Establishing correlations in the en-mass migration of dermal fibroblasts on oriented fibrillar scaffolds

Qin, Sisi; Clark, Richard A.F.; Rafailovich, Miriam

doi:10.1016/j.actbio.2015.06.030

Cited by 10 publications

(11 citation statements)

References 63 publications

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“…Collective cell migration is essential in the wound healing process . The topography of nanofibers has a major impact on the migration and growth of cells involved in the wound healing process, including both keratinocytes and fibroblasts.…”

Section: Electrospun Nanofibers For Biomedical Applicationsmentioning

confidence: 99%

“…Collective cell migration is essential in the wound healing process. 864 The topography of nanofibers has a major impact on the migration and growth of cells involved in the wound healing process, including both keratinocytes and fibroblasts. Inspired by the basketweave-like pattern of collagen fibrils in the native skin, scaffolds made of crossed nanofibers were fabricated.…”

Section: Chemical Reviewsmentioning

confidence: 99%

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Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

et al. 2019

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Electrospinning is a versatile and viable technique for generating ultrathin fibers. Remarkable progress has been made with regard to the development of electrospinning methods and engineering of electrospun nanofibers to suit or enable various applications. We aim to provide a comprehensive overview of electrospinning, including the principle, methods, materials, and applications. We begin with a brief introduction to the early history of electrospinning, followed by discussion of its principle and typical apparatus. We then discuss its renaissance over the past two decades as a powerful technology for the production of nanofibers with diversified compositions, structures, and properties. Afterward, we discuss the applications of electrospun nanofibers, including their use as “smart” mats, filtration membranes, catalytic supports, energy harvesting/conversion/storage components, and photonic and electronic devices, as well as biomedical scaffolds. We highlight the most relevant and recent advances related to the applications of electrospun nanofibers by focusing on the most representative examples. We also offer perspectives on the challenges, opportunities, and new directions for future development. At the end, we discuss approaches to the scale-up production of electrospun nanofibers and briefly discuss various types of commercial products based on electrospun nanofibers that have found widespread use in our everyday life.

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Section: Electrospun Nanofibers For Biomedical Applicationsmentioning

confidence: 99%

Section: Chemical Reviewsmentioning

confidence: 99%

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

et al. 2019

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“…Cell migration was a highly integrated and complex process that was a fundamental empirical investigation in biomedical application research. Wound healing in vivo was dominated by multiple factors, among which the migration rate of the cell was likely to play a crucial role [42]. Cell migration was regulated by the local microenvironment, such as ECM rigidity, signaling factors and ligands on the ECM [43].…”

Section: Cell Migrationmentioning

confidence: 99%

Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype

Wang

Wang³

et al. 2022

J Leather Sci Eng

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Collagen made a tremendous impact in the field of regenerative medicine as a bioactive material. For decades, collagen has been used not only as a scaffolding material but also as an active component in regulating cells' biological behavior and phenotype. However, animal-derived collagen as a major source suffered from problems of immunogenicity, risk of viral infection, and the unclear relationship between bioactive sequence and function. Recombinant humanized collagen (rhCol) provided alternatives for regenerative medicine with more controllable risks. However, the characterization of rhCol and the interaction between rhCol and cells still need further investigation, including cell behavior and phenotype. The current study preliminarily demonstrated that recombinant humanized collagen type III (rhCol III) conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine collagen. Furthermore, rhCol III could facilitate basal biological behaviors of human skin fibroblasts, such as adhesion, proliferation and migration. rhCol III was beneficial for some extracellular matrix-expressing cell phenotypes. The study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration. Graphical abstract

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“…These materials could be formed by electrospinning methods. The main parameters of nonwoven electrospun materials include polymer composition, fiber diameter, degree of porosity, packing density, and orientation, all of which significantly affect the mechanical properties of the material [7][8][9][10][11] and the future success of scaffold implantation processes [12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

How the Nonwoven Polymer Volume Microstructure Is Transformed under Tension in an Aqueous Environment

et al. 2022

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The fibrous porous structure of polymers can mimic the extracellular matrix of the native tissue, therefore such polymers have a good potential for use in regenerative medicine. Organs and tissues within the body exhibit different mechanical properties depending on their functionality, thus artificial scaffolds should have mechanical behaviors similar to the extracellular matrix in conditions like living organisms, primarily in aqueous media. Several methods have been investigated in aquatic environments, including noninvasive techniques based on ultrasonic focused beams for biological objectives. In this study we explored the tensile behavior of poly(L-lactide) nonwoven polymer scaffolds using high-frequency ultrasound microscopy combined with a horizontal testing machine, which provided a visualization of the reorganization and transformation of the dynamic volume microstructure. The mechanisms of unwinding, elongation, orientation, and deformation of polymer fibers under uniaxial tension were revealed. We observed an association between the lined plastic deformation from 100 to 400% and the formation of multiple necks in the fibers, which caused stress relaxation and significant rarefaction of the fibrous microstructure. It was shown that both peaks on the stress–strain curve corresponded to the microstructure of aligned fibers in terms of initial diameter and thinning fibers. We discuss the possible influence of these microstructure transformations on cell behavior.

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Establishing correlations in the en-mass migration of dermal fibroblasts on oriented fibrillar scaffolds

Cited by 10 publications

References 63 publications

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

Electrospinning and Electrospun Nanofibers: Methods, Materials, and Applications

Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype

How the Nonwoven Polymer Volume Microstructure Is Transformed under Tension in an Aqueous Environment

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