Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome

Abstract

A megakaryoblastic cell line, designated MEG-01, was established from the bone marrow of a patient with blast crisis of Philadelphia (Ph1) chromosome-positive chronic myelogenous leukemia. MEG-01 cells grew in single-cell suspension with a doubling time of 36 to 48 hours. Under the usual culture conditions, approximately half of the cells adhered to the culture flask with extention of pseudopods. MEG-01 cells were positive for the periodic acid-Schiff reaction, alpha-naphthyl acetate esterase, and acid phosphatase, and negative for myeloperoxidase, alpha- naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase. Ultrastructural platelet peroxidase was positive in MEG-01 cells. Cytoplasmic factor VIII (FVIII)-related antigen was weakly positive in larger MEG-01 cells by both an indirect immunofluorescent technique with monoclonal antibodies and a direct immunoperoxidase technique using horseradish peroxidase-conjugated conventional rabbit anti-human FVIII antibody. Platelet glycoprotein (GP) IIb/IIIa antigen was uniformly demonstrated on the surface of MEG- 01 cells by both indirect immunofluorescent and immunoperoxidase techniques using antiplatelet GP IIb/IIIa monoclonal antibodies; platelet GP lb antigen was demonstrated only in the cytoplasm of larger MEG-01 cells. MEG-01 cells possessed no markers for B or T lymphocytes or for myeloid cells. Chromosome analysis of this cell line revealed a human male hyperdiploid karyotype with a modal chromosome number of 56 to 58. The Ph1 chromosome was observed in all karyotypes analyzed. This novel human megakaryoblastic cell line may provide a useful model for the study of human megakaryopoiesis and of the biosynthetic mechanisms of proteins unique to megakaryocytic lineage.

Proplatelet formation in megakaryocytes is associated with endoplasmic reticulum stress

“…In this study, we analyzed caspase activation during maturation of a human megakaryoblastic cell line, MEG‐01 (Ogura et al . ). The MEG‐01 cell line (referred to as megakaryocytes in this study) matures spontaneously to generate proplatelets that undergo fragmentation into platelet‐like particles (PLPs), albeit very rarely (Clarke et al .…”

Effects of inhibitor of Src kinases, SU6656, on differentiation of megakaryocytic progenitors and activity of alpha1,6-fucosyltransferase.

“…The Meg-01 cell line (Ogura et al, 1985) derived from a Ph + chronic myelogenous leukaemia (CML) patient in blast crisis was purchased from the American Type Culture Collection (ATCC). Meg-01 cells were cultured at 5 × 10 5 /mL in RPMI 1640 (Gibco) supplemented with 10% FBS (Gibco) and antibiotic antimycotic solution (penicillin G, streptomycin sulphate, amphotericin B; Sigma).…”

“…The activity of FUT8 in Meg-01 is 4-fold higher than in the lymphoblastic cell line K562 (morphology -lymphoblast; CML Ph + ; FUT8 = 25.7 ± 2.8 fmol/h per 10 3 cells) or Jurkat cells (morphology -lymphoblast; lymphoma; FUT8 = 27.2 ± 3.0 fmol/h per 10 3 cells), which confirms the special status of the enzyme in Meg-01 (not shown). Meg-01 cells are megakaryoblasts in a relatively early stage (Ogura et al, 1985). The presence of SU6656 in culture medium clearly induced differentiation and maturation of Meg-01 cells.…”

Erythropoiesis and Megakaryopoiesis in a Dish

“…Multiple cell lines have been generated to study megakaryopoiesis with among them; MEG-01 (suspension cells) and DAMI (adherent/suspension cells) both megakaryoblastic leukemia cell lines [108,109]. These cell lines are mostly positive for MK-specific markers (see in Section 2.3.3) but are not a homogenous population.…”