 27 in vp5) showed changes of at least 1.5-fold under drought stress. Among the 150 proteins, 67 and 60 proteins were up-regulated and down-regulated by drought stress in an ABA-dependent way, respectively. ABA was found to play active roles in regulating signaling pathways related to photosynthesis, oxidative phosphorylation (mainly related to ATP synthesis), and glutathione metabolism (involved in antioxidative reaction) in the maize response to drought stress. Our results provide an extensive dataset of ABA-dependent, drought-regulated proteins in maize plants, which may help to elucidate the underlying mechanisms of ABA-enhanced tolerance to drought stress in maize.

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“…One group of the HSP70 family, CCT (Chaperonin containing Tcp1), was shown to be essential for elongation and extension of the rod outer segments, i.e. mice deficient in CCT were unable to build outer segments due to the impairment of ciliary trafficking (Posokhova et al, 2011; Sinha et al, 2014). It has been reported that tubb y mice exhibited blockage of transport of selective G protein-coupled receptors (GPCRs) to the neuronal primary cilia and aberrant rhodopsin trafficking, indicating that the Tubby protein is a required part of the transport machinery in retinal cells (Sun et al, 2012).…”

Section: Discussionmentioning

confidence: 99%