Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction

Abstract

Though human prostate cancer (PCa) heterogeneity can best be studied using multiple cell types isolated from clinical specimens, the difficulty of establishing cell lines from clinical tumors has hampered this approach. In this proof‐of‐concept study, we established a human PCa cell line from a prostatectomy surgical specimen without the need for retroviral transduction. In a previous report, we characterized the stromal cells derived from PCa specimens. Here, we characterized the epithelial cells isolated from the same tumors. Compared to the ease of establishing prostate stromal cell lines, prostatic epithelial cell lines are challenging. From three matched pairs of normal and tumor tissues, we established one new PCa cell line, HPE‐15. We confirmed the origin of HPE‐15 cells by short tandem repeat microsatellite polymorphism analysis. HPE‐15 cells are androgen‐insensitive and express marginal androgen receptor, prostate‐specific antigen and prostate‐specific membrane antigen proteins. HPE‐15 expresses luminal epithelial markers of E‐cadherin and cytokeratin 18, basal cell markers of cytokeratin 5 and p63 and neuroendocrine marker of chromogranin A. Interestingly, HPE‐15 Cells exhibited no tumorigenicity in different strains of immune‐deficient mice but can become tumorigenic through interaction with aggressive cancer cell types. HPE‐15 cells can thus serve as an experimental model for the study of PCa progression, metastasis and tumor cell dormancy.

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

“…Telomorase reverse transcriptase (TERT), SV40 T-antigen and HPV E6/E7 are often used to immortalize cells 32 – 37 . These tools can help us obtain unlimited experimental materials, while at the same time provide some artificial background 33 , 34 . As reported, intrinsic gene changes determine the successful establishment of stable cancer cell lines from tumor tissue 38 .…”

9p21 Loss Defines the Evolutionary Patterns of Aggressive Renal Cell Carcinomas

“…G-banded metaphase spreads were photographed using an 80i Nikon microscope and Applied Spectral Imaging karyotyping system (ASI, Vista, CA). A minimum of ten metaphases were karyotyped (35).…”

From Donor to the Lab: A Fascinating Journey of Primary Cell Lines

“…In that case, cell survival and tissue dissociations were the highest and the most satisfying (Volovitz et al, 2016). Isolation of primary cancer cells with dispase is also an effective procedure in prostate, ovarian and cervical cancer (Pribyl et al, 2014;Villa et al, 2018;Wang et al, 2019). The use of dispase enables the effective recovery of viable, fibroblast-free epithelial ovarian cancer cells (EOC).…”

“…For instance, prostate epithelial cells express various marker proteins during differentiation, thus their activity can be investigated by WB. It enables the characterisation of the molecular subtype of cell lines, including ones expressing CK18, CK 5, E-cadherin, or p63 (Wang et al, 2019). In ascending colon cancer, WB can be useful for the determination of the presence and size of insulinoma-associated protein 1 (INSM-1) from lysates.…”

“…Karyotypes are often used to investigate the aberrations in chromosome numbers, chromosomal translocations, deletions, and inversions (Sampson and McGuire, 2014). For this reason, karyotyping is a cheap and popular method of choice to demonstrate changes in the cell line and reveals exciting features of cell lines, that have never been observed before (Geraghty et al, 2014;Wang et al, 2019).…”