Establishment and characterization of rat progenitor hair cell lines

Ozeki, Masashi; Duan, Lijie; Hamajima, Yuki; Obritch, Wesley; Edson-Herzovi, Diana; Lin, Jizhen

doi:10.1016/s0378-5955(03)00077-7

Cited by 25 publications

(30 citation statements)

References 49 publications

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“…OC1 is a cochlear sensory epithelial cell line from a postnatal Day 5 rat organ of Corti [16]. The study was approved by the Institutional Animal Care and Use Committee (IACUC).…”

Section: Methodsmentioning

confidence: 99%

“…The specific primers for the EGFR (5'-agtggtccttggaaacttgg-3'/5'-gttgacatccatctggtacg-3'), n-ras (5'-gaaagagggaactgaaacgatt-3'/5'-taggctgaaaga gagggatggt-3'), c-raf (5'-gggaaatagaagccagtg aggt-3'/5'-caattagctggaacatctggaa-3'), Bcl-2 (5'-gacctctgtttgatttctcc-3'/ 5'-tggtccatccttgataatgc-3') were designed with Oligo 4.0, and the specificity of the primers was confirmed by the BLAST search engine provided by the National Center of Biologic Information website (fttp://www.ncbi.nlm.nih.gov). RT-PCR was performed as described previously [16] using ribosomal protein S29 as a loading control. PCR products, routinely sequenced, were analyzed on a 2% agarose gel.…”

Section: Rt-pcrmentioning

confidence: 99%

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EGF Mediates Survival of Rat Cochlear Sensory Cells via an NF-κB Dependent Mechanism In Vitro

Zheng

Rayner²,

Ling³

et al. 2008

TONEURJ

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Abstract:The survival of cochlear epithelial cells is of considerable importance, biologically. However, little is known about the growth factor(s) that are involved in the survival of cochlear sensory epithelial cells. In this study, we demonstrated that epidermal growth factor (EGF) plays a role in the survival of cochlear epithelial cells. Firstly, the presence of the EGF signaling pathway was demonstrated in the developing cochlear tissues of rats and a sensory epithelial cell line (OC1): --epidermal growth factor receptor (EGFR), mitogen-activated protein kinase kinase (MAPKK), I kappa B alpha (I B ), nuclear factor kappa B (NF-B), and B cell lymphoma 2 (Bcl-2). Secondly, the addition of EGF to OC1 increased the promoter activity of NF-B and cell viability but not cell cycle progression and cell number increase --which suggests that EGF is for cellular survival rather than cell proliferation of OC1. Finally, pyrrolidine dithiocarbamate (PDTC, an inhibitor of NF-B) and inhibitor kappa B alpha (I B ) mutant (I B M, a specific inhibitor of NF-B) abrogated the EGFinduced NF-B activity and cell survival. These data suggest that EGF plays a role in the survival of cochlear sensory epithelial cells through the EGFR/MAPKK/I B /NF-B/Bcl-2 pathway.

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“…OC1 is a cochlear sensory epithelial cell line from a postnatal Day 5 rat organ of Corti [16]. The study was approved by the Institutional Animal Care and Use Committee (IACUC).…”

Section: Methodsmentioning

confidence: 99%

Section: Rt-pcrmentioning

confidence: 99%

EGF Mediates Survival of Rat Cochlear Sensory Cells via an NF-κB Dependent Mechanism In Vitro

Zheng

Rayner²,

Ling³

et al. 2008

TONEURJ

Self Cite

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“…However, SV40 tsA58 TAg is inactivated when cultured at 39 0 C in the absence of interferon. Procedures for procurement of cells from the postnatal organ of Corti were performed as previously described (14), with some modifications. Briefly, two organ of Corti specimens from one P1 Immortomouse was dissected out from the both cochleas under an operating microscope and placed into 1 mL of growth media.…”

Section: Isolation and Maintenance Of Cnpsmentioning

confidence: 99%

“…RT-PCR was performed as described previously (14,15). PCR products were analyzed on a 2% agarose gel and routinely purified for sequence verification.…”

Section: Characterization Of Primitive Cnpsmentioning

confidence: 99%

“…From the 5 th passage culture of primitive CNPs after enrichment, approximately 30 single cells were diluted in 2 mL of growth media, divided into 90 wells (200 μL per well) of a 96-well plate, and cultured in growth media until appearance of cell clones, as previously described (14). Growth of single clones was examined under a contrast microscope on a daily basis.…”

Section: Clonal Cnps and Their Proliferation Featuresmentioning

confidence: 99%

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Differentiation of cochlear neural progenitors with SV40 in vitro

et al. 2009

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Ventral otic cell lines as developmental models of auditory epithelial and neural precursors

Lawoko-Kerali¹,

Milo²,

Davies

et al. 2004

Developmental Dynamics

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Conditionally immortal cell lines were established from the ventral otocyst of the Immortomouse at embryonic day 10.5 and selected to represent precursors of auditory sensory neural and epithelial cells. Selection was based upon dissection, tissue-specific markers, and expression of the transcription factor GATA3. Two cell lines expressed GATA3 but possessed intrinsically different genetic programs under differentiating conditions. US/VOT-E36 represented epithelial progenitors with potential to differentiate into sensory and nonsensory epithelial cells. US/VOT-N33 represented migrating neuroblasts. Under differentiating conditions in vitro the cell lines expressed very different gene expression profiles. Expression of several cell- and tissue-specific markers, including the transcription factors Pax2, GATA3, and NeuroD, differed between the cell lines in a pattern consistent with that observed between their counterparts in vivo. We suggest that these and other conditionally immortal cell lines can be used to study transient events in development against different backgrounds of cell competence.

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Establishment and characterization of rat progenitor hair cell lines

Cited by 25 publications

References 49 publications

EGF Mediates Survival of Rat Cochlear Sensory Cells via an NF-κB Dependent Mechanism In Vitro

EGF Mediates Survival of Rat Cochlear Sensory Cells via an NF-κB Dependent Mechanism In Vitro

Differentiation of cochlear neural progenitors with SV40 in vitro

Ventral otic cell lines as developmental models of auditory epithelial and neural precursors

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