Establishment and characterization of an inverted papilloma–associated sinonasal squamous cell carcinoma cell line

Swenson, Wade T.; Miller, Kimberly A.; Wuertz, Beverly R.; Jungbauer, W. Nicholas; Ondrey, Frank G.

doi:10.1002/alr.22739

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…7 B,C). Our newly established cell cultures appear to exhibit less genetic alteration than do other previously established SIP-associated SNSCC cells 49 , 57 .…”

Section: Discussionmentioning

confidence: 57%

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Nukpook

Ekalaksananan

Kiyono

et al. 2021

Sci Rep

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To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents.

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“…7 B,C). Our newly established cell cultures appear to exhibit less genetic alteration than do other previously established SIP-associated SNSCC cells 49 , 57 .…”

Section: Discussionmentioning

confidence: 57%

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Nukpook

Ekalaksananan

Kiyono

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

Experimental Models of Sinonasal Tumors for Preclinical Testing of Candidate Targeted Therapies

Codina-Martínez,

Lorenzo-Guerra,

Cabal

et al. 2023

Curr Otorhinolaryngol Rep

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EUSICA/COST IMMUNO-model workshop fostering collaboration to advance sinonasal cancer research: A meeting report

et al. 2023

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Establishment and characterization of an inverted papilloma–associated sinonasal squamous cell carcinoma cell line

Abstract: papilloma inverted squamous carcinoma squamous cell line SSC How to Cite this Article Swenson W Miller KA Wuertz B et al Establishment and characterization of an inverted papilloma-associated sinonasal squamous cell carcinoma cell line Int Forum Allergy Rhinol https //doi org/ /alr

Cited by 3 publications

References 9 publications

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Experimental Models of Sinonasal Tumors for Preclinical Testing of Candidate Targeted Therapies

EUSICA/COST IMMUNO-model workshop fostering collaboration to advance sinonasal cancer research: A meeting report

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