Establishment of a colonic adenoma cell line (GEKI-2): spectral karyotype analysis and functional characterization

Menzel, Thomas; Melcher, Ralph; Koehler, Sigrid; Dusel, Gerda; Backhaus, Kerstin; Ott, German; Breithaupt, Wolfram; Al-Taie, Oliver; Schauber, Jürgen; Gostner, Andrea; Scheppach, Wolfgang; Lührs, Hardi

doi:10.1007/s00384-003-0503-2

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…For example, CDK10 was found upregulated in tumor prostate specimens [ 49 ] and seminomas [ 50 ]. Conversely, CDK10 was found downregulated in retinoic acid-treated retinoblastoma cells [ 53 ] and in butyrate-treated colon carcinoma cells [ 57 ], where both treatments trigger cell cycle arrest. CDK10 transcripts were found to be downregulated in a multiple myeloma cell line treated with a histone deacetylase inhibitor that is currently being evaluated in clinical trials [ 48 ].…”

Section: Cdk10/cycm and Cancermentioning

confidence: 99%

The awakening of the CDK10/Cyclin M protein kinase

et al. 2017

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Cyclin-dependent kinases (CDKs) play important roles in the control of fundamental cellular processes. Some of the most characterized CDKs are considered to be pertinent therapeutic targets for cancers and other diseases, and first clinical successes have recently been obtained with CDK inhibitors. Although discovered in the pre-genomic era, CDK10 attracted little attention until it was identified as a major determinant of resistance to endocrine therapy for breast cancer. In some studies, CDK10 has been shown to promote cell proliferation whereas other studies have revealed a tumor suppressor function. The recent discovery of Cyclin M as a CDK10 activating partner has allowed the unveiling of a protein kinase activity against the ETS2 oncoprotein, whose degradation is activated by CDK10/Cyclin M-mediated phosphorylation. CDK10/Cyclin M has also been shown to repress ciliogenesis and to maintain actin network architecture, through the phoshorylation of the PKN2 protein kinase and the control of RhoA stability. These findings shed light on the molecular mechanisms underlying STAR syndrome, a severe human developmental genetic disorder caused by mutations in the Cyclin M coding gene. They also pave the way to a better understanding of the role of CDK10/Cyclin M in cancer.

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Section: Cdk10/cycm and Cancermentioning

confidence: 99%

The awakening of the CDK10/Cyclin M protein kinase

et al. 2017

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“…Nevertheless, incubation with butyrate resulted in a time-and dose-dependent inhibition of proliferation and in a significant increase in cellular differentiation. Thus colon adenoma cell line GEKI-2 may provide a versatile tool for further exploring the underlying mechanisms of protective and promoting factors in early colon cancerogenesis (Menzel et al, 2004).…”

Section: Chromosome Aberrations Detected In Adenocarcinomasmentioning

confidence: 99%

Spectral karyotyping of human, mouse, rat and ape chromosomes – applications for genetic diagnostics and research

Zschieschang

O’Brien

Helmrich

et al. 2006

Cytogenet Genome Res

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Spectral karyotyping (SKY) is a widely used methodology to identify genetic aberrations. Multicolor fluorescence in situ hybridization using chromosome painting probes in individual colors for all metaphase chromosomes at once is combined with a unique spectral measurement and analysis system to automatically classify normal and aberrant chromosomes. Based on countless studies and investigations in many laboratories worldwide, numerous new chromosome translocations and other aberrations have been identified in clinical and tumor cytogenetics. Thus, gene identification studies have been facilitated resulting in the dissection of tumor development and progression. For example, different translocation partners of the TEL/ETV6 transcription factor that is specially required for hematopoiesis within the bone marrow were identified. Also, the correct classification of complex karyotypes of solid tumors supports the prognostication of cancer patients. Important accomplishments for patients with genetic diseases, leukemias and lymphomas, mesenchymal tumors and solid cancers are summarized and exemplified. Furthermore, studies of disease mechanisms such as centromeric DNA breakage, DNA double strand break repair, telomere shortening and radiation-induced neoplastic transformation have been accompanied by SKY analyses. Besides the hybridization of human chromosomes, mouse karyotyping has also contributed to the comprehensive characterization of mouse models of human disease and for gene therapy studies.

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The butyrate story: old wine in new bottles?

Scheppach

Weiler

2004

Current Opinion in Clinical Nutrition and Metabolic Care

233

158

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In the era of molecular biology our understanding of subcellular processes that ultimately lead to inflammatory bowel disease or colorectal cancer has widened considerably. The new powerful technology of genomics and proteomics, however, raises new questions without easy answers. With this new information in mind, we will have to go back to human intervention trials to test the hypotheses generated in vitro. The preclinical data from the review period justify the need for carefully designed clinical trials to test the benefits derived from butyrate production.

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Establishment of a colonic adenoma cell line (GEKI-2): spectral karyotype analysis and functional characterization

Abstract: The presented colon adenoma cell line GEKI-2 may prove a versatile tool for further exploring the underlying mechanisms of protective and promoting factors in early colon cancerogenesis.

Cited by 4 publications

References 16 publications

The awakening of the CDK10/Cyclin M protein kinase

The awakening of the CDK10/Cyclin M protein kinase

Spectral karyotyping of human, mouse, rat and ape chromosomes – applications for genetic diagnostics and research

The butyrate story: old wine in new bottles?

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