Establishment and characterization of embryogenic cell suspension cultures from immature and mature embryos of barley (Hordeum vulgare L.)

Abstract

Two finely-dispersed, homogeneous and regenerable cell suspension cultures were established from embryogenic callus derived from immature and mature embryos in barley. The quality and viability of suspension cells obtained were determined using differential-interference-contrast microscope and fluorescence microscope. Cell suspension cultures, maintained in modified liquid CC medium, showed a 10-fold increase in dry weight after two weeks with a doubling time of about 3 days. Addition of L-proline and casein hydrolysate in the medium had positive effect on the growth of cell cultures. Subculture interval significantly affected mitotic index. Both cell lines established were able to regenerate plants by somatic embryogenesis, but cell line Z-IM showed much higher regeneration capacity than cell line Z-M. Comparatively high frequencies of variations in chromosome number and structure were found in both lines, and a correlation between karyotype and morphogenic capacity was noticed.

Somatic embryogenesis and fertile green plant regeneration from suspension cell-derived protoplasts of rye ( Secale cereale L.)

“…Eight of 30 initiated suspensions in AA medium, and four of 30 suspensions in mMS medium were established in our experiment. Weekly changes of medium, which maintained the suspensions in exponential growth, were in agreement with previous results mentioned for rice ), wheat (Vasil et al 1990) and barley (Huang et al 1993).…”

“…It was found that the two steps used for rice (Jenes and Pauk 1989), wheat (Harris et al 1988;Wang et al 1990) and barley (Huang et al 1993) were necessary for plant regeneration of rye. In the first step, protocalli were transferred to mMS growth medium (supplemented with 0.5 mg l -1 2,4-D) for compact embryogenic calli development.…”

“…Suitable embryogenic calli are a prerequisite for the establishment of such a suspension. Embryogenic callus induction and selection procedures have been commonly employed in protoplast culture of rice Utomo et al 1995), wheat (Harris et al 1988;Vasil et al 1990;Li et al 1992) and barley (Lührs and Lörz 1988;Huang et al 1993). In our experiments, embryogenic calli were induced from young inflorescences cultured on mMS medium.…”

Cytogenetic variation in somatic tissue cultures and regenerated plants of barley (Hordeum vulgare L.)

“…However, Singh (1986) and Gaponenko et al (1988) observed that diploid cells predominated in morphogenic calli obtained from immature barley embryo while polyploid and aneuploid cells increased significantly or even predominated when calli became non-morphogenic. A correlation between chromosomal variability and morphogenic capability in barley embryogenic suspensions (Huang et al, 1993) has also been indicated.…”

Effects of l-Proline and Cold Treatment on Pepper (Capsicum annuum L.) Anther Culture

“…It has been shown that the addition of activated charcoal [1,25,24,16], inclusion of different concentration of L-proline in the culture medium [5,4,9,19,7] and cold pretreatment of whole buds or anthers [12] or different concentrations of NAA and BA combination [20,21,18] could increase embryo production. Beneficial effects of temperature shock treatments on anther culture productivity were known.…”