Establishment and identification of a rabbit model of peritoneal carcinomatosis from gastric cancer

Mei, Lie‐Jun; Yang, Xiaojun; Tang, Li; Hassan, Alaa Hammed al-shammaa; Yonemura, Yutaka; Li, Yan

doi:10.1186/1471-2407-10-124

Cited by 22 publications

(18 citation statements)

References 51 publications

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“…Therefore, the appropriate inoculation concentration was determined to be 1×10 7 cells/well. These observations were similar to those identified previously ( 25 , 26 ), which provided a valuable insight into the establishment of animal gastric cancer models.…”

Section: Discussionsupporting

confidence: 90%

Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer

Zhang

Mao

et al. 2014

Oncology Letters

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Gastric cancer is the fourth most common type of malignant tumor, with a poor prognosis. Focal adhesion kinase (FAK) mediates the crosslink of intracellular signaling networks, playing a key role in cell migration and invasion. The aim of the present study was to investigate the effects of FAK interference on the proliferation ability, invasion and metastasis of gastric cancer cells. The FAK-RNAi lentiviral vector was infected into SGC7901 gastric cancer cells in order to observe the in vivo situations of tumor growth and metastasis before and after the FAK interference. The growth of SGC7901 gastric cancer cells in the interference group was significantly inhibited compared with that of the negative control (P<0.05) and the blank control groups (P<0.05), and the FAK expression significantly decreased (P<0.05). The in vitro invasion and metastasis experiments showed that the cell invasion and metastasis abilities of the interference group significantly decreased when compared with those of the negative control (P<0.05) and blank control groups (P<0.05). In the nude mouse subcutaneous tumor transplantation model, the mean ± standard deviation tumor weight of the interference group (1.474±0.9840 g) was lower than that of the negative control (3.134±0.3299 g) and blank control (2.68±0.12 g) groups (P<0.05). In the nude mice, the liver and peritoneal metastasis rates of the interference group were significantly lower than those of the negative control (P<0.05) and the blank control groups (P<0.05), and the FAK mRNA of the interference group significantly reduced (P<0.05). In conclusion, FAK interference could effectively suppress the proliferation, invasion and metastasis of transfected SGC7901 gastric cancer cells, and could inhibit the growth and distant metastasis of gastric cancer in nude mice.

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Section: Discussionsupporting

confidence: 90%

Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer

Zhang

Mao

et al. 2014

Oncology Letters

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“…A number of i.p. carcinomatosis mouse models had been established to study the processes of peritoneal dissemination and to verify the effects of novel therapeutic approaches since the first report of subcutaneous (s.c.) heterologous growth of human ovarian cancer tissue in nude mice ( 7 , 8 ). In a number of these studies, human tumor cells were used for s.c injection.…”

Section: Introductionmentioning

confidence: 99%

Establishment and characterization of intraperitoneal xenograft models by co-injection of human tumor cells and extracellular matrix gel

Yao

Zhou

et al. 2015

Oncology Letters

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Establishing a feasible intraperitoneal (i.p.) xenograft model in nude mice is a good strategy to evaluate the antitumor effect of drugs in vivo. However, the manipulation of human cancer cells in establishing a stable peritoneal carcinomatosis model in nude mice is problematic. In the present study, the ovarian and colorectal peritoneal tumor models were successfully established in nude mice by co-injection of human tumor cells and extracellular matrix gel. In ovarian tumor models, the mean number tumor nodes was significantly higher in the experimental group (intraperitoneal tumor cell co-injection with ECM gel) compared with the PBS control group on the 30th day (21.0±3.0 vs. 3.6±2.5; P<0.05). The same results were observed in the colorectal peritoneal tumor models on the 28th day. The colorectal peritoneal tumor model was further used to evaluate the chemotherapy effect of irinotecan (CPT-11). The mean weight of peritoneal tumor nodes in CPT-11 treatment group was significantly less than that of the control group (0.81±0.16 vs. 2.18±0.21 g; P<0.05). The results confirmed the value of these i.p. xenograft models in nude mice as efficient and feasible tools for preclinical evaluation.

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“…Rabbit VX2 cancer models of the liver, kidney, lung and bone have been studied maturely, and have been widely applied for use in tumor imaging, physiological and pathological studies, as well as in the efficacy evaluation of interventional treatment (14). However, VX2 brain tumor animal models have been rarely used in domestic and foreign studies, and the tumor implantation method used is primarily the intracerebral injection of tumor cell suspension (15,16). At the start of the present study, a number of other methods had been attempted; however, various shortcomings were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, the tumor size and shape were found to differ substantially between models. Based on the results of a previous study, the model preparation method was improved (15). Firstly, the cell suspension was replaced with a tumor block for implantation, as the block size can be more easily quantified compared with the tumor cells.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging-navigated argon-helium cryoablation therapy against a rabbit VX2 brain tumor

Wang

Kan

Sun

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the feasibility of interventional magnetic resonance imaging (MRI)-guided and monitored argon-helium cryoablation for the treatment of brain tumors in rabbits. In addition, the present study evaluated the associations between imaging and pathology, the therapeutic effects and the effects on the surrounding normal tissues. A total of 14 rabbits were equally divided into groups C and D. Under general anesthesia, the skull was drilled and tumor blocks were implanted. Subsequently, a New Zealand rabbit VX2 brain tumor model was successfully established. Rabbits in group C were treated with argon-helium cryoablation and those in group D did not undergo any treatment (control). Regular postoperative MRI scanning was performed to observe the changes in tumor size, and the survival times of the rabbits in groups C and D were recorded. The extent of necrosis in the brain tumor exhibited a significant correlation with the freezing time of cryoablation, and the necrotic region was shown to be the same size as the ice ball. The survival times of the rabbits in the treatment group (group C) were significantly prolonged. Therefore, the observations of the present study demonstrated that the VX2 brain tumor model, produced using an improved tumor block implantation method, was stable and suitable for MRI observation and interventional study. In addition, argon-helium cryoablation was shown to be a safe and feasible therapeutic method for the treatment of brain tumors, and was demonstrated to significantly increase the survival times of the brain tumor-bearing rabbits. IntroductionPrimary brain tumors have a high incidence rate, and tumor growth is invasive with an obscure boundary (1). Although brain tumors can be resected through various treatment methods, including surgery, radiotherapy and chemotherapy, disease progression is unable to be effectively controlled. In addition, the prognosis of patients with a brain tumor is usually poor, with a short survival time and severe sequelae (2).Magnetic resonance imaging (MRI)-navigated argonhelium cryoablation is a new advanced technology. MRI is known to have good tissue resolution and a multiplanar imaging capability. In particular, the temperature-sensitive characteristics of MRI, which enable the clear and real-time display of the size and range of the ice ball (which destroys the tumor cells), has resulted in this imaging technique becoming the focus in clinical cryotherapy against multiple-system tumors, with satisfactory results previously achieved (3-7). Although MRI has an unparalleled advantage in nervous system imaging compared with other methods (8), there are a limited number of domestic and foreign studies investigating MRI-navigated cryotherapy for the treatment of brain tumors.In the present study, a rabbit VX2 brain tumor model was established that was suitable for MRI imaging and interventional therapy. The model was used for the preliminary study of MRI-navigated argon-helium cryoablation therapy against t...

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Establishment and identification of a rabbit model of peritoneal carcinomatosis from gastric cancer

Cited by 22 publications

References 51 publications

Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer

Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer

Establishment and characterization of intraperitoneal xenograft models by co-injection of human tumor cells and extracellular matrix gel

Magnetic resonance imaging-navigated argon-helium cryoablation therapy against a rabbit VX2 brain tumor

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