Establishment of A Competitive ELISA (cELISA) System for the Detection of Influenza A Virus Nucleoprotein Antibodies and its Application to Field Sera from Different Species

Starick, Elke; Werner, Ortrud; Schirrmeier, Horst; Köllner, Bernd; Riebe, Roland; Mundt, Egbert

doi:10.1111/j.1439-0450.2006.01007.x

Cited by 57 publications

(46 citation statements)

References 15 publications

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“…All sera were tested at DAFWA with a NP competitive enzyme-linked immunosorbent assay (NP c-ELISA) targeting influenza group A-specific NP antibodies using reagents supplied by the Australian Animal Health Laboratory (AAHL) and based on standard methods (18). At DAFWA, optical densities were read at 450 nm (Multiskan EX Microplate Photometer, Thermo Labsystems, China), with test serum results calculated as the percentage inhibition of binding of the monoclonal antibody in the absence of any serum.…”

Section: Methodsmentioning

confidence: 99%

Variation in the Responses of Wild Species of Duck, Gull, and Wader to Inoculation with a Wild-Bird–Origin H6N2 Low Pathogenicity Avian Influenza Virus

Curran

Robertson

Ellis³

et al. 2013

Avian Diseases

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SUMMARY. There is poor understanding of host responses to avian influenza virus (AIV) infection in wild birds, with most experimental studies using captive-bred birds and highly pathogenic AIVs that have an early endpoint. The objective of this study was to experimentally assess antibody responses and patterns of viral excretion in wild birds challenged with a low pathogenicity AIV. Ruddy turnstones (Arenaria interpres), silver gulls (Chroicocephalus novaehollandiae), and wandering whistling ducks (Dendrocygna arcuata) were challenged with a H6N2 virus, and blood, cloacal, and oropharyngeal (OP) swabs were analyzed from each bird over 28 days, with serology conducted on the ducks for a further 7 mo. Nineteen of 22 birds showed evidence of infection, with respiratory infection prevalent in the turnstones and gulls as mostly low titer viral excretion to 4 days postinoculation (DPI) with gastrointestinal replication detected in only one turnstone. In AIV naive ducks, there was gastrointestinal tropism with moderately high titer viral excretion via the cloaca to 6 DPI and low-grade OP viral excretion to 4 DPI. The hemagglutination inhibition antibody response was poor in the ducks, declining from 19 to 56 DPI, with higher titer responses in the gulls and turnstones. All infected birds responded with elevated nucleoprotein antibodies (in competitive enzymelinked immunosorbent assay) by 7-10 DPI, and in the ducks these waned slowly after 42 DPI and were long-lived to at least 8 mo. The interspecies variability in response was consistent with a subtype that had adapted well in ducks, while the response of the turnstones may have been influenced by preexisting immunity to AIV. These findings provide insight into AIV infection dynamics in wild birds and highlight the need for further research.RESUMEN. Variación en las respuestas de las especies silvestres de pato, gaviota y aves limícolas a la inoculación con un virus de influenza aviar de baja patogenicidad de origen aviar subtipo H6N2.Existe poca información sobre las respuestas del huésped a la infección por el virus de la influenza aviar (AIV) en aves silvestres, con la mayoría de estudios experimentales realizados con aves criadas en cautividad y los virus de influenza aviar de alta patogenicidad que tienen un punto final temprano. El objetivo de este estudio fue evaluar experimentalmente las respuestas de anticuerpos y los patrones de excreción viral en las aves silvestres expuestas a un virus de influenza aviar de baja patogenicidad. Vuelvepiedras comunes (Arenaria interpres), gaviotas plateadas (Larus novaehollandiae) y yaguasas errantes (Dendrocygna arcuata) fueron expuestas a un virus H6N2, y muestras de sangre, hisopos cloacales y orofaríngeos se analizaron de cada ave durante 28 días, la serología se llevó a cabo en los patos por siete meses. Diecinueve de 22 aves mostraron evidencia de infección, con infección respiratoria frecuente en los vuelvepiedras y en las gaviotas principalmente con excreción viral con título bajo a los cuatro días después de la inocu...

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Section: Methodsmentioning

confidence: 99%

Variation in the Responses of Wild Species of Duck, Gull, and Wader to Inoculation with a Wild-Bird–Origin H6N2 Low Pathogenicity Avian Influenza Virus

Curran

Robertson

Ellis³

et al. 2013

Avian Diseases

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“…Competitive ELISAs are widely used for serological detection of antibodies to influenza viruses, mainly due to their sensitivity and simplicity (25,27,30). Their significant advantage is their species-nonspecific approach.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Diagnostic Applications of Monoclonal Antibodies against Avian Influenza H7 Viruses

Yang

Clavijo

Graham

et al. 2010

Clin Vaccine Immunol

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“…Serum samples were screened using an Australian Animal Health Laboratory (AAHL) nucleoprotein competitive enzymelinked immunosorbent assay (NP c-ELISA) for AIV antibody at either AAHL or at the Department of Food and Agriculture WA (DAFWA), based on standard methods (19). Most c-ELISA positive samples (.60% inhibition) and many in the weak positive range (40%-60% inhibition) were also tested by hemagglutination inhibition (HI) (22), using subtype H5 and H7 antigens, and where possible against a panel of H1-H16 antigens, subject to availability of sera and antigens.…”

Section: Methodsmentioning

confidence: 99%

Serological Surveillance of Wild Waterfowl in Northern Australia for Avian Influenza Virus Shows Variations in Prevalence and a Cyclical Periodicity of Infection

2015

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SUMMARY. The virological surveillance of 3582 wild waterfowl in northern Australia from 2004 to 2009 for avian influenza virus (AIV) found an apparent prevalence (AP) of 1% (31 of 2989 cloacal swabs; 95% CI: 0.71%-1.47%) using a Taqman Type A real-time reverse transcription polymerase chain reaction test and no viral isolations from 593 swabs tested by the embryonating chicken egg culture method. From serological testing using a nucleoprotein competitive enzyme-linked immunosorbent assay for AIV antibody, 1131 of 3645 sera had $40% inhibition, indicating an apparent seroprevalence of 31% (95% CI: 29.5%-32.6%). This value suggests that the low AP from virological testing does not reflect the dynamics of AIV infection in these populations. Spatiotemporal and species variations in seroprevalence were found at wetland sampling sites, with consistently higher values at Kununurra in Western Australia (AP 5 39%, 95% CI: 36.9%-41.4%) compared to other locations. At Kununurra, seroprevalence values had a two-year cyclical periodicity and suggest this location is a hotspot of AIV activity. From hemagglutination inhibition (HI) testing using multiple subtype antigens, the highest AP of HI reactions were to H6 and H5 subtypes. The phenomenon of cyclic periodicity in NP seroprevalence at Kununurra is hypothesized as being related to the prevalent H6 subtype that may have either become predominant or cycled back into a mostly AIV naïve flock. The inclusion of serological testing provided insight into the dynamics of AIV infection in wild birds such as species risk profiles and spatiotemporal patterns, important epidemiological information for a risk-based approach to surveillance.RESUMEN. La vigilancia serológica de aves acuáticas silvestres en el norte de Australia para el virus de la influenza aviar muestra variaciones en la prevalencia y en la periodicidad cíclica de la infección.La vigilancia virológica de 3,582 aves acuáticas silvestres en el norte de Australia del año 2004 al 2009 para el virus de la influenza aviar (AIV) mostró una prevalencia aparente (AP) de 1% (31 de un total de 2,989 hisopos cloacales; con un intervalo de confianza 95%: 0.71% -1.47%) utilizando un método de transcripción reversa y reacción en cadena de la polimerasa con un Taqman tipo A sin aislamiento viral de 593 hisopos analizados por el método de cultivo en huevos embrionbados de pollo. A partir de las pruebas serológicas utilizando un ensayo inmunoenzimático competitivo para anticuerpos contra la nucleoproteína del virus de la influenza aviar, 1,131 de 3,645 sueros mostraron $40% de inhibición, lo que indica una aparente seroprevalencia del 31% (IC del 95%: 29.5% -32.6%). Este valor indica que la baja prevalencia aparente mostrada por las pruebas virológicas no refleja la dinámica de la infección por el virus de la influenza aviar en estas poblaciones. Variaciones espacio-temporales y por la especie en la seroprevalencia fueron encontradas en sitios de muestreo localizados en humedales, con valores consistentemente altos en Kununurra en Austra...

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Establishment of A Competitive ELISA (cELISA) System for the Detection of Influenza A Virus Nucleoprotein Antibodies and its Application to Field Sera from Different Species

Cited by 57 publications

References 15 publications

Variation in the Responses of Wild Species of Duck, Gull, and Wader to Inoculation with a Wild-Bird–Origin H6N2 Low Pathogenicity Avian Influenza Virus

Variation in the Responses of Wild Species of Duck, Gull, and Wader to Inoculation with a Wild-Bird–Origin H6N2 Low Pathogenicity Avian Influenza Virus

Evaluation of Diagnostic Applications of Monoclonal Antibodies against Avian Influenza H7 Viruses

Serological Surveillance of Wild Waterfowl in Northern Australia for Avian Influenza Virus Shows Variations in Prevalence and a Cyclical Periodicity of Infection

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