Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus

Miyazawa, Takayuki; Furuya, Tetsuya; Itagaki, Shin-ichi; Tohya, Yukinobu; Takahashi, Eiji; Mikami, Takeshi

doi:10.1007/bf01313750

Cited by 136 publications

(78 citation statements)

References 8 publications

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“…The Petaluma isolate grew well even in CD4-and CD8-negative CrFK cells, and cells persistently infected with this isolate were also established (Yamamoto et al, 1988). However, the Japanese isolates classified as subtype B, FIV/TM-1 and TM-2, did not grow in CrFK cells (Miyazawa et al, 1989). It has also been reported that some viruses isolated in lymphoblastoid cells could be adapted to grow in CrFK cells only after several passages in the lymphoblastoid cell line (Baldinotti et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Hohdatsu

Hirabayashi

Motokawa

et al. 1996

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Hohdatsu

Hirabayashi

Motokawa

et al. 1996

Journal of General Virology

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“…Antigens were prepared from viral cultures of domestic cat FIV (FIV-B2546), puma lentivirus (PLV-1695), and lion lentivirus (LLV-438). Stocks were grown in domestic cat origin cell line Mya-1 (Miyazawa et al, 1989), and viral proteins were isolated as previously described (VandeWoude et al, 1997b;TerWee et al, 2005). Reverse-transcriptase positive tissue culture supernatant was centrifuged at 200 3 G (GPR centrifuge, Beckman Instruments, Inc., Fullerton, California, USA) for 10 min at 5 C to remove cellular material.…”

Section: Immunoblot Analysismentioning

confidence: 99%

Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)

Franklin

Troyer

TerWee

et al. 2007

Journal of Wildlife Diseases

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ABSTRACT:Although lentiviruses similar to feline immunodeficiency virus (FIV) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. We tested bobcats (Lynx rufus), pumas (Felis concolor), and ocelots (Leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (PLV), African lion lentivirus (LLV), and domestic cat FIV-based immunoblots, a commercially available enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR). Puma lentivirus immunoblots identified more seropositive individuals than the other antibody-detection assays. The commercial ELISA provided a fair ability to recognize seropositive samples when compared with PLV immunoblot for screening bobcats and ocelots, but not pumas. Polymerase chain reaction identified fewer positive samples than PLV immunoblot for all three species. Immunoblot results were equivalent whether the sample tested was serum, plasma, or whole blood. The results from this study and previous investigations suggest that the PLV immunoblot has the greatest ability to detect reactive samples when screening wild felids of North America and is unlikely to produce false positive results. However, the commercial ELISA kit may provide an adequate alternative for screening of some species and is more easily adapted to field conditions.

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“…To allow accurate estimation of the proviral load per 10 6 mononuclear cells, a further Taqman PCR designed to measure total sample DNA was performed using primers and probe speci®c for the rRNA gene (Klein et al, 2000). Standards for this PCR were derived from MYA-1 (Miyazawa et al, 1989) genomic DNA. The DNA was quanti®ed by measuring the absorbance at OD 260 and a dilution series was prepared in PCR grade water as before.…”

Section: Determination Of Fiv Proviral Loadmentioning

confidence: 99%

Involvement of cytolytic and non-cytolytic T cells in the control of feline immunodeficiency virus infection

Flynn

Dunham

Mueller

et al. 2002

Veterinary Immunology and Immunopathology

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The appearance of non-cytolytic T cells that suppressed feline immunode®ciency virus (FIV) replication in vitro, and FIV-speci®c cytotoxic T cell (CTL) responses was compared in a group of seven, speci®c pathogen free (SPF) domestic cats following primary infection with the Glasgow 8 isolate of FIV (FIV ). FIV proviral burdens were quanti®ed in the blood and lymphoid tissues by real-time PCR. Non-cytolytic T cell suppression of FIV replication was measured by co-cultivating lymphoblasts prepared from the cats at different time-points during infection with FIV-infected MYA-1 cells in vitro. Non-cytolytic suppressor activity was detected as early as 1 week after infection, and was evident in all the lymphoid tissues examined. Further, this activity was present in subpopulations of T cells in the blood with normal (CD8 hi ) or reduced (CD8 lo ) expression of the CD8 molecule, and temporal modulations in non-cytolytic suppressor activity were unrelated to the circulating CD8 T cell numbers. Virus-speci®c CTL responses, measured by 51 Cr release assays, were not detected until 4 weeks after infection, with the emergence of FIV-speci®c effector CTLs in the blood. Throughout infection the response was predominantly directed towards FIV Gag-expressing target cells, and by 47 weeks after infection CTL responses had become localised in the lymph nodes and spleen. The results suggest that both non-cytolytic T cell suppression of FIV replication and FIV-speci®c CTL responses are important cellular immune mechanisms in the control of FIV replication in infected asymptomatic cats. #

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Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus

Cited by 136 publications

References 8 publications

Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Comparative study of the cell tropism of feline immunodeficiency virus isolates of subtypes A, B and D classified on the basis of the env gene V3-V5 sequence

Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)

Involvement of cytolytic and non-cytolytic T cells in the control of feline immunodeficiency virus infection

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