Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach

Abstract

Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research.

Benzo[a]pyrene promotes progression in tongue squamous cell carcinoma

“…At concentrations representative of human exposure, high dose of B[a]P (up to 1 mM) toxicity on cancer progression has been reported, (Dong et al, 2015;Lee, Yang, Ho, Tsai, & Chang, 2008).…”

Prediction of biomarkers of oral squamous cell carcinoma using microarray technology

“…About half were oral squamous cell carcinoma (OSCC) 2 , which was a highly aggressive head and neck tumor and prone to local recurrence and metastasis 3 . The development of OSCC was a long-time, multi-stage and multi-factor process, and many regulatory factors were involved in cell carcinogenesis 4 . However, the detailed molecular mechanism of this cancer was still unclear.…”

Study of the TP53 codon 72 polymorphism in oral cancer and oral potentially malignant disorders in Argentine patients

“…The importance of OL lies in its propensity for malignant transformation. 29 The use of molecular biology techniques to diagnose oral precancerous lesions and cancer may markedly improve the early detection of alterations that are invisible under the microscope. 30 The presence and the accumulation of genetic changes have been shown to be associated with the risk of malignant transformation.…”