Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis

Helbig, Lars; Simank, H.-G.; Lorenz, Helga; Putz, Cornelia; Wölfl, Christoph; Suda, Arnold J.; Moghaddam, Arash; Schmidmaier, Gerhard; Guehring, Thorsten

doi:10.1007/s00264-013-2149-1

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…ese results showed that these parameters could be useful for monitoring osteomyelitis. In the development of an MRSA animal model of osteomyelitis, Helbig et al [54] monitored infection by measuring body temperature and body weight in combination with other parameters.…”

Section: Discussionmentioning

confidence: 99%

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

Hriouech

Akhmouch

Mzabi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The aim of this research paper is to test the antistaphylococcal effect of 1,8-cineole, amoxicillin/clavulanic acid (AMC), and gentamicin, either separately or in combination against three Staphylococcus aureus strains isolated from patients suffering from osteomyelitis. This activity was tested in vitro by using the microdilution method and the checkerboard assay. The efficacy of these three antibacterial agents was then tested in vivo by using an experimental model of methicillin-resistant S. aureus osteomyelitis in rabbits. This efficacy was assessed after four days of treatment by counting the number of bacteria in the bone marrow. The obtained results in vitro showed that the combination of the AMC with gentamicin did not induce a synergistic effect, whereas the combination of the two antibiotics with 1,8-cineole did. This effect is stronger when AMC is combined with 1,8-cineole as a total synergistic effect was obtained on the three strains used (FIC ≤ 0.5). In vivo, a significant reduction was noted in the number of colonies in the bone marrow when rabbits were treated with AMC associated with either 1,8-cineole or gentamicin compared to rabbits treated with AMC, gentamicin, or 1,8-cineole alone. These results demonstrated that 1,8-cineole showed a synergistic effect in combination with both AMC and gentamicin, which offer possibilities for reducing antibiotic usage. Also, the AMC associated with 1,8-cineole could be used to treat MRSA osteomyelitis.

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Section: Discussionmentioning

confidence: 99%

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

Hriouech

Akhmouch

Mzabi

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In vitro previous studies showed that modification of bone spacer cements with microspheres of PLGA or any other formulations improved the release of antibiotics to prevent infection and the ability to remodel a bone defect . These steps are the basis for future improvements in commercial bone spacer formulations, which will reduce the morbidity associated with these interventions in a medium term.…”

Section: Discussionmentioning

confidence: 99%

Polylactic‐co‐glycolic acid microspheres added to fixative cements and its role on bone infected architecture

et al. 2019

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Joint prostheses are an essential element to improve quality of life. However, prostheses may fail due to several factors, including the most frequent cause, Staphylococcus aureus infection. The identification of new fixing bone cements with less reactivity on bone tissue and an adequate response to infection remains a primary challenge. The aim of this study is to evaluate the response of bone tissue in rabbits after introduction of a hydroxyapatite‐coated titanium rod with a commercial fixative cement (Palacos®) compared to a modified experimental cement (EC) containing polylactic‐co‐glycolic acid (PLGA) microspheres in the presence or absence of contaminating germs. This study used 20 New Zealand rabbits which were divided into four groups (n = 5) depending on the presence or absence of S. aureus and the use of commercial (Palacos®) or EC. A histological method, based on bone architecture damage, was proposed to evaluate from 1 to 9 the histological results and the response of the infected tissue. The macrophage response was also evaluated using monoclonal antibody RAM‐11. The study showed better bone conservation with the use of EC with PLGA microspheres against the Palacos® commercial cement, including the noncontaminated and contaminated groups. © 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2517–2526, 2019.

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“…Although growth characteristics of several animal models can be compared with reasonable approximation to human reality, the ability of the induced membrane to assist in the healing of critical sized bone defect is still under investigation. However, animal models appear to be valuable for future experimental use and outcome assessment of new treatment protocols [4,[17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Chronic infection and infected non-union of the long bones in paediatric patients: preliminary results of bone versus beta-tricalcium phosphate grafting after induced membrane formation

Rousset

Walle

Cambou

et al. 2017

International Orthopaedics (SICOT)

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Establishment of a new methicillin resistant staphylococcus aureus animal model of osteomyelitis

Cited by 16 publications

References 30 publications

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

The Antistaphylococcal Activity of Amoxicillin/Clavulanic Acid, Gentamicin, and 1,8‐Cineole Alone or in Combination and Their Efficacy through a Rabbit Model of Methicillin‐Resistant Staphylococcus aureus Osteomyelitis

Polylactic‐co‐glycolic acid microspheres added to fixative cements and its role on bone infected architecture

Chronic infection and infected non-union of the long bones in paediatric patients: preliminary results of bone versus beta-tricalcium phosphate grafting after induced membrane formation

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