Establishment and characterization of a mouse Schwann cell line which produces myelin in vivo

Abstract

A Schwann cell line (MSC 80) was established from purified mouse Schwann cell cultures using large doses of serum. MSC 80 cell line is an aneuploid cell line which has a doubling time of 17 hr and has been maintained through more than 110 passages. Most of MSC 80 cells are of bipolar or stellate (3-5 processes) shape. A few others are irregular in shape, gigantic, and multinucleated. All MSC 80 cells express antigens of myelin-forming Schwann cells such as S-100, 224/58, laminin, and other glycoproteins of the extracellular matrix. However, they also express the non-myelin-forming Schwann cell antigen GFAP. By time-lapse cinematography, MSC 80 cells exhibit the Schwann cell characteristic rhythmical undulations. When induced to form aggregates in agar, they form intercellular junctions and basement membrane-like structures. In addition, after transplantation in or at a distance from a lysolecithin induced lesion, MSC 80 cells form myelin around the host demyelinated axons. MSC 80 cells thus express, when isolated in vitro, some of the normal myelin-forming Schwann cell phenotype. In addition, they present the major advantage of forming myelin when associated with axons in vivo.

Wnt/β-Catenin Signaling Is an Essential and Direct Driver of Myelin Gene Expression and Myelinogenesis

“…The mouse Schwann cell line MSC80 exhibits normal Schwann cell characteristics [S100, myelin protein zero (MPZ), peripheral myelin protein 22 (PMP22) expression] and has retained the capacity to myelinate axons (Boutry et al, 1992;Baron-Van Evercooren et al, 1993). The 158N oligodendrocyte cell line originates from mature oligodendrocytes.…”

The Activation of the WNT Signaling Pathway Is a Hallmark in Neurofibromatosis Type 1 Tumorigenesis

“…MSC80 cells exhibit normal Schwann cell characteristics (S100, myelin protein zero, peripheral myelin protein 22 expression) and have retained the capacity to myelinate axons (27) Table S2). The mRNA expression of the 32 markedly differentially expressed genes was then determined in a larger tumor series including 10 dermal neurofibromas, 31 plexiform neurofibromas, and 16 MPNSTs (validation set: 57 tumors).…”

Phosphatidic acid mediates demyelination in Lpin1 mutant mice

“…MSC80 cell line The mouse Schwann cell line (MSC80) (Boutry et al 1992) was maintained in DMEM supplemented with 10% FCS, 100 U/mL penicillin, 100 µg/mL streptomycin, and 0.5 µg/mL fungizone (all reagents were from Invitrogen). In order to evaluate activation of either the MEK-Erk or PI3K-Akt pathways, the cells were grown for 1 h in the presence or absence of 100 µM phosphatidic acid (Sigma), PD98059 (20 µM; Cell Signaling), and LY294002 (10 µM; Cell Signaling).…”