2018
DOI: 10.1016/j.anaerobe.2018.06.004
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Establishment of a species-specific primer pair for detecting Veillonella infantium based on the 70 kDa heat shock protein gene dnaK

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Cited by 4 publications
(4 citation statements)
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“…In this study, there were several novelties, including the first time that the RT-qPCR machine was used to analyze oral Veillonella spp. with species-specific primer for Veillonella based on the rpoB and dnaK gene [ 11 , 27 , 28 ] from dental biofilm samples, and for the first time, an analysis of the relationship between the amount of oral Veillonella , stunting status, and oral hygiene index in Indonesia was conducted.…”
Section: Discussionmentioning
confidence: 99%
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“…In this study, there were several novelties, including the first time that the RT-qPCR machine was used to analyze oral Veillonella spp. with species-specific primer for Veillonella based on the rpoB and dnaK gene [ 11 , 27 , 28 ] from dental biofilm samples, and for the first time, an analysis of the relationship between the amount of oral Veillonella , stunting status, and oral hygiene index in Indonesia was conducted.…”
Section: Discussionmentioning
confidence: 99%
“…Specific primer sets of oral Veillonella species were used in this study for the identification of Veillonella species [ 27 , 28 ].…”
Section: Methodsmentioning
confidence: 99%
“…Veillonella is strictly anaerobic and Gram‐negative. Veillonella can be found as a commensal microorganism in the lungs, vagina, mouth and GIT of humans 35 . Veillonella is common and considered mainly harmless 36 .…”
Section: Discussionmentioning
confidence: 99%
“…Previous studies involving the molecular detection of Veillonella were mainly focused at the species level. For example, Rôças and Siqueira Jr (2006) reported the detection of V. parvula by nested PCR, and Mashima and Nakazawa reported the detection of V. tobetsuensis and V. infantium (Mashima and Nakazawa 2013;Mashima et al 2018a) by amplification of the DnaK gene. Although the specificity of species-level detection is high, the detection spectrum is narrow in clinical practice and difficult to apply to the detection of samples containing unknown pathogens.…”
Section: Introductionmentioning
confidence: 99%