Establishment and application of a 10‐plex liquid bead array for the simultaneous rapid detection of animal species

Abstract

BACKGROUND: Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. RESULTS: This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg −1 in binary mixtures or 0.01-0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab. CONCLUSION: These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds.

Safety, Quality and Analytical Authentication of ḥalāl Meat Products, with Particular Emphasis on Salami: A Review

“…Moreover, often companies producing halāl meat products also process other kinds of meat, and thus cross-contaminations are possible. Therefore, many researches have been recently carried out for authenticating the species composition of meat products [ 87 , 88 , 89 , 90 , 91 ] and in particular the ḥalāl authentication studies are focused on the detection of pork derivatives (meat or lard) [ 92 , 93 ].…”

Molecular Sexing and Species Detection of Antlered European Hunting Game for Forensic Purposes

“…Multiplexing several primers together in one reaction mixture enables simultaneous identification of target species and improves the success rate of species detection when using only a minute amount of DNA. In contrast to the sequencing-based methods, combining species-specific primers with fragment length analysis, real-time PCR detection or multiplex hybridization assay allow the parallel identification of more species and can be applied to a DNA mixture [3,[46][47][48][49]. As many crime scenes are outdoor "living crime scenes", evidential samples are often exposed to environmental breakdown, and DNA shows considerable degradation over time, which can inhibit the subsequent DNA analysis.…”