Establishing and maintaining cell polarity with mRNA localization in <i>Drosophila</i>

Barr, Justinn; Yakovlev, Konstantin V.; Shidlovskii, Yulii V.; Schedl, Paul

doi:10.1002/bies.201500088

Cited by 14 publications

(12 citation statements)

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“…Promoter-driven loading of Paip2 could be a way to mark specific transcripts involved in RNA-depending tissue-specific events, e.g. cell polarization [43], neuroplasticity [44], etc. Another possibility, not excluding the aforementioned one, is the involvement of Paip2 in some event(s) at the promoter.…”

Section: Discussionmentioning

confidence: 99%

Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila

et al. 2018

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Paip2 (Poly(A)-binding protein - interacting protein 2) is a conserved metazoan-specific protein that has been implicated in regulating the translation and stability of mRNAs. However, we have found that Paip2 is not restricted to the cytoplasm but is also found in the nucleus in Drosophila embryos, salivary glands, testes, and tissue culture cells. Nuclear Paip2 is associated with chromatin, and in chromatin immunoprecipitation experiments it maps to the promoter regions of active genes. However, this chromatin association is indirect, as it is RNA-dependent. Thus, Paip2 is one more item in the growing list of translation factors that are recruited to mRNAs co-transcriptionally.

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Section: Discussionmentioning

confidence: 99%

Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila

et al. 2018

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“…Once the transcripts are on site, RNAbinding proteins interact with them to regulate their translation. The CPEB protein family is a group of translation factors that help anchor and control the on-site translation of localized transcripts [35][36][37][38][39][40].…”

Section: Discussionmentioning

confidence: 99%

“…Once the transcripts are on site, RNA-binding proteins interact with them to regulate their translation. The CPEB protein family is a group of translation factors that help anchor and control the on-site translation of localized transcripts [35–40]. CPEBs recognize CPE elements in the 3’UTR of localized transcripts and can function to repress or activate their translation, depending on the context.…”

Section: Discussionmentioning

confidence: 99%

The 3’UTR of theorb2gene encoding theDrosophilaCPEB translation factor plays a critical role in spermatogenesis

Gilmutdinov

Kozlov

Olenina

et al. 2020

Preprint

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CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these proteins in Drosophila , Orb2, is a principal player in spermatogenesis. It is required for meiosis and spermatid differentiation. During the later process orb2 mRNAs and proteins are localized within the developing spermatid. To evaluate the role of orb2 mRNA 3’UTR in spermatogenesis, we used the CRISPR/Cas9 system to generate a deletion of the orb2 3’UTR, orb2 R . This deletion disrupts the process of spermatid differentiation, but has no apparent effect on meiosis. While this deletion appears to destabilize the orb2 mRNA and reduce the levels of Orb2 protein, this is not the primary cause of the differentiation defects. Instead, differentiation appears to be disrupted because orb2 mRNAs and proteins are not properly localized within the differentiating spermatids. Other transcripts and proteins involved in spermatogenesis are also mislocalized in orb2 R spermatids.

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“…In neurons, the axons contain ribosomes and the translational machinery necessary for local translation of a specific subset of mRNAs [51]. While mRNA transport to specific localization of subcellular domains ultimately rely on a pre-existing cell polarity, on-site translation is efficient to generate and stabilize protein asymmetry [52]. With regard to polarity proteins, local translation of Pard3 in the growth cone has been observed in response to NGF and Netrin-1 signaling and to promote axonal elongation in neurons in culture [53].…”

Section: Post-transcriptional Regulation Of the Polarity Complexmentioning

confidence: 99%

Regulation of Polarity Protein Levels in the Developing Central Nervous System

Laumonnerie

Solecki

2018

Journal of Molecular Biology

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In the course of their development from neuroepithelial cells to mature neurons, neuronal progenitors proliferate, delaminate, differentiate, migrate, and extend processes to form a complex neuronal network. In addition to supporting the morphology of the neuroepithelium and radial glia, polarity proteins contribute to the remodeling of processes and support the architectural reorganizations that result in axon extension and dendrite formation. While a good amount of evidence highlights a rheostat-like regulation by signaling events leading to local activation and/or redistribution of polarity proteins, recent studies demonstrate a new paradigm involving a switch-like regulation directly controlling the availability of polarity protein at specific stage by transcriptional regulation and/or targeted ubiquitin proteasome degradation. During the process of differentiation, most neurons will adopt a morphology with reduced polarity which suggests that polarity complex proteins are strongly repressed during key step of development. Here we review the different mechanisms that directly impact the levels of polarity complex proteins in neurons in relation to the polarization context and discuss why this transient loss of polarity is essential to understand neural development and how this knowledge could be relevant for some neuropathy.

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Establishing and maintaining cell polarity with mRNA localization in Drosophila

Cited by 14 publications

References 100 publications

Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila

Paip2 is localized to active promoters and loaded onto nascent mRNA in Drosophila

The 3’UTR of theorb2gene encoding theDrosophilaCPEB translation factor plays a critical role in spermatogenesis

Regulation of Polarity Protein Levels in the Developing Central Nervous System

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