2022
DOI: 10.3389/fpls.2022.900768
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Establishing Tetraploid Embryogenic Cell Lines of Magnolia officinalis to Facilitate Tetraploid Plantlet Production and Phenotyping

Abstract: The production of synthetic polyploids for plant breeding is compromised by high levels of mixoploids and low numbers of solid polyploid regenerants during in vitro induction. Somatic embryogenesis could potentially contribute to the maximization of solid polyploid production due to the single cell origin of regenerants. In the present study, a novel procedure for establishing homogeneous tetraploid embryogenic cell lines in Magnolia officinalis has been established. Embryogenic cell aggregate (ECA) about 100–… Show more

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Cited by 2 publications
(3 citation statements)
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“…On the other hand, the dominant bacterial abundance in the Panax seed microbiome was in agreement with the results from our previous study ( 16) on a series of wild plant seed microbes, where a high ratio of bacteria/fungi abundance was also found. The predominated bacterial endophytes have shown to have the ability to enhance seeding growth by increasing access to nutrients, such as by making iron more available, fixing nitrogen and solubilizing phosphate (Vendan et al, 2010;Hong et al, 2019;Liu et al, 2021;Gao et al, 2022;Goodwin, 2022). Such nutrients are essential to plant early-stage survival.…”
Section: Bacterial and Fungal Abundances And Composition In Panax Seedsmentioning
confidence: 99%
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“…On the other hand, the dominant bacterial abundance in the Panax seed microbiome was in agreement with the results from our previous study ( 16) on a series of wild plant seed microbes, where a high ratio of bacteria/fungi abundance was also found. The predominated bacterial endophytes have shown to have the ability to enhance seeding growth by increasing access to nutrients, such as by making iron more available, fixing nitrogen and solubilizing phosphate (Vendan et al, 2010;Hong et al, 2019;Liu et al, 2021;Gao et al, 2022;Goodwin, 2022). Such nutrients are essential to plant early-stage survival.…”
Section: Bacterial and Fungal Abundances And Composition In Panax Seedsmentioning
confidence: 99%
“…As found by Wei et al (2021), Panax endophytes seem to be transferred via the seed and then variously dominate in plant tissues; for instance, Proteobacteria and Firmicutes are known to be abundant in stems and roots, respectively (Tian et al, 2014). Apart from Firmicutes, Actinobacteria and Bacteroidetes are also Panax root-aggregating bacterial phyla that show considerable variation in abundance among studies (Hong et al, 2018a;Wei et al, 2021;Gao et al, 2022).…”
Section: Bacterial and Fungal Abundances And Composition In Panax Seedsmentioning
confidence: 99%
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