Estabelecimento in vitro e crescimento inicial de Physallis angulata (Solanaceae)

Abstract

Resumo -O objetivo deste estudo foi estabelecer Physalis angulata in vitro e identificar o meio de cultura mais adequado para a germinação e crescimento inicial da espécie. Nós avaliamos a germinação, o estabelecimento in vitro e o crescimento inicial de suas plântulas em diferentes meios de cultura (MS, WPM e ½MS). Primeiro, foram avaliados porcentagem, tempo médio e índice de velocidade de germinação 15 dias após a inoculação das sementes. Em seguida, foram avaliados número médio de raízes e folhas, comprimento da parte aérea e da raiz, e massa seca da parte aérea, foliar e da raiz de plântulas após 15, 30 e 45 dias da inoculação das sementes. A germinação iniciou-se quatro dias após a inoculação e não foi afetada pelos meios de cultura. Por outro lado, foram observadas diferenças significativas entre os tipos de meio de cultura para a maioria dos parâmetros de crescimento avaliados. Os meios de cultura WPM e ½MS foram mais adequados para o desenvolvimento inicial das plântulas, sendo recomendados para as demais etapas da micropropagação da espécie. Palavras-chave adicionais: Camapu, micropropagação, meio de cultura, planta medicinal.Abstract (In vitro establishment and initial growth of Physalis angulata (Solanaceae)) -The aim of this study was to establish Physalis angulata in vitro and identify the best culture medium for germination and initial growth of the species. We assessed the germination, in vitro establishment and initial growth of its seedlings in different culture media (MS, WPM and ½MS). First, we evaluated the percentage, average time and speed index of germination 15 days after seed inoculation. Then, we evaluated the average number of roots and leaves, length of shoots and roots, and dry mass of shoots, leaves and roots of seedlings 15, 30 and 45 days after seed inoculation. Germination started four days after inoculation and the choice of culture media did not affect germination. However, significant differences among culture media were found for most growth parameters evaluated. The culture media WPM and ½MS provide better initial growth of seedlings and these are recommended for the other stages of micropropagation of this species.

Ecophysiological aspects of the germination of Physalis angulata L. seeds

“…The results of the germination speed index (GSI), under the absence of light, were not only higher than those obtained under the other light conditions, but was also higher than those obtained in other studies with P. angulata under white light (LANNA et al, 2013;OLIVEIRA et al, 2013). On the other hand, the GSI values obtained by Souza, Souza, and Pelacini (2011) and Oliveira et al (2013) are consistent with those observed in this study under white light.…”

“…On the other hand, light at far-red frequency (FR) caused lower results for most of the variables analyzed. With seeds of P. angulata, in the presence of light, Carvalho et al (2014) and Oliveira et al (2013) obtained germination above 80%. However, the results of this study do not allow the seeds of this species to be classified as positive photoblastic.…”

“…As for temperature, for most plant species of tropical climate, the optimal value for seed germination occurs in the interval between 15 and 30 ºC (TAIZ et al, 2015). For some species of the genus Physalis, such as P. ixocarpa and P. peruviana, or even for P. angulata sown in plant culture medium (in vitro), the constant temperature of 25 ºC promoted germination above 80% (LANNA et al, 2013;OLIVEIRA et al, 2013).…”

“…-dez., 2023 ECOPHYSIOLOGICAL ASPECTS OF THE GERMINATION OF Physalis angulata L. SEEDS W. R. SANTIAGO et al Most of the research with germination of P. angulata was conducted under a 12-h photoperiod, obtaining percentages above 80% (SOUZA et al, 2010;PELACINI, 2011;SOUZA et al, 2014). However, Oliveira et al (2013) obtained, in vitro, germination above 80%, under the 16-h photoperiod, at 25 ºC, while Carvalho et al (2014) obtained germination of 89% with 8-h photoperiod, under the alternating temperature of 20-30 ºC. For P. peruviana, Diniz, Chamma, and Novembre (2020) consider that the 8-h photoperiod is a more adequate condition than the absence of light during the germination test.…”

“…Caatinga, Mossoró, v. 36, n. 4, p. 980 -987, out. -dez., 2023 ECOPHYSIOLOGICAL ASPECTS OF THE GERMINATION OF Physalis angulata L. SEEDS W. R. SANTIAGO et al commonly used (LANNA et al, 2013;OLIVEIRA et al, 2013;SOUZA et al, 2014;GAIER et al, 2019). Temperature is one of the main abiotic factors that influence germination.…”