Establishment of a simple reproducible model for antibiotic sensitivity pattern study of biofilm forming staphylococcus aureus

Baishya, Rimashree; Bhattacharya, Anusuya; Mukherjee, Madhurima; Lahiri, Dibyajit; Banerjee, Soma

doi:10.1016/j.matpr.2016.10.028

Cited by 13 publications

(8 citation statements)

References 14 publications

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“…The mode of action of amikacin, an aminoglycoside, is diffusing through the outer membrane of the bacteria and binding the 30S ribosomal subunit interfering with bacterial growth [ 85 ]. In the study of Baishya et al, the MIC and minimum biofilm inhibitory concentration was 0.25 and 8 μg/mL, respectively, which shows that concentration superior to 10× MIC was required to eradicate S. aureus biofilms using amikacin as also showed in our study [ 86 ]. Biofilm matrix restricts the of antimicrobials diffusion [ 27 ].…”

Section: Discussionsupporting

confidence: 78%

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

et al. 2022

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This study aimed to compare the biofilm formation ability of Staphylococcus aureus isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 S. aureus strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), spa and agr-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted S. aureus isolates, which may have potential implications for the development of new biofilm-targeted therapeutics.

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Section: Discussionsupporting

confidence: 78%

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

et al. 2022

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“…Following the treatment of sessile cells as control or with monomeric and dimeric Cu II complexes, bacterial growth was determined at 590 nm using a spectrophotometer at varying intervals of time. 66 Determination of EPS Degradation on Being Challenged by Monomeric and Dimeric Cu II Complexes. Biofilms of the working strain were grown on chitin flakes 0.1% (w/v) separately in 100 mL of LB media and centrifuged at 12,000 rpm for 15 min at 4 °C to break the biofilm.…”

Section: ■ Conclusionmentioning

confidence: 99%

In Situ Reactivity of Electrochemically Generated Nitro Radical Anion on Tinidazole and Its Monomeric and Dimeric Cu^II Complexes on Model Biological Targets with Relative Manifestation of Preventing Bacterial Biofilm Formation

et al. 2022

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Formation of nitro radical anion (−NO 2 •− ) and other reduction products of 5-nitroimidazoles, although important for antimicrobial activity, makes the drugs neurotoxic. Hence, an appropriate generation and their role in the free radical pathway needs proper realization. This was attempted by studying the action of tinidazole and its Cu II complexes on model targets (nucleic acid bases and calf thymus DNA). Results obtained were correlated with studies on biological species where prevention of biofilm formation on Staphylococcus aureus and Pseudomonas aeruginosa was followed. Tinidazole and its Cu II complexes subjected to electrochemical reduction in aqueous solution, under de-aerated conditions, interact with model nucleic acid bases and calf thymus DNA. These model targets were followed to realize what happens when such compounds undergo enzymatic reduction within cells of microorganisms that they eventually kill. Studies reveal that Cu II complexes were better in modifying nucleic acid bases and calf thymus DNA than tinidazole; damage caused to nucleic acid bases was correlated with that caused to DNA, indicating that compounds affect DNA rich in thymine and adenine. Minimum bactericidal concentrations on sessile S. aureus and P. aeruginosa for the monomeric Cu II complex were 12.5 and 20.25 μM respectively, while those for the dimeric complex were 40.0 and 45.0 μM, respectively. Biofilm formation by P. aeruginosa and S. aureus and viability count of sessile cells were also determined. Cu II complexes of tinidazole brought about substantial reduction in carbohydrate and protein content in S. aureus and P. aeruginosa. Downregulation of quorum sensing signaling mechanism viz. reduced production of pyocyanin and elastase during biofilm formation was also detected. Cu II complexes showed much higher tendency to prevent biofilm formation than tinidazole, almost comparable to amoxicillin, an established drug in this regard.

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“…The biofilm formation by P. aeruginosa was determined using the 96 well plate incubated at 37°C for a period of 72 h. The wells were initially rinsed with phosphate buffer followed by staining with crystal violet 0Á1% (v/v) for a period of 10 min. This was followed by washing with phosphate buffer saline (PBS) and allowed to dry at 37°C for 1 h. Crystal violet was dissolved in glacial acetic acid 30% (v/v) and spectroscopically measured at 540 nm (Baishya et al 2016).…”

Section: Determination Of Minimum Bactericidal Concentration and Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…The working strain grown on 0Á1% chitin flakes (w/v) for a period of 72 h was washed with 0Á1% (w/v) normal saline to eliminate planktonic groups of cells. After treating the sessile cells with plant extracts and bioactive compounds, the growth of was determined spectrophotometrically at 590 nm at varying intervals of time (Baishya et al 2016).…”

Section: Determination Of the Viability Count Of The Sessile Group Of Cellsmentioning

confidence: 99%

Antibiofilm and anti‐quorum sensing activities of eugenol and linalool from Ocimum tenuiflorum against Pseudomonas aeruginosa biofilm

et al. 2021

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Aims:The aim of this study is to determine the ability of two bioactive compounds, namely, eugenol and linalool, purified from leaves of Ocimum tenuiflorum for eradication of biofilm produced by Pseudomonas aeruginosa. Methods and Results: The phytoextract of O. tenuiflorum (KT), a common ethno-botanical plant of India, was purified through high-performance liquid chromatography and was analysed using ultraviolet (UV) spectroscopy and gas chromatography-mass spectrometry (GC-MS). Eugenol and linalool were found to be the most active amongst all phytocompounds present in phytoextract and showed a significant reduction in the viability of sessile cells of P. aeruginosa and the minimum revival after withdrawal of phyto-challenge. They could bring about notable reduction in the protein and carbohydrate content of exopolysaccharide of biofilm. Eugenol and linalool could affect the synthesis of quorum sensing (QS) proteins like LasA and LasB as well as virulence factors such as pyocyanin, and rhamnolipids, which seriously hamper the formation of biofilm. The biofilm framework was extremely affected by the phytocompounds through the reduction of protein and carbohydrate content of extracellular polymeric substance (EPS). Another interesting found out was that they brought about maximum inhibition to the genomic DNA and RNA content. The studies were supported by in silico interaction between eugenol and linalool with the QS proteins. The antibiofilm efficacies of eugenol, linalool and phytoextract (KT) were further confirmed by microscopic studies with scanning electron microscopy (SEM), atomic force microscopy and fluorescence confocal microscopy microscopic studies. Conclusions: The phytocompounds are proved to be more effective than conventional antibiotics in inhibiting the biofilm forming sessile cells and can be used as a replacement for antibiotic. Significance and Impact of the Study: Pure eugenol extracted from common basil leaves can be used as a safe substitute for common antibiotic for treatment of chronic infections caused by P. aeruginosa. It will be cost effective, devoid of notable side effects and will not generate antibiotic resistance in host body.

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Establishment of a simple reproducible model for antibiotic sensitivity pattern study of biofilm forming staphylococcus aureus

Cited by 13 publications

References 14 publications

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

In Situ Reactivity of Electrochemically Generated Nitro Radical Anion on Tinidazole and Its Monomeric and Dimeric Cu^II Complexes on Model Biological Targets with Relative Manifestation of Preventing Bacterial Biofilm Formation

Antibiofilm and anti‐quorum sensing activities of eugenol and linalool from Ocimum tenuiflorum against Pseudomonas aeruginosa biofilm

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Establishment of a simple reproducible model for antibiotic sensitivity pattern study of biofilm forming staphylococcus aureus

Cited by 13 publications

References 14 publications

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance

In Situ Reactivity of Electrochemically Generated Nitro Radical Anion on Tinidazole and Its Monomeric and Dimeric CuII Complexes on Model Biological Targets with Relative Manifestation of Preventing Bacterial Biofilm Formation

Antibiofilm and anti‐quorum sensing activities of eugenol and linalool from Ocimum tenuiflorum against Pseudomonas aeruginosa biofilm

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In Situ Reactivity of Electrochemically Generated Nitro Radical Anion on Tinidazole and Its Monomeric and Dimeric Cu^II Complexes on Model Biological Targets with Relative Manifestation of Preventing Bacterial Biofilm Formation