Evaluation of Diagnostic Applications of Monoclonal Antibodies against Avian Influenza H7 Viruses

Yang, Ming; Clavijo, Alfonso; Graham, Jill; Pasick, John; Neufeld, James; Berhane, Yohannes

doi:10.1128/cvi.00023-10

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…High and Low pathogenic viruses were propagated in 11-day old embryonated chicken eggs and virus titers were determined by hemagglutinin assay [13] . Viruses were clarified by centrifugation at 13000×g for 15 min and inactivated using binary ethylenimine (BEI) to a final concentration of 0.01 M for 24 h at 37°C [14] and stored at −80°C. All experiments with live viruses were performed in a biosafety level 3 containment laboratory in compliance with CDC/NIH and WHO recommendations [15] and also approved by the Agri-food and Veterinary agency and Ministry of Health of Singapore.…”

Section: Methodsmentioning

confidence: 99%

A Novel Peptide ELISA for Universal Detection of Antibodies to Human H5N1 Influenza Viruses

Velumani

et al. 2011

PLoS ONE

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BackgroundActive serologic surveillance of H5N1 highly pathogenic avian influenza (HPAI) virus in humans and poultry is critical to control this disease. However, the need for a robust, sensitive and specific serologic test for the rapid detection of antibodies to H5N1 viruses has not been met.Methodology/Principal FindingsPreviously, we reported a universal epitope (CNTKCQTP) in H5 hemagglutinin (HA) that is 100% conserved in H5N1 human isolates and 96.9% in avian isolates. Here, we describe a peptide ELISA to detect antibodies to H5N1 virus by using synthetic peptide that comprises the amino acid sequence of this highly conserved and antigenic epitope as the capture antigen. The sensitivity and specificity of the peptide ELISA were evaluated using experimental chicken antisera to H5N1 viruses from divergent clades and other subtype influenza viruses, as well as human serum samples from patients infected with H5N1 or seasonal influenza viruses. The peptide ELISA results were compared with hemagglutinin inhibition (HI), and immunofluorescence assay and immunodot blot that utilize recombinant HA1 as the capture antigen. The peptide ELISA detected antibodies to H5N1 in immunized animals or convalescent human sera whereas some degree of cross-reactivity was observed in HI, immunofluorescence assay and immunodot blot. Antibodies to other influenza subtypes tested negative in the peptide-ELISA.Conclusion/SignificanceThe peptide-ELISA based on the highly conserved and antigenic H5 epitope (CNTKCQTP) provides sensitive and highly specific detection of antibodies to H5N1 influenza viruses. This study highlighted the use of synthetic peptide as a capture antigen in rapid detection of antibodies to H5N1 in human and animal sera that is robust, simple and cost effective and is particularly beneficial for developing countries and rural areas.

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Section: Methodsmentioning

confidence: 99%

A Novel Peptide ELISA for Universal Detection of Antibodies to Human H5N1 Influenza Viruses

Velumani

et al. 2011

PLoS ONE

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“…Compared with polyclonal antibodies, the mAb-based immunoassays owned the advantages of stability and specificity, and they may be exploited for diagnostic applications and other research. Although several studies on mAbs against avian influenza H7N9 HA have been reported (Yang et al, 2010;Malik et al, 2016;Schmeisser et al, 2015 a few focused on the neutralization effect (Malik et al, 2016, Schmeisser et al, 2015, Stadlbauer et al, 2018, and most of the diagnostic applications of the mAbs were derived from avian H7 protein prior to the novel influenza A (H7N9) virus outbreak (Vasudevan et al, 2018;Yang et al, 2010). In this study, the HA derived from novel H7 subtype influenza (A/Anhui/1/2013, H7N9) was used as immunogen for the mAb development.…”

Section: Discussionmentioning

confidence: 99%

Development and optimized pairing of mouse monoclonal antibodies for detecting hemagglutinin in novel H7 subtype influenza viruses

Wang

Zhou

Wang

et al. 2019

Sci. China Life Sci.

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The H7 subtype avian influenza threatens public health with respect to poultry and humans. Thus, a specific and sensitive diagnostic test is essential for the management of H7 subtype influenza infections. In this study, five mouse monoclonal antibodies (mAbs) against hemagglutinin (HA) of influenza A/Anhui/1/2013 (H7N9) were produced and characterized by the Western blot, immunofluorescence, and hemagglutination inhibition assays. All five specific mAbs reacted with the HA protein of H7N9 but not with that of H1N1, H3N2, or H5N1. With the combination arrays of capture and detection antibodies, the matched pair mAbs (1C4-coated and 2D7-labeled) were selected and employed in a double-antibody sandwich ELISA (DAS-ELISA). Detection limits of the sandwich ELISA were 0.45 ng mL -1 for the HA protein derived from A/Anhui/1/2013 (H7N9); or 1 and 2 HA units/50 µL for A/Anhui/1/2013 (H7N9) and A/GD/17SF003/2016 (H7N9), respectively. These anti-HA mAbs against subtype H7 and the novel DAS-ELISA provide a valuable approach for specific detection of the H7 subtype influenza virus and quantification of its HA protein, especially for the novel epidemic H7N9.influenza A, H7 subtype, hemagglutinin, monoclonal antibodies, sandwich ELISA Tan, W. (2020). Development and optimized pairing of mouse monoclonal antibodies for detecting hemagglutinin in novel H7 subtype influenza viruses. Sci China Life Sci 63, 279-289. https://doi.

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“…For a decade, many studies have sought to develop H7 subtype-specific rapid diagnostic immunoassays. Three ELISA-based H7 subtype- specific systems have been developed 27 – 29 . Recently, H7 subtype-specific Abs were reported to have vaccine efficacy 30 , 31 .…”

Section: Discussionmentioning

confidence: 99%

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

Yeo

Bao

Seo

et al. 2017

Sci Rep

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The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.

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Evaluation of Diagnostic Applications of Monoclonal Antibodies against Avian Influenza H7 Viruses

Cited by 8 publications

References 38 publications

A Novel Peptide ELISA for Universal Detection of Antibodies to Human H5N1 Influenza Viruses

A Novel Peptide ELISA for Universal Detection of Antibodies to Human H5N1 Influenza Viruses

Development and optimized pairing of mouse monoclonal antibodies for detecting hemagglutinin in novel H7 subtype influenza viruses

Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

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