Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus

Donofrío, Gaetano; Cavirani, S.; Santen, Vicky L. van

doi:10.1099/0022-1317-81-7-1807

Cited by 23 publications

(20 citation statements)

References 43 publications

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“…In addition, preliminary studies have shown that some human cell lines support BoHV-4 replication, leading to the hypothesis that this virus could represent a risk to human health [12,16]. The frequent isolation of BoHV-4, alone or in combination with other pathogens, from the genital tract of cows with metritis [17,18] has suggested a direct or indirect role of the virus in genital disease. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599-like strains (American prototype), and Genotype 3 corresponds to a novel genotype group [19].…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)

et al. 2016

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Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599-like strains (American prototype) and Genotype 3 corresponds to a novel genotype group. Understanding the replication behavior in cell cultures and the molecular characteristics of this pathogen of cattle is critical for the rational design of in vitro experiments. The aim of this work was to quantitatively evaluate the replication properties of different Argentinean BoHV-4 strains and to characterize their phylogenetic relationships. Significant differences were evident among the virus titers of the different BoHV-4 isolates in vitro. The most conserved gene was the major capsid protein (ORF25). The glycoprotein B (gB), glycoprotein H (gH), and thymidine kinsase (TK) genes displayed both synonymous and non-synonymous substitutions, with the highest diversity observed for gB, which displayed amino acid substitutions in 24 out of the 178 positions examined. Strains 09/759, 12/512, and 07/568 presented a deletion encompassing amino acid position 27 to 35, whereas strains 07/435 and 09/227 had a deletion from position 28 to 35. Two strains, 07/435 and 09/227, also displayed the highest divergence compared to the other strains analyzed. This study provides information about the in vitro replication and behavior of nine field isolates of BoHV-4. These findings are relevant since available information on the in vitro growth characteristics of BoHV-4 strains is scarce. The results from this study may also be useful for establishing comparisons with other related viruses.

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Section: Introductionmentioning

confidence: 99%

Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)

et al. 2016

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“…Second, many facts enable the transmission of the virus from cattle to humans: (i) infected animals excrete BoHV-4 in nasal and vaginal discharges both after primary infection and after reactivation (54), making human contamination possible for people having contact with infected cattle; (ii) BoHV-4 has been found in the milk of cows with mastitis (13,62,63), as well as from apparently healthy cows, suggesting possible human contamination by the oral route; and (iii) BoHV-4 is frequently isolated from bovine serum (7), which is used abundantly in food and pharmacological preparations, making human contamination by BoHV-4 possible by enteral or parenteral routes. Third, as mentioned above, earlier studies have revealed the capacity of some human cell lines to support BoHV-4 replication, suggesting the ability of the virus to infect humans (11,12,17).…”

mentioning

confidence: 99%

“…In vitro, BoHV-4 is able to replicate in primary cell cultures or cell lines from various animal species such as cattle, sheep, goats, swine, cats, dogs, rabbits, minks, horses, turkeys, ferrets, chickens, hamsters, rats, mice, and monkeys (1,2,11,17,20,32,33,47,53). In addition, preliminary studies have shown that some human cell lines support BoHV-4 replication, leading to the hypothesis that this virus could represent a risk to human health (11,12,17).…”

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confidence: 99%

Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis

Gillet¹,

Minner²,

Detry³

et al. 2004

J Virol

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Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that has a worldwide distribution in the population of cattle. Many factors make human contamination by BoHV-4 likely to occur. In this study, we performed in vitro experiments to assess the risk and the consequences of human infection by BoHV-4. First, by using a recombinant BoHV-4 strain expressing enhanced green fluorescent protein under the control of the human cytomegalovirus immediate-early gene promoter, we tested 21 human cell lines for their sensitivity and their permissiveness to BoHV-4 infection. These experiments revealed that human cell lines from lymphoid and myeloid origins were resistant to infection, whereas epithelial cells, carcinoma cells, or adenocarcinoma cells isolated from various organs were sensitive but poorly permissive to BoHV-4 infection. Second, by using the HeLa cell line as a model of human cells sensitive but not permissive to BoHV-4 infection, we investigated the resistance of infected cells to apoptosis and the persistence of the infection through cellular divisions. The results obtained can be summarized as follows. (i) BoHV-4 nonpermissive infection of HeLa cells protects them against tumor necrosis factor alpha-induced apoptosis. (ii) BoHV-4 infection of HeLa cells persists in cell culture; however, the percentage of infected cells decreases with time due to erratic transmission of the viral genome through cell division. (iii)BoHV-4 infection has no effect on the rate of HeLa cell division. Altogether, these data suggest that BoHV-4 could infect humans. This study also stresses the importance of considering the insidious effects of nonpermissive infection when the biosafety of animal gammaherpesviruses for humans is being considered.Bovine herpesvirus 4 (BoHV-4) belongs to the Herpesviridae family, the Gammaherpesvirinae subfamily, and the Rhadinovirus genus (66). BoHV-4 has been isolated throughout the world from healthy cattle, as well as from cattle exhibiting a variety of diseases. The biological life cycle of BoHV-4 relies, as for all Herpesviridae, on the existence of two types of infection: lytic (or replicative) and latent infections. The former leads to the production of progeny virions and generally to the lysis of the infected cell. During a lytic infection, the expression of herpesvirus proteins is temporally regulated. The proteins are classified into three kinetic classes depending on the order of their synthesis during the infection (49). These proteins are expressed chronologically as immediate-early (IE), early (E), and late (L) proteins. IE proteins are expressed directly after release of the viral genome from the capsid into the nucleus. Although E protein expression occurs after synthesis of IE proteins, L protein expression depends on the expression of both IE and E proteins and viral DNA synthesis. Latent infection consists of a dormant state associated with the expression of a limited number of viral genes which might affect the biology of the infected cell. Various stimuli can turn the latent infect...

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“…In vitro, BoHV-4 is able to replicate in primary cell cultures or cell lines from a broad spectrum of host species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, chickens, hamsters, rats, mice, and monkeys (12,(14)(15)(16)(17)(18)(19)(20)(21). Recently, several studies have shown that some human cell lines support BoHV-4 infection (15,(21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Bovine Herpesvirus 4 Induces Apoptosis of Human Carcinoma Cell Lines In vitro and In vivo

Gillet

Dewals

Farnir³

et al. 2005

Cancer Research

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The idea of using oncolytic viruses for the treatment of cancers was proposed a century ago. During the last two decades, viruses able to replicate specifically in cancer cells and to induce their lysis were identified and were genetically modified to improve their viro-oncolytic properties. More recently, a new approach consisting of inducing selective apoptosis in cancer cells through viral infection has been proposed; this approach has been called viro-oncoapoptosis. In the present study, we report the property of bovine herpesvirus-4 (BoHV-4) to induce, in vitro and in vivo, apoptosis of some human carcinomas. This conclusion relies on the following observations:

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Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus

Cited by 23 publications

References 43 publications

Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)

Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)

Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis

Bovine Herpesvirus 4 Induces Apoptosis of Human Carcinoma Cell Lines In vitro and In vivo

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