Establishment of a serodiagnosis system for the detection of Toxocara spp. and Ascaris suum infection in chickens

Nguyen, Yen Thi Hoang; Hayata, Yayoi; Sonoda, Shino; Nonaka, Nariaki; Maruyama, Haruhiko; Yoshida, Ayako

doi:10.1016/j.parint.2019.102022

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…In addition, false-positive results in serological assays may occur in coinfections with other helminths due to cross-reactivity, as all Ascarid parasites may share a high homology of antigenicity with Toxocara spp. ( 68 ). Reduction of cross-reactivity in the Toxocara ELISA test has been obtained by preincubation with extract of adult Ascaris suum , removing antibodies elicited by exposure to Ascaris ( 10 , 69 ).…”

Section: Discussionmentioning

confidence: 99%

Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis

Merigueti

Giuffrida

Silva

et al. 2022

Front. Public Health

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Toxocariasis, a neglected parasitic zoonosis with worldwide distribution, has been reportedly associated to different risk factors in several epidemiological and meta-analysis studies. However, dog and cat contact (environmental and animal exposure) as isolated associated risk factor for children and adults remains to be fully established. Accordingly, the present meta-analysis has aimed to directly assess dog and cat contact for toxocariasis seropositivity in under-18 and adult persons, using a survey strategy of PubMed/Medline, Embase, Scopus and Scielo Databases, from January 2009 to December 2021. A meta-analysis model of random effects was applied to estimate odds ratio (OR) with 95% Confidence Interval (CI). The statistical heterogeneity was evaluated by the Cochran Q-Test and I2 values. A total of 41 transversal studies (n = 20.515 individuals) from different geographic regions (classified by the World Health Organization) were included herein. In overall, 1,882/13,496 (13.95%; 95% IC = 13.4–14.5) youngers and 513/7.019 (7.3%; 95% CI = 6.7–7.9) adults in contact with dogs or cats were serologically reagent for anti-Toxocara antibodies. Association of dog and cat contact was observed only in youngers, with both dogs (OR = 1.53; p < 0.0001) and cats (OR = 1.64; p = 0.0001). In addition, association of dog and contact and serology was statistically significant in populations of Americas (OR = 1.37; 95% CI = 1.1–1.7), Middle East (OR = 2.9; 95% CI = 1.6–5.1) and West Pacific (OR = 1.6; 95% IC = 1.3–1.9). In conclusion, contact with dogs and cats, particularly by younger individuals and in regions such as Americas, Middle East, and West Pacific, should be always a public health concern for toxocariasis. Moreover, dogs and cats should be periodically dewormed, washed and hair cleaned prior to contact with youngers. Finally, robust statistical results herein may serve as basis for future strategies and preventive measures for safer dog and cat contact.

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Section: Discussionmentioning

confidence: 99%

Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis

Merigueti

Giuffrida

Silva

et al. 2022

Front. Public Health

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“…Recently, an ELISA system to distinguish A. suum to Toxocara spp. infection was successfully developed for diagnosis in chickens [ 10 ]. However, this method cannot always identify the presence of larval contamination in meat/offal because serological tests reflect not only present infections, but also past ones.…”

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confidence: 99%

Application of a real-time PCR assay for the detection of <i>Ascaris suum</i> DNA in the liver of experimentally infected chickens

Nguyen

Nonaka

Maruyama

et al. 2021

The Journal of Veterinary Medical Science

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This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.

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Establishment of a serodiagnosis system for the detection of Toxocara spp. and Ascaris suum infection in chickens

Cited by 2 publications

References 33 publications

Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis

Dog and Cat Contact as Risk Factor for Human Toxocariasis: Systematic Review and Meta-Analysis

Application of a real-time PCR assay for the detection of <i>Ascaris suum</i> DNA in the liver of experimentally infected chickens

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