Establishing axenic cultures from mature pecan embryo explants on media with low water availability

Obeidy, Ahmed A.; Smith, M. Scott

doi:10.1007/bf00232274

Cited by 7 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of internal fungal contamination of mature pecan seeds was reported previously (Beuchat 1975;Chipley and Heaton 1971;Hanlin 1971;Schroeder and Cole 1977). Rigorous surface sterilization procedures could not avoid fungal contamination without disturbing explant viability (Obeidy and Smith 1990;. Therefore, a preliminary study was carried out to find an effective sterilization procedure required for culture maintenance.…”

mentioning

confidence: 84%

See 1 more Smart Citation

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

Renukdas

Manoharan

Garner

2010

Plant Biotechnology

View full text Add to dashboard Cite

The pecan [Carya illinoinensis (Wangenh.) K. Koch], a member of the family Juglandaceae, is an economically important nut crop. Propagation of pecan is done primarily by budding or grafting of improved cultivars onto seedling rootstocks. However, these methods suffer disadvantages such as considerable time, expense and poor transplanting survival of the plants. Propagation by cutting, though leads to uniform clonal rootstocks, is very difficult as the clones do not root readily. Even with the use of hormones, inconsistent results with only limited improvements have been reported (Brutsch et al. 1977;Smith et al. 1974;Wolstenholme and Allan 1975). In vitro propagation offers great potential for the pecan industry for large scale multiplication of selected clones and enables production of a high amount of constant quality plantlets within a very short time. Although somatic embryogenesis has been developed for pecan (Mathews and Wetzstein 1993;Merkle et al. 1987;Wetzstein et al. 1989Wetzstein et al. , 1990Yates and Reilly 1990), successful pecan micropropagation was not achieved because of poor regeneration and rooting. Wood (1982) and Knox and Smith (1981) have used nodal stem segment explants from seedlings to establish shoot proliferation, however, no consistent shoot proliferation, elongation or root development occurred and no plants were established in soil. Phillips and Ramirez (1983) and Corte-Olivares et al. (1990) used apical and axillary buds; shoot elongation was obtained, but rooting and establishment of plantlets were poor. Hansen and Lazarte (1984) used nodal cuttings as explants; shoots were rooted with 93% success and 63% plants were established in soil while Corte-Olivares et al. (1990) obtained 40% rooting of shoots. In this article, we report the development of a reliable and efficient protocol for rapid in vitro shoot induction and rooting of pecan plants by culturing nodal explants from seed in liquid culture. Although micropropagation of pecan through nodal explants from seed will result in heterozygous plants, the protocol optimized in this study could be applied for producing true-type plants from mature tissues.The unshelled mature seeds of pecan cultivars 'Cape Fear' and 'Desirable' were washed with sterile water for 10 min and then sterilized by immersion in the carbendazim solution (1.0 g l Ϫ1; Sigma Chemical Co., St. Louis, MO, USA) overnight. The seeds were washed with sterile water and treated with 70% ethanol for 30 min., again washed with sterile water for three times to remove ethanol. Subsequently, the seeds were treated with a 2.83% (w/v) sodium hypochlorite for 30 min. The seeds were thoroughly rinsed five times with sterile water and aseptically shelled using nut cracker. The embryos were aseptically isolated from the seeds and treated with 2.83% (w/v) sodium hypochlorite solution for 10 min. and rinsed with sterilized water five times for five min Abstract An efficient method for in vitro propagation has been developed for pecan [Carya illinoinensis (Wangenh) K. Koch], a hig...

show abstract

mentioning

confidence: 84%

“…The embryos were cultured modified woody plant medium (WPM with no glycine; Lloyd and McCown 1980;McCown and Lloyd 1981) 17.76 m M BAP and 0.49 m M IBA (Obeidy and Smith 1990) for three weeks for culture establishment. The medium was supplemented with sucrose (3% w/v) solidified with phytagel (0.35%, w/v, Sigma Chemical Co., St. Louis, MO, USA).…”

mentioning

confidence: 99%

In vitro propagation of pecan [Carya illinoinensis (Wangenh) K. Koch]

Renukdas

Manoharan

Garner

2010

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…Normal plants were obtained, without problems of contamination. Obeidy and Smith (1990) eliminated fungal contamination in mature pecan nuts by placing explants on a medium with low water availability (1.5% agar) for 30 days, followed by transfer to a medium with normal water status (0.7% agar). Disinfestation of up to 65 % of the cultures was accomplished, compared to 100% loss to contamination on control medium (0.5% agar).…”

Section: Organogenesismentioning

confidence: 99%