Establishment of a Human Medulloblastoma Cell Line and Its Heterotransplantation into Nude Mice

Inhibition of medulloblastoma cell invasion by Slit

“…Confluent cultures of U251 (human glioblastoma) UW228-3 (UW3) (human medulloblastoma cell line derived from one of three cell aliquots taken from the same tumor resected from a 9-year-old girl) (Keles et al, 1995), and Daoy (human medulloblastoma derived from tumor biopsy from a 4-yearold boy) (Jacobson et al, 1985), HEK 293 and HEK 293 cells stably overexpressing full-length human Slit2 with a c-Myc tag at the carboxy terminus were trypsinized (0.05% trypsin/ 0.53 mM ethylenediamine-N, N, N 0 , N 0 -tetraacetic acid) and hanging drops were prepared (Corcoran and Del Maestro, 2003;Del Duca et al, 2004). Aggregates consisting of 40 000 cells/drop and 25 000 cells/drop (U251) were utilized.…”

Metabolic studies of human primitive neuroectodermal tumour cells by proton nuclear magnetic resonance spectroscopy

“…MHH-MED-3 and MHH-MED-4 were obtained from biopsies of cerebellar medulloblastomas, while MHH-MED-1 was generated from cells recovered from the cerebrospinal fluid of a patient with a recurrent cerebellar PNET that had seeded the cerebrospinal fluid. D283-MED and DAOY were lines previously established by Friedman et al (1985) and Jacobsen et al (1985). Other cell lines (MHH-PNET-5 and MHH-PNET-6) were obtained from PNETs of other locations in the CNS.…”

“…Questions have been raised as to whether their cell of origin is unique to the portion of the CNS in which the tumour arises, or whether there is a primitive or undifferentiated cell common to all portions of the CNS. Rorke et al (1985) have suggested that these tumours arise from a single primitive multipotential cell that has the capacity to differentiate into one or more types of neural cellssuch as astrocytes, oligodendrocytes, neurons, ganglion cells or melanocytes -and are therefore regarded as malignant counterparts of multipotential neural progenitor cells.Progress in understanding the cell biology of these tumours has been hampered by the lack of cultured cell lines, since relatively few continuous medulloblastoma lines have been established thus far (Friedman et al, 1985;Jacobsen et al, 1985). However, the recent establishment of five new human PNET cell lines from surgical specimens (Pietsch et al, 1994) Correspondence to: S Williams investigate the biology, phenotype, lineage and metabolism of PNETs in detail.…”

“…Progress in understanding the cell biology of these tumours has been hampered by the lack of cultured cell lines, since relatively few continuous medulloblastoma lines have been established thus far (Friedman et al, 1985;Jacobsen et al, 1985). However, the recent establishment of five new human PNET cell lines from surgical specimens (Pietsch et al, 1994) Correspondence to: S Williams investigate the biology, phenotype, lineage and metabolism of PNETs in detail.…”

Survivin, Survivin-2B, and Survivin-deItaEx3 expression in medulloblastoma: biologic markers of tumour morphology and clinical outcome

“…Daoy cells (ATCC #HTB-186) are human medulloblastoma cells originally established from a medulloblastoma tumour isolated from the posterior fossa of a 4 year-old child (Jacobsen et al, 1985). Cells were grown in Dulbecco's modified essential medium supplemented with 10% fetal bovine serum and 2 mM L-glutamine.…”