Establishing a mouse model of choroidal neovascularization to study the therapeutic effect of levotinib and its mechanism

Abstract

Objective To study the therapeutic effect and mechanism of levotinib on choroidal neovascularization (CNV) in mice. Methods 45 healthy C57BL/6 mice were selected and randomly divided into three groups: control group (group A), model group (group B) and levotinib group (group C). The model of CNV in mice was established. The fluorescence leakage of choroidal lesions in mice was observed by fundus fluorescein angiography. The morphological changes of retinal vessels in mice were observed by retinal slice preparation, the pathological changes of eyeball tissues in mice were observed by hematoxylin-eosin (HE) staining, the expression of vascular endothelial growth factor (VEGF) in mice retina was detected by real-time quantitative fluorescence PCR, and the protein expression of VEGF in mice retina was detected by Western blotting. Result On the 7th, 14th and 21st day after modeling, compared with group B, the fluorescence leakage area of group C mice was significantly reduced, and the difference was statistically significant (P < 0.05). The morphology of retinal vessels in group A was normal. In group B, the retinal vessels showed large areas of ischemia without perfusion and abundant neovascularization clusters and capillaries. Compared with group B, the morphology of retinal vessels in group C was significantly improved. Group A mice had normal eyeball structure, group B mice had visible spindle-like damage to the inner and outer retina, while group C mice had significantly less spindle-like damage than group B. Compared with group A, group B mice had significantly higher expression of retinal VEGF and the difference was statistically significant (P < 0.05), but compared with group B mice, the expression of VEGF in the retina of mice in group C was significantly decreased, and the difference was statistically significant (P < 0.05). Compared with group A, the expression of VEGF in retina of group B mice was significantly increased, and the difference was statistically significant (P < 0.05). Compared with group B, the expression of VEGF in retina of group C mice was significantly decreased, and the difference was statistically significant (P < 0.05). Conclusion Levatinib has obvious therapeutic effect on CNV, which may be achieved by inhibiting the high expression of VEGF in CNV.

The Antiangiogenic Effect of Sanguinarine Chloride on Experimental Choroidal Neovacularization in Mice via Inhibiting Vascular Endothelial Growth Factor

“…Laser-induced CNV lesions were induced following a standard protocol ( Xin et al, 2020 ). In brief, animals were first anesthetized by intraperitoneal injection of avertin (2,2,2-tribromoethanol) (0.2 ml/10 g) (Nan Jing AIBI Bio-Technology Co., Ltd, Nanjing, China).…”

“…Hematoxylin and eosin (H&E) staining was performed according to a previously described procedure ( Wei et al, 2018 ; Xin et al, 2020 ). Mice were killed, and the eyes were enucleated 3°days (for safety test) or 7°days (for CNV volume analysis) after laser injury and fixed in 4% paraformaldehyde at 4 ° C for 24°h, conventionally dehydrated, and embedded in paraffin wax.…”

Prevention of Inflammation, Neovascularization, and Retinal Dysfunction by Kinin B1 Receptor Antagonism in a Mouse Model of Age-Related Macular Degeneration

“…Laser burns were given carefully to avoid large vessels, but as close as possible to the optic nerve head (around 500 µm, see Section 3). Laser-induced disruption of Bruch's membrane was identified by the appearance of a bubble at the site of laser lesion [19][20][21]. In 16% of the cases, the laser spot was, however, localized more peripherally due to the anatomy of the vascular bed.…”

Dietary astaxanthin-rich extract ameliorates atherosclerosis/retinopathy and restructures gut microbiome in apolipoprotein E-deficient mice fed on a high-fat diet

“…Before the test, mice were given an intraperitoneal injection of 5% chloral hydrate (10 mL per kg body weight) to produce hypnosis. 29,30 Then, 1% tropicamide eye drops were dropped into the eye for mydriasis, and levofloxacin transparent gel was used to protect the cornea.…”