Establishment and characterization of a new cell line from the muscle of humpback grouper (Cromileptes altivelis)

Wang, Lu; Cao, Zhenjie; Liu, Yixuan; Xiang, Yajing; Sun, Yun; Zhou, Yongcan; Wang, Shifeng; Guo, Wei

doi:10.1007/s10695-020-00841-5

Cited by 27 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The similar results were also found in fibroblast SAF-1 cell line from S. aurata L. and muscle cell line from C. altivelis (Morcillo et al, 2016;Wang et al, 2020). The IC50 showed that the cytotoxicity of Hg (0.393mM) was more remarkable than that of Cd (0.445 mM) and Cu (1.073 mM) in CAB cells.…”

Section: Discussionsupporting

confidence: 80%

“…As previously mentioned, the cell cycle of CAB cells at passage 20 was analyzed using flow cytometry (Wang et al, 2020). Firstly, CAB cells were seeded and digested using trypsin after cultured for 24 h and 48 h, respectively.…”

Section: Cell Cycle Analysismentioning

confidence: 99%

“…Resuscitation test was performed as follows: CAB cells that stored in liquid nitrogen were taken out quickly and put it into a 37 °C water bath immediately for 80 s. Afterwards, cells were added into 25 cm 2 flasks with 4ml L-15 medium to culture. Finally, trypan blue test was conducted to analyze the cell viability as described by our previous study (Wang et al, 2020).…”

Section: Subculture Cryopreservation and Recovery Of Cellmentioning

confidence: 99%

See 2 more Smart Citations

Establishment of a New Fish Cell Line from the Brain of 1 Humpback Grouper (Chromileptes Altivelis), and its Application in Toxicology and Bacterial Susceptibility

Liu

Wei

Liu

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Cromileptes altivelis, humpback grouper, belongs to the family Epinephelidae and is one popular farmed fish species because of its high economic value and ornamental value. However, more and more diseases were outbreak with the increasing of C. altivelis breeding, resulting in severe economic losses. Today, a new brain cell line of C. altivelis (CAB) was established and has been subcultured for more than 40 passages until now. Our results showed that the morphology of CAB cells is epithelial-like and suitable for growth at 26 °C in L15 medium supplemented with 15% fetal bovine serum (FBS). The results of 18S rRNA gene sequencing confirmed that CAB cell line was derived from C. altivelis. Moreover, CAB cells were apparently diploid number of chromosomes (2n = 48) by chromosome analysis. In addition, CAB cells could transfect pEGFP-N3 plasmid with high transfection efficiency, indicating that CAB cell line has the potential to investigate the function of exogenous genes in vitro. Furthermore, the bacterial susceptibility results suggested that CAB cells were susceptive to Vibrio harveyi and Edwardsiella tarda. And, heavy metals (Hg, Cd, and Cu) were toxic to the CAB cells and the toxic effect was dose-dependent. In summary, the CAB cell line could be a powerful tool in vitro to study functional genes and has the potential application in bacterial susceptibility and toxicology.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Cell Cycle Analysismentioning

confidence: 99%

Section: Subculture Cryopreservation and Recovery Of Cellmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of a New Fish Cell Line from the Brain of 1 Humpback Grouper (Chromileptes Altivelis), and its Application in Toxicology and Bacterial Susceptibility

Liu

Wei

Liu

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the SCC cell line, the normal diploid chromosome number of the SCC cells at passage 30 was 48, same as the reported chromosome number for S. chuatsi (Dong et al, 2008), as well as the cell lines derived from this species (Fu et al, 2015). Mycoplasma contamination is a major problem in cell culture (Jin et al, 2017;Xue et al, 2018) and preventing mycoplasma contamination is extremely important for cells that are difficult to obtain or particularly valuable (Wang et al, 2020). Previous studies (Yadav et al, 2021) have shown that mycoplasma contamination not only affects the production of virus, but also has the potential to alter cell membrane antigenicity, resulting in cell death.…”

Section: Discussionsupporting

confidence: 70%

“…Mycoplasma contamination is a major problem in cell culture (Jin et al, 2017; Xue et al, 2018) and preventing mycoplasma contamination is extremely important for cells that are difficult to obtain or particularly valuable (Wang et al, 2020). Previous studies (Yadav et al, 2021) have shown that mycoplasma contamination not only affects the production of virus, but also has the potential to alter cell membrane antigenicity, resulting in cell death.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Gong

Lin

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

Siniperca chuatsi, a mandarin fish, is a unique species of freshwater fish in China. It is one of the most economically important freshwater fish due to its delicious taste and high nutritional value. In 2019, the total farmed production of S. chuatsi in China was nearly 320,000 tons, with an output value exceeding RMB 20 billion (Li et al., 2021). Mandarin fish are mainly farmed in Guangdong, Hubei, Jiangxi, Anhui and Jiangsu Provinces of China, with the highest production in Guangdong. The expansion of the scale and density of mandarin fish farming has led to a variety of pathogenic microorganisms causing disease outbreaks in mandarin fish (Sun et al., 2017). A number of bacterial, parasitic and viral diseases have been reported from the mandarin fish. Among the viruses causing infectious diseases in fish, the highly infectious and lethal viruses Infectious Spleen and Kidney Necrosis Virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV), have been reported in the mandarin fish and cause considerable losses to mandarin fish aquaculture. For example, SCRV outbreaks in hundreds of fish farms in Guangdong were reported, which caused death of more than 10 million juvenile mandarin fish (Zhang et al., 2020). Therefore, the prevention and treatment of these viral diseases has become an urgent problem to be solved in the mandarin fish aquaculture.

show abstract

Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi)

Jing,

Mei,

et al. 2024

Arch Virol

View full text Add to dashboard Cite

Establishment and characterization of a new cell line from the muscle of humpback grouper (Cromileptes altivelis)

Cited by 27 publications

References 53 publications

Establishment of a New Fish Cell Line from the Brain of 1 Humpback Grouper (Chromileptes Altivelis), and its Application in Toxicology and Bacterial Susceptibility

Establishment of a New Fish Cell Line from the Brain of 1 Humpback Grouper (Chromileptes Altivelis), and its Application in Toxicology and Bacterial Susceptibility

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi)

Contact Info

Product

Resources

About