Establishment and characterization of a new gastric cancer cell line, XGC-1

Abstract

Background: To establish a primary human gastric cancer cell line. Methods: Fresh gastric cancer tissue samples were separated into a cell suspension, and DMEM/F12 medium containing 10% foetal bovine serum was used for primary culture and subculture. The morphology of the cells was observed under a light microscope, and the cell growth curve was plotted. A soft agar colony formation assay was used to detect the colony formation ability of the cell line. Immunohistochemical methods were used to detect cytokeratin, vimentin and Ki-67, the chromosome G banding method was used to analyse the karyotype of the cells, and the tumourigenic ability of the cells was detected by subcutaneous inoculation of BALB/C nude mice. Results: We established a gastric cancer cell line from a 68-year-old male patient. This gastric cancer cell line was named XGC-1 and had a doubling time of approximately 48 h. The cell line displayed strong colony formation ability and tumourigenicity in BALB/C nude mice and had complicated chromosomal abnormalities. When nutrients were insufficient, the cells shed and floated in the medium, but adherent growth was observed in nutrient-rich conditions. Conclusions: The XGC-1 cell line will be useful for future studies of gastric cancer development, progression, metastasis and therapy.

Establishment of gastric cancer organoid and its application in individualized therapy

“…Differing from the organoid culture method including inoculation in Matrigel (28)(29)(30), the establishment of gastric cancer organoids by suspension culture has the following advantages: i) In the absence of Matrigel, the cost of the organoid culture may be markedly reduced; ii) in terms of collection, passage, cryopreservation and drug screening, the model is simpler and more convenient to handle; and iii) without Matrigel in the culture process, the impact of the interaction between mouse-derived components and human-derived tumors is reduced. Regarding the acquisition of primary cells, based on the literature and previous cell culture experience (28)(29)(30)(31)(32), it is recommended that during the enzymatic digestion process, tissue digestion is diligently observed and duly terminated in time. In this regard, cell mass-like structures rather than single cells may markedly improve the success rate of cell lines or organoids.…”

“…Previous studies by our group have indicated how fibroblast contamination may be reduced during organoid culture (31,32). First, the epithelial layer should be sampled as much as possible when the tumor tissue is obtained.…”

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

“…Therefore, more cell models derived from human primary tumors with clear backgrounds are required for the study of CRC biology. Like other established cell lines, the novel PUMC1-CRC cells have some particular advantages, including: high phenotypic heterogeneity, decreased genetic shifts, ideal complements for tissue banks, new tools for the evaluation of anticancer agents, and endless sources for genetic or proteomic analysis 25 – 27 .…”

HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer

“…XGC‐1 had been successfully established from GC patients diagnosed with IGC by Ming classification 15 . The XGC‐1, AGS and NUGC2 cell lines (Cell Bank of the Chinese Academy of Sciences) were cultured in RPMI‐1640 (Thermo Scientific) supplemented with 10% fetal bovine serum (Thermo Scientific) at 37°C with 5% CO 2 and 90%–95% humidity.…”