Establishing a campylobacter-free pig population through a top-down approach

Weijtens, M.J.B.M.; Urlings, H.A.P.; Plas, J. van der

doi:10.1046/j.1472-765x.2000.00750.x

Cited by 21 publications

(18 citation statements)

References 11 publications

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“…As reported for Campylobacter coli in pigs (38,49,50), some Arcobacter strains spread over the pig house, resulting in dominant strains in several animals. For example strains B1, B4, B8, and B11 were predominantly excreted by several pigs throughout the finishing period on farm D. The dominance of such strains may suggest a greater capacity to survive the environmental stresses and to colonize the host gut, as previously described for Campylobacter jejuni (12,33).…”

Section: Discussionmentioning

confidence: 67%

Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

Smet

Zutter

Debruyne

et al. 2011

Appl Environ Microbiol

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Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 11.3 to 50.0%, with excretion levels of up to 10 4 CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur.

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Section: Discussionmentioning

confidence: 67%

Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

Smet

Zutter

Debruyne

et al. 2011

Appl Environ Microbiol

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“…Weijtens et al (2000) reported that 22-98% of fecal samples from fattening pigs in the Netherlands were positive for Campylobacter. A similar study from the USA reported that 70-100% of pigs at slaughter (depending on the farm and the date samples were collected) were positive for Campylobacter (Harvey et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of thermophilic Campylobacter spp. in slaughtered pigs in the Czech Republic, 2001-2003

Steinhauserova¹,

Nebola²,

Mikulicova³

2005

Vet. Med. - Czech

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“…The apparent high exposure from the paddock environment implies that Campylobacter spp. infections in organic pigs might be more persistent, not least due to the difficulties in cleaning of the outdoor paddocks and practicing good hygiene, which is important to reduce infections Weijtens et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The occurrence and characterization of Campylobacter jejuni and C. coli in organic pigs and their outdoor environment

Jensen¹,

Dalsgaard²,

Baggesen³

et al. 2006

Veterinary Microbiology

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The occurrence and species distribution of thermophilic Campylobacter was investigated in organic outdoor pigs. An increased exposure of outdoor pigs to C. jejuni from the environment may cause a shift from a normal dominance of C. coli to more C. jejuni, which may imply a concern of reduced food safety.Bacteriological methods for determination of Campylobacter excretion level were combined with colony-blot hybridization and real-time PCR for specific detection of C. jejuni in pigs. Campylobacter was isolated from pigs (n = 47), paddock environment (n = 126) and wildlife (n = 44), identified to species by real-time PCR and sub-typed by serotyping (Penner) and pulse-field gel electrophorsis (PFGE) genotyping.All pigs excreted Campylobacter (10 3 -10 7 CFU g À1 faeces) from the age of 8-13-weeks old. C. jejuni was found in 29% of pigs in three consecutive trials and always in minority to C. coli (0.3-46%). C. jejuni and C. coli were isolated from 10% and 29% of the environmental samples, respectively, while crow-birds and rats harboured C. jejuni. Individual pigs hosted several strains (up to nine serotypes). The paddock environment was contaminated with C. coli serotypes similar to pig isolates, while most of the C. jejuni serotypes differed. C. jejuni isolates of different origin comprised few similar serotypes, just one identical genotype was common between pigs, environment and birds.In conclusion, the occurrence of C. jejuni varied considerably between the three groups of outdoor pigs. Furthermore, transfer of C. jejuni to the outdoor pigs from the nearby environment was not predominant according to the subtype dissimilarities of the obtained isolates. #

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Establishing a campylobacter-free pig population through a top-down approach

Cited by 21 publications

References 11 publications

Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

Prevalence of thermophilic Campylobacter spp. in slaughtered pigs in the Czech Republic, 2001-2003

The occurrence and characterization of Campylobacter jejuni and C. coli in organic pigs and their outdoor environment

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