Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma

Abstract

BackgroundEndonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.ResultsThe method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P < 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P = 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P = 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P = 0.736 > 0.05 and P = 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P = 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P = 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).ConclusionsAn assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.

MUS81 Participates in the Progression of Serous Ovarian Cancer Associated With Dysfunctional DNA Repair System

“…But after UV treatment, the results were dramatically changed, the percentage of G0/G1 in MUS-KD cells was significantly decreased, and cell cycle progression was arrested at S phase (Figure 2CII). In our related study, we also found that the total endonuclease activity of MUS-KD cells was decreased due to the arrest of DNA synthesis in mitosis after MUS81 silencing (18).…”