Establishment and characterization of lymphoid and myeloid mixed-cell populations from mouse late embryoid bodies, "embryonic-stem-cell fetuses".

Chen, Una; Kosco, Marie H.; Staerz, Uwe D.

doi:10.1073/pnas.89.7.2541

Cited by 42 publications

(19 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a similar approach, lymphocytes were generated following injection of heterozygous mutant ES cells into blastocysts from homozygous scid/scid mice (25); however, this system was not explored in detail and may be compromised by the leakiness and pleiotropic nature of scid mutation (26,27). Recently, several groups have reported success in differentiating ES cells into lymphocyte precursors in culture (28,29). When perfected, such methods could be particularly advantageous for examining early stages of lymphoid development.…”

Section: Discussionmentioning

confidence: 99%

RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.

Chen

Lansford

Stewart

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

365

269

View full text Add to dashboard Cite

We describe a system to evaluate the function of lymphocyte-specific and generafly expressed genes in the differentiation and/or function of lymphocytes. RAG-2 (recombination-activating gene 2)-deficient mice have no mature B and T lymphocytes due to the inability to initiate VDJ recombination. Blastocysts from RAG-2-deficient mice generate animals with no mature B and T cells foOlowing implantation into foster mothers. However, i 'ection of normal ES

show abstract

Section: Discussionmentioning

confidence: 99%

RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.

Chen

Lansford

Stewart

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

365

269

View full text Add to dashboard Cite

show abstract

“…This specific regulation of iNOS mRNA occurred during the spontaneous differentiation induction of the cells in control medium, which is induced by removal of LIF. During the first five differentiation days, which is before the addition of VD3, progenitors of all three germ layers are specified (Chen et al, 1992) a subpopulation of which then responds to the osteogenic induction (zur Nieden et al, 2003).…”

Section: Temporal Expression Pattern Of No Pathway Membersmentioning

confidence: 99%

NO/beta-catenin crosstalk modulates primitive streak formation prior to embryonic stem cell osteogenic differentiation

Ding

Keller

Martinez

et al. 2012

Journal of Cell Science

View full text Add to dashboard Cite

SummaryNitric oxide (NO) has been shown to play a crucial role in bone formation in vivo. We sought to determine the temporal effect of NO on murine embryonic stem cells (ESCs) under culture conditions that promote osteogenesis. Expression profiles of NO pathway members and osteoblast-specific markers were analyzed using appropriate assays. We found that NO was supportive of osteogenesis specifically during an early phase of in vitro development (days 3-5). Furthermore, ESCs stably overexpressing the inducible NO synthase showed accelerated and enhanced osteogenesis in vitro and in bone explant cultures. To determine the role of NO in early lineage commitment, a stage in ESC differentiation equivalent to primitive streak formation in vivo, ESCs were transfected with a T-brachyury-GFP reporter. Expression levels of T-brachyury and one of its upstream regulators, b-catenin, the major effector in the canonical Wnt pathway, were responsive to NO levels in differentiating primitive streak-like cells. Our results indicate that NO may be involved in early differentiation through regulation of b-catenin and T-brachyury, controlling the specification of primitive-streak-like cells, which may continue through differentiation to later become osteoblasts.

show abstract

“…Total RNA was extracted as described above. cDNA was synthesized from 1 g of total RNA, with a random hexamer [pd(N) 6 ] as primer (Pharmacia Biotech) and Moloney murine leukemia virus RT (Gibco BRL). cDNA samples were subjected to PCR amplification with specific primers.…”

Section: Cells and Media Cce Es Cellsmentioning

confidence: 99%

Involvement of Hepatocyte Nuclear Factor 3 in Endoderm Differentiation of Embryonic Stem Cells

Levinson-Dushnik

Benvenisty

1997

Molecular and Cellular Biology

116

View full text Add to dashboard Cite

The transcription factors of the hepatocyte nuclear factor 3 (HNF3) family, which are active in the liver, are expressed early during endoderm differentiation. To study their involvement in early murine development, we examined their role in embryonic stem (ES) cells. HNF3␣ or HNF3␤ mRNA transcripts were not detected in ES cells before differentiation, and only low levels of HNF3␤ mRNA were detected at a late stage of differentiation of ES cells to embryoid bodies (EB) (20 days after induction of differentiation). To examine the consequences of overexpressing HNF3␣ or -␤ in ES cells, we transfected the two genes into these cells and determined the levels of expression of tissue-specific genes during EB differentiation. Specifically, we examined expression of albumin, cystic fibrosis transmembrane conductance regulator (CFTR), phosphoenolpyruvate carboxykinase (PEPCK), ␣1-antitrypsin, transthyretin, -globin, and neurofilament 68kd as markers for different cell lineages. Overexpression of HNF3␤ (and to a lesser extent of HNF3␣) induced the expression of genes associated with endodermal lineage, namely, the genes for CFTR and albumin, but did not induce the expression of genes involved in late endoderm differentiation, such as the genes for PEPCK and ␣1-antitrypsin. Moreover, expression of HNF1␤ was highly induced in HNF3-overexpressing cells, while expression of HNF1␣ and HNF4 was only mildly induced in these cells. Therefore, HNF3␣ and -␤ seem to be involved in early endoderm differentiation of ES cells and together with other developmental factors are apparently needed for the induction of the endodermal lineage in vivo.The study of cell-specific transcriptional control has led to the identification of transcription factors which are enriched in hepatocytes, among them the hepatocyte nuclear factor 3 (HNF3) family (consisting of HNF3␣, -␤, and -␥) (42). The HNF3 proteins were first identified in rat liver nuclear extracts by virtue of their ability to bind specifically to sequences required for hepatocyte-specific expression of mouse genes (15). HNF3␤, -␣, and -␥ are sequentially activated during the development of the definitive endoderm (42). HNF3␤ mRNA is expressed in the node at the anterior end of the primitive streak in all three germ layers and is the first gene of this family to be activated (21). Subsequently, HNF3␣ is transcribed in the primitive endoderm in the region of the invaginating foregut, and HNF3␥ appears upon hindgut differentiation (21). HNF3␣ and -␤ are required for mesoderm and neural axis formation, as well as for liver-specific gene expression (21).Embryonic stem (ES) cells are clonal cells derived from the preimplantation mouse embryo (24). ES cells can multiply in culture and remain pluripotent. There are three main mechanisms by which ES cells can be induced to undergo differentiation to all cell types. First, when injected into the inner cell mass of a host blastocyst, they integrate into the inner cell mass and populate all cell lineages including the germ line. Second, when inject...

show abstract

Establishment and characterization of lymphoid and myeloid mixed-cell populations from mouse late embryoid bodies, "embryonic-stem-cell fetuses".

Cited by 42 publications

References 46 publications

RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.

RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.

NO/beta-catenin crosstalk modulates primitive streak formation prior to embryonic stem cell osteogenic differentiation

Involvement of Hepatocyte Nuclear Factor 3 in Endoderm Differentiation of Embryonic Stem Cells

Contact Info

Product

Resources

About